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Literature summary for 2.3.2.12 extracted from

  • Cordillot, M.; Dubee, V.; Triboulet, S.; Dubost, L.; Marie, A.; Hugonnet, J.E.; Arthur, M.; Mainardi, J.L.
    In vitro cross-linking of Mycobacterium tuberculosis peptidoglycan by L,D-transpeptidases and inactivation of these enzymes by carbapenems (2013), Antimicrob. Agents Chemother., 57, 5940-5945.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
doripenem
-
 Mycobacterium tuberculosis
ertapenem
-
 Mycobacterium tuberculosis
Imipenem
-
 Mycobacterium tuberculosis
meropenem
-
 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidyl-tRNA1 + aminoacyl-tRNA2 Mycobacterium tuberculosis
-
 tRNA1 + peptidyl(aminoacyl-tRNA2)
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GlcNAc-MurNGlyc-L-Ala1-D-iGln2-meso-DapNH23-D-Ala4 + D-methionine
-
 Mycobacterium tuberculosis GlcNAc-MurNGlyc-L-Ala1-D-iGln2-meso-DapNH23-D-Met4 + D-alanine
-
 ?
additional information no activity with GlcNAc-MurNGlyc-L-Ala1-DiGln2-meso-DapNH23-D-Ala4-D-Ala5 Mycobacterium tuberculosis ?
-
 ?
additional information the five isoforms are active in assays of peptidoglycan cross-linking (Mt5), beta-lactam acylation (Mt3), or both (Mt1, Mt2, and Mt4). Mt3 is the only isoform that is inactive in the crosslinking assay Mycobacterium tuberculosis ?
-
 ?
peptidyl-tRNA1 + aminoacyl-tRNA2
-
 Mycobacterium tuberculosis tRNA1 + peptidyl(aminoacyl-tRNA2)
-
 ?

Synonyms

Synonyms Comment Organism
L,D-transpeptidase
-
 Mycobacterium tuberculosis
LDT
-
 Mycobacterium tuberculosis