Literature summary for 2.3.2.12 extracted from

Michelinaki, M.; Mamos, P.; Coutsogeorgopoulos, C.; Kalpaxis, D.L.
Aminoacyl and peptidyl analogs of chloramphenicol as slow-binding inhibitors of ribosomal peptidyltransferase: a new approach for evaluating their potency (1997), Mol. Pharmacol., 51, 139-146.

Search for more literature for 2.3.2.12

Inhibitors

Inhibitors	Comment	Organism	Structure
chloramphenicol	competitive inhibition with acetylphenylalanyl-tRNA-polyU-ribosome complex, newly formed complex is inactive towards puromycin	Escherichia coli
Gly-chloramphenicol	competitive inhibition with acetylphenylalanyl-tRNA-polyU-ribosome complex, newly formed complex is inactive towards puromycin	Escherichia coli
Gly-Phe-chloramphenicol	competitive inhibition with acetylphenylalanyl-tRNA-polyU-ribosome complex, newly formed complex is inactive towards puromycin	Escherichia coli
L-Phe-chloramphenicol	competitive inhibition with acetylphenylalanyl-tRNA-polyU-ribosome complex, newly formed complex is inactive towards puromycin	Escherichia coli
L-Phe-Gly-chloramphenicol	competitive inhibition with acetylphenylalanyl-tRNA-polyU-ribosome complex, newly formed complex is inactive towards puromycin	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
ribosome	additionally factors washable from ribosomes	Escherichia coli	5840	-

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	B cells, frozen	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
AcPhe-tRNA + puromycin	AcPhe-tRNA-polyU-ribosome complex	Escherichia coli	tRNA + AcPhe-puromycin	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	enzyme assay	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.002	-	L-Phe-Gly-chloramphenicol	-	Escherichia coli
0.0058	-	Gly-chloramphenicol	-	Escherichia coli
0.007	-	chloramphenicol	-	Escherichia coli
0.02	-	L-Phe-chloramphenicol	-	Escherichia coli
0.09	-	Gly-Phe-chloramphenicol	-	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)