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show all sequences of 2.3.1.B6

Structural analysis of WbpE from Pseudomonas aeruginosa PAO1: a nucleotide sugar aminotransferase involved in O-antigen assembly

Larkin, A.; Olivier, N.B.; Imperiali, B.; Biochemistry 49, 7227-7237 (2010)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
the enzymes WbpA, WbpB, WbpE, WbpD and WbpI which act stepwise manner starting from UDP-GlcNAc can be combined in vitro to generate UDP-ManNAc(3NAc)A in a single reaction vessel
Pseudomonas aeruginosa PAO1
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pseudomonas aeruginosa PAO1
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.107
-
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid
30°C, pH 7.0
Pseudomonas aeruginosa PAO1
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa PAO1
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Pseudomonas aeruginosa PAO1
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrate: UDP-GlcNAcA, UDP-GlcNAc, and UDP-2-acetamido-4-amino-2,4,6-trideoxy-D-glucosamine
707534
Pseudomonas aeruginosa PAO1
?
-
-
-
-
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid + acetyl-CoA
-
707534
Pseudomonas aeruginosa PAO1
UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid + CoA
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Pseudomonas aeruginosa PAO1
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4
65
-
Pseudomonas aeruginosa PAO1
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pseudomonas aeruginosa PAO1
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
10
-
Pseudomonas aeruginosa PAO1
Application (protein specific)
Application
Commentary
Organism
synthesis
the enzymes WbpA, WbpB, WbpE, WbpD and WbpI which act stepwise manner starting from UDP-GlcNAc can be combined in vitro to generate UDP-ManNAc(3NAc)A in a single reaction vessel
Pseudomonas aeruginosa PAO1
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudomonas aeruginosa PAO1
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.107
-
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid
30°C, pH 7.0
Pseudomonas aeruginosa PAO1
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Pseudomonas aeruginosa PAO1
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrate: UDP-GlcNAcA, UDP-GlcNAc, and UDP-2-acetamido-4-amino-2,4,6-trideoxy-D-glucosamine
707534
Pseudomonas aeruginosa PAO1
?
-
-
-
-
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid + acetyl-CoA
-
707534
Pseudomonas aeruginosa PAO1
UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid + CoA
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Pseudomonas aeruginosa PAO1
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4
65
-
Pseudomonas aeruginosa PAO1
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pseudomonas aeruginosa PAO1
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
10
-
Pseudomonas aeruginosa PAO1
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
48.3
-
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid
30°C, pH 7.0
Pseudomonas aeruginosa PAO1
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
48.3
-
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid
30°C, pH 7.0
Pseudomonas aeruginosa PAO1
Other publictions for EC 2.3.1.B6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737244
Shoji
Involvement of the Wbp pathway ...
Porphyromonas gingivalis, Porphyromonas gingivalis DSM 20709
Sci. Rep.
4
5056
2014
-
-
-
-
-
-
-
-
-
-
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4
-
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
707534
Larkin
Structural analysis of WbpE fr ...
Pseudomonas aeruginosa PAO1
Biochemistry
49
7227-7237
2010
-
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
1
1
-
-
1
1
-
-
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-
-
1
1
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-
-
-
-
-
1
-
-
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-
-
-
-
1
-
-
-
-
2
-
1
1
-
-
1
1
-
-
-
-
-
-
1
1
709007
Westman
Characterization of WbpB, WbpE ...
Pseudomonas aeruginosa
J. Biol. Chem.
284
11854-11862
2009
-
1
1
-
-
-
-
-
-
-
-
-
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1
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-
-
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2
1
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1
1
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2
1
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-
692874
Westman
Biosynthesis of a rare di-N-ac ...
Pseudomonas aeruginosa
J. Bacteriol.
190
6060-6069
2008
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1
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1
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2
-
1
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1
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-
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2
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-
1
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1
1
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1
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2
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-
1
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2
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