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show all sequences of 2.3.1.B6

Biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharides of both Pseudomonas aeruginosa and Bordetella pertussis occurs via an identical scheme despite different gene clusters

Westman, E.L.; Preston, A.; Field, R.A.; Lam, J.S.; J. Bacteriol. 190, 6060-6069 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene wbpD, expression and subcloning of His6-tagged WbpD in Escherichia coli strain BL21(DE3)
Pseudomonas aeruginosa
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of a gene wbpD knockout strain, functional complementation by expression of WbpB from Bordetella pertussis, overview
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas aeruginosa
the enzyme is involved in the biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharide 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid
?
-
-
-
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid + acetyl-CoA
Pseudomonas aeruginosa
-
UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid + CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
-
gene wbpD
-
Purification (Commentary)
Commentary
Organism
recombinant His6-tagged WbpD from Escherichia coli strain BL21(DE3)
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme is involved in the biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharide 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid
692874
Pseudomonas aeruginosa
?
-
-
-
-
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid + acetyl-CoA
-
692874
Pseudomonas aeruginosa
UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid + CoA
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
acetyl-CoA
-
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
gene wbpD, expression and subcloning of His6-tagged WbpD in Escherichia coli strain BL21(DE3)
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
acetyl-CoA
-
Pseudomonas aeruginosa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of a gene wbpD knockout strain, functional complementation by expression of WbpB from Bordetella pertussis, overview
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas aeruginosa
the enzyme is involved in the biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharide 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid
?
-
-
-
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid + acetyl-CoA
Pseudomonas aeruginosa
-
UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid + CoA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged WbpD from Escherichia coli strain BL21(DE3)
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme is involved in the biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharide 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid
692874
Pseudomonas aeruginosa
?
-
-
-
-
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid + acetyl-CoA
-
692874
Pseudomonas aeruginosa
UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid + CoA
-
-
-
?
Other publictions for EC 2.3.1.B6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737244
Shoji
Involvement of the Wbp pathway ...
Porphyromonas gingivalis, Porphyromonas gingivalis DSM 20709
Sci. Rep.
4
5056
2014
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-
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4
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-
-
-
-
-
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-
-
-
-
-
-
1
1
-
-
-
707534
Larkin
Structural analysis of WbpE fr ...
Pseudomonas aeruginosa PAO1
Biochemistry
49
7227-7237
2010
-
1
1
-
-
-
-
1
-
-
-
-
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1
-
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1
-
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-
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2
-
1
1
-
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1
1
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1
1
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1
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1
-
-
-
-
2
-
1
1
-
-
1
1
-
-
-
-
-
-
1
1
709007
Westman
Characterization of WbpB, WbpE ...
Pseudomonas aeruginosa
J. Biol. Chem.
284
11854-11862
2009
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1
1
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1
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2
1
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1
1
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-
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2
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
692874
Westman
Biosynthesis of a rare di-N-ac ...
Pseudomonas aeruginosa
J. Bacteriol.
190
6060-6069
2008
-
-
1
-
1
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
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-
1
1
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1
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-
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-
-
2
-
-
-
1
-
-
-
-
2
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