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Literature summary for 2.3.1.B41 extracted from

  • Jiang, H.; Khan, S.; Wang, Y.; Charron, G.; He, B.; Sebastian, C.; Du, J.; Kim, R.; Ge, E.; Mostoslavsky, R.; Hang, H.C.; Hao, Q.; Lin, H.
    SIRT6 regulates TNF-alpha secretion through hydrolysis of long-chain fatty acyl lysine (2013), Nature, 496, 110-113.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.B41

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method at 18°C, crystal structure of Sirt6 in complex with a histone H3 K9 myristoyl peptide and ADPribose at 2.2 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
H133Y when human Sirt6 wild-type enzyme is overexpressed in Sirt6 KO MEF cells, tumor necrosis factor alpha has lower fatty acylation level than tumor necrosis factor alpha from cells without overexpression of human Sirt6, while overexpression of human Sirt6 H133Y catalytic mutant does not have much effect on tumor necrosis factor alpha fatty acylation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 [histone H3 peptide]-N6-palmitoyl-L-lysine pH 8.0, 37°C Homo sapiens
0.0024
-
 [synthetic tumor necrosis factor alpha peptide]-N6-myristoyl-L-lysine19 pH 8.0, 37°C Homo sapiens
0.0034
-
 [histone H3 peptide]-N6-myristoyl-L-lysine pH 8.0, 37°C Homo sapiens
0.0045
-
 [synthetic tumor necrosis factor alpha peptide]-N6-myristoyl-L-lysine20 pH 8.0, 37°C Homo sapiens
0.04
-
 [histone H3 peptide]-N6-octanoyl-L-lysine pH 8.0, 37°C Homo sapiens
0.2
-
 [histone H3 peptide]-N6-butyryl-L-lysine pH 8.0, 37°C Homo sapiens
0.81
-
 [histone H3 peptide]-N6-acetyl-L-lysine pH 8.0, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + [tumor necrosis factor alpha]-N6-acyl-L-lysine20 Homo sapiens SIRT6 promotes the secretion of tumor necrosis factor alpha by removing the fatty acyl modification on K19 and K20 of TNFalpha nicotinamide + [tumor necrosis factor alpha]-L-lysine20 + 2'-O-acyl-ADP-ribose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8N6T7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Sirt6 prefers long chain fatty acyl groups. Low deacetylase activity on histone H3 K9 and histone H3 K56. Selectivity for peptide sequence is not high Homo sapiens ?
-
 ?
NAD+ + [histone H3 peptide]-N6-acetyl-L-lysine
-
 Homo sapiens nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
NAD+ + [histone H3 peptide]-N6-butyryl-L-lysine
-
 Homo sapiens nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-butyryl-ADP-ribose
-
 ?
NAD+ + [histone H3 peptide]-N6-myristoyl-L-lysine
-
 Homo sapiens nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?
NAD+ + [histone H3 peptide]-N6-octanoyl-L-lysine
-
 Homo sapiens nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-octanoyl-ADP-ribose
-
 ?
NAD+ + [histone H3 peptide]-N6-palmitoyl-L-lysine
-
 Homo sapiens nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-palmitoyl-ADP-ribose
-
 ?
NAD+ + [synthetic tumor necrosis factor alpha peptide]-N6-myristoyl-L-lysine19
-
 Homo sapiens nicotinamide + [synthetic tumor necrosis factor alpha peptide]-L-lysine19 + 2'-O-myristoyl-ADP-ribose
-
 ?
NAD+ + [synthetic tumor necrosis factor alpha peptide]-N6-myristoyl-L-lysine20
-
 Homo sapiens nicotinamide + [synthetic tumor necrosis factor alpha peptide]-L-lysine20 + 2'-O-myristoyl-ADP-ribose
-
 ?
NAD+ + [tumor necrosis factor alpha]-N6-acyl-L-lysine20 SIRT6 promotes the secretion of tumor necrosis factor alpha by removing the fatty acyl modification on K19 and K20 of TNFalpha Homo sapiens nicotinamide + [tumor necrosis factor alpha]-L-lysine20 + 2'-O-acyl-ADP-ribose
-
 ?
NAD+ + [tumor necrosis factor alpha]-N6-myristoyl-L-lysine
-
 Homo sapiens nicotinamide + [tumor necrosis factor alpha]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?

Synonyms

Synonyms Comment Organism
SIRT6
-
 Homo sapiens
sirtuin 6
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 [synthetic tumor necrosis factor alpha peptide]-N6-myristoyl-L-lysine19 pH 8.0, 37°C Homo sapiens
0.0021
-
 [histone H3 peptide]-N6-butyryl-L-lysine pH 8.0, 37°C Homo sapiens
0.0027
-
 [histone H3 peptide]-N6-palmitoyl-L-lysine pH 8.0, 37°C Homo sapiens
0.0039
-
 [histone H3 peptide]-N6-acetyl-L-lysine pH 8.0, 37°C Homo sapiens
0.0046
-
 [histone H3 peptide]-N6-octanoyl-L-lysine pH 8.0, 37°C Homo sapiens
0.0049
-
 [histone H3 peptide]-N6-myristoyl-L-lysine pH 8.0, 37°C Homo sapiens
0.005
-
 [synthetic tumor necrosis factor alpha peptide]-N6-myristoyl-L-lysine20 pH 8.0, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

General Information

General Information Comment Organism
metabolism SIRT6 promotes the secretion of tumor necrosis factor alpha by removing the fatty acyl modification on K19 and K20 of TNFalpha Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0048
-
 [histone H3 peptide]-N6-acetyl-L-lysine pH 8.0, 37°C Homo sapiens
0.01
-
 [histone H3 peptide]-N6-butyryl-L-lysine pH 8.0, 37°C Homo sapiens
0.12
-
 [histone H3 peptide]-N6-octanoyl-L-lysine pH 8.0, 37°C Homo sapiens
0.14
-
 [histone H3 peptide]-N6-myristoyl-L-lysine pH 8.0, 37°C Homo sapiens
0.83
-
 [synthetic tumor necrosis factor alpha peptide]-N6-myristoyl-L-lysine19 pH 8.0, 37°C Homo sapiens
1.1
-
 [synthetic tumor necrosis factor alpha peptide]-N6-myristoyl-L-lysine20 pH 8.0, 37°C Homo sapiens
3
-
 [histone H3 peptide]-N6-palmitoyl-L-lysine pH 8.0, 37°C Homo sapiens