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Literature summary for 2.3.1.B38 extracted from

  • Drees, S.L.; Li, C.; Prasetya, F.; Saleem, M.; Dreveny, I.; Williams, P.; Hennecke, U.; Emsley, J.; Fetzner, S.
    PqsBC, a condensing enzyme in the biosynthesis of the Pseudomonas aeruginosa quinolone signal: crystal structure, inhibition, and reaction mechanism (2016), J. Biol. Chem., 291, 6610-6624.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant C129A, to 2 A resolution, space group P212121. The heterodimer displays a pseudo 2fold symmetry. Subunit PqsC has an active site comprised of Cys129 and His269 Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
C129A mutation in catalytic dyad, crystallization data Pseudomonas aeruginosa
V299N substitution leads to significant activity toward the deamino substrate analog benzoylacetate, suggesting that the substrate 2-aminobenzoylacetate itself supplies the asparagine-equivalent amino function that assists in catalysis Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
2-aminoacetophenone pathway-inherent competitive inhibitor. EC50 of 46 microM in the in vitro assay Pseudomonas aeruginosa
additional information 2-heptyl-4(1H)-quinolone and 2-heptyl-3-hydroxy-4(1H)-quinolone as well as ,4-dihydroxyquinoline at concentrations up to 50 microM do not affect PqsBC activity Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
octanoyl-CoA pH 8.2, temperature not specified in the publication Pseudomonas aeruginosa
0.105
-
2-aminobenzoylacetate pH 8.2, temperature not specified in the publication Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa the catalytic His269 residue is not involved in acyl-enzyme formation, but contributes to an acylation-dependent increase in affinity for the second substrate 2-aminobenzoylacetate. Octanoylation of Cys129 of PqsC is independent of His269 ?
-
?
octanoyl-CoA + 2-aminobenzoylacetate Pseudomonas aeruginosa
-
CoA + 2-heptylquinolin-4(1H)-one + CO2 + H2O product is a signal molecule in Pseudomonas aeruginosa. The initial product is 1-(2-aminophenyl)decane-1,3-dione which spontaneously rearranges to the product under the elimination of water ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I4X1 subunit PqsC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the catalytic His269 residue is not involved in acyl-enzyme formation, but contributes to an acylation-dependent increase in affinity for the second substrate 2-aminobenzoylacetate. Octanoylation of Cys129 of PqsC is independent of His269 Pseudomonas aeruginosa ?
-
?
octanoyl-CoA + 2-aminobenzoylacetate
-
Pseudomonas aeruginosa CoA + 2-heptylquinolin-4(1H)-one + CO2 + H2O product is a signal molecule in Pseudomonas aeruginosa. The initial product is 1-(2-aminophenyl)decane-1,3-dione which spontaneously rearranges to the product under the elimination of water ?

Synonyms

Synonyms Comment Organism
PqsBC
-
Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.8
-
octanoyl-CoA pH 8.2, temperature not specified in the publication Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.054 0.056 2-aminoacetophenone pH 8.2, temperature not specified in the publication Pseudomonas aeruginosa