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Literature summary for 2.3.1.B33 extracted from

  • Moglia, A.; Lanteri, S.; Comino, C.; Hill, L.; Knevitt, D.; Cagliero, C.; Rubiolo, P.; Bornemann, S.; Martin, C.
    Dual catalytic activity of hydroxycinnamoyl-coenzyme A quinate transferase from tomato allows it to moonlight in the synthesis of both mono- and dicaffeoylquinic acids (2014), Plant Physiol., 166, 1777-1787.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol slight increase in activity Solanum lycopersicum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Solanum lycopersicum

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling based on structure 4KEC of Sorghum bicolor hydroxycinnamoyl-coenzyme A transferase Solanum lycopersicum

Protein Variants

Protein Variants Comment Organism
H276Y 58% of wild-type activity for production of dicaffeoylquinic acids, 92% of wild-type hydroxycinnamoyltranferase activity Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
19
-
chlorogenic acid pH 4.0, 30°C Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm enzyme catalyzes different reactions in different compartments Solanum lycopersicum 5737
-
vacuole enzyme catalyzes different reactions in different compartments Solanum lycopersicum 5773
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ slight increase in activity Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 chlorogenic acid Solanum lycopersicum reaction is irreversible 1,5-dicaffeoylquinic acid + quinate products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid ir
2 chlorogenic acid Solanum lycopersicum reaction is irreversible 3,5-dicaffeoylquinic acid + quinate products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid ir
2 chlorogenic acid Solanum lycopersicum reaction is irreversible 4,5-dicaffeoylquinic acid + quinate products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid ir

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum Q70G32 bifunctional enzyme, displays hydroxycinnamoyl-Coenzyme A:quinate hydroxycinnamoyl transferase activity and chlorogenate:chlorogenate transferase activity
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit increase in CCT activity is observed during ripening, reaching a maximum in the peel of red fruit Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22.3
-
pH 4.0, 30°C Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 chlorogenic acid reaction is irreversible Solanum lycopersicum 1,5-dicaffeoylquinic acid + quinate products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid ir
2 chlorogenic acid reaction is irreversible Solanum lycopersicum 3,5-dicaffeoylquinic acid + quinate products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid ir
2 chlorogenic acid reaction is irreversible Solanum lycopersicum 4,5-dicaffeoylquinic acid + quinate products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid ir

Synonyms

Synonyms Comment Organism
CCT
-
Solanum lycopersicum
chlorogenate:chlorogenate transferase
-
Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Solanum lycopersicum

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor required Solanum lycopersicum