Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.B32 extracted from

  • Dunn, B.J.; Watts, K.R.; Robbins, T.; Cane, D.E.; Khosla, C.
    Comparative analysis of the substrate specificity of trans- versus cis-acyltransferases of assembly line polyketide synthases (2014), Biochemistry, 53, 3796-3806.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.B32

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 methylmalonyl-CoA pH 7.6, temperature not specified in the publication Saccharopolyspora erythraea

Organism

Organism UniProt Comment Textmining
Saccharopolyspora erythraea Q03132 Q03132, i.e. modules 3 and 4, of 6-deoxyerythronolide B synthase, EC 2.3.1.94
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethylmalonyl-CoA + an [acyl-carrier protein]
-
 Saccharopolyspora erythraea CoA + a methylmalonyl-[acyl-carrier protein]
-
 ?
malonyl-CoA + an [acyl-carrier protein]
-
 Saccharopolyspora erythraea CoA + a methylmalonyl-[acyl-carrier protein]
-
 ?
methylmalonyl-CoA + an [acyl-carrier protein]
-
 Saccharopolyspora erythraea CoA + a methylmalonyl-[acyl-carrier protein]
-
 ?
additional information the cis-acyltransferase domain from DEBS module 3 transfers a methylmalonyl extender unit from the corresponding carboxyacyl-CoA precursor onto the phosphopantetheine arm of the acyl carrier protein within the same module. The presence of the acyl carrier protein has little effect on the specificity of the cis-acyltransferase domain of 6-deoxyerythronolide B synthase for carboxyacyl-CoA substrates, but has a marked influence on the corresponding specificity parameters for the trans-acyltransferases from the disorazole and kirromycin synthases. Comparative analysis of the substrate specificity Saccharopolyspora erythraea ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.033
-
 methylmalonyl-CoA pH 7.6, temperature not specified in the publication Saccharopolyspora erythraea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.8
-
 ethylmalonyl-CoA below 0.8 per sec and mM, pH 7.6, temperature not specified in the publication Saccharopolyspora erythraea
0.8
-
 malonyl-CoA pH 7.6, temperature not specified in the publication Saccharopolyspora erythraea
16.7
-
 methylmalonyl-CoA pH 7.6, temperature not specified in the publication Saccharopolyspora erythraea