BRENDA - Reference to 2.3.1.B2; Id = 718922

construction of an N-terminally Strep2-tagged PhaC, Strep2-PhaCRe, and integration into the Ralstonia eutropha genome in place of wild-type phaC and functional expression without a lag phase of CoA release in the enzyme reaction, functional expression of Strep2-PhaCRe in Escherichia coli strain BL21(DE3) showing a lag phase in CoA release

Cupriavidus necator

C319A

site-directed mutagenesis

Cupriavidus necator

64000

-

1 * 64000, PhaC, SDS-PAGE; 2 * 64000, PhaC, SDS-PAGE

Cupriavidus necator

3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n

Cupriavidus necator

-

[(R)-3-hydroxybutanoate]n+1 + CoA

-

?

3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n

Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1

-

[(R)-3-hydroxybutanoate]n+1 + CoA

-

?

Cupriavidus necator

-

Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1

-

recombinant N-terminally Strep2-tagged PhaC from Ralstonia eutropha by affinity chromatography. Strep2-PhaCRe co-purifies with the phasin protein, PhaP1, and with soluble polyhydroxybutyrate of 350 kDa MW in a high-molecular weight complex and in monomeric/dimeric forms with no associated PhaP1 or polyhydroxybutyrate

Cupriavidus necator

3

8

pooled monomeric and dimeric fractions of Strep2-PhaCRe, 30°C, pH not specified in the publication

Cupriavidus necator

36

-

purified recombinant Strep2-PhaCRe, 30°C, pH not specified in the publication

Cupriavidus necator

3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n

-

718922

Cupriavidus necator

[(R)-3-hydroxybutanoate]n+1 + CoA

-

?

3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n

-

718922

Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1

[(R)-3-hydroxybutanoate]n+1 + CoA

-

?

dimer

2 * 64000, PhaC, SDS-PAGE

Cupriavidus necator

monomer

1 * 64000, PhaC, SDS-PAGE

Cupriavidus necator

30

-

assay at

Cupriavidus necator

construction of an N-terminally Strep2-tagged PhaC, Strep2-PhaCRe, and integration into the Ralstonia eutropha genome in place of wild-type phaC and functional expression without a lag phase of CoA release in the enzyme reaction, functional expression of Strep2-PhaCRe in Escherichia coli strain BL21(DE3) showing a lag phase in CoA release

Cupriavidus necator

C319A

site-directed mutagenesis

Cupriavidus necator

64000

-

1 * 64000, PhaC, SDS-PAGE; 2 * 64000, PhaC, SDS-PAGE

Cupriavidus necator

3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n

Cupriavidus necator

-

[(R)-3-hydroxybutanoate]n+1 + CoA

-

?

3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n

Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1

-

[(R)-3-hydroxybutanoate]n+1 + CoA

-

?

recombinant N-terminally Strep2-tagged PhaC from Ralstonia eutropha by affinity chromatography. Strep2-PhaCRe co-purifies with the phasin protein, PhaP1, and with soluble polyhydroxybutyrate of 350 kDa MW in a high-molecular weight complex and in monomeric/dimeric forms with no associated PhaP1 or polyhydroxybutyrate

Cupriavidus necator

3

8

pooled monomeric and dimeric fractions of Strep2-PhaCRe, 30°C, pH not specified in the publication

Cupriavidus necator

36

-

purified recombinant Strep2-PhaCRe, 30°C, pH not specified in the publication

Cupriavidus necator

3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n

-

718922

Cupriavidus necator

[(R)-3-hydroxybutanoate]n+1 + CoA

-

?

3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n

-

718922

Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1

[(R)-3-hydroxybutanoate]n+1 + CoA

-

?

dimer

2 * 64000, PhaC, SDS-PAGE

Cupriavidus necator

monomer

1 * 64000, PhaC, SDS-PAGE

Cupriavidus necator

30

-

assay at

Cupriavidus necator

physiological function

class I PHB synthase, PhaC, from Ralstonia eutropha catalyzes the formation of PHB from (R)-3-hydroxybutyryl-CoA, ultimately resulting in the formation of insoluble granules, the polymer elongation rate is much faster than the initiation rate

Cupriavidus necator

physiological function

class I PHB synthase, PhaC, from Ralstonia eutropha catalyzes the formation of PHB from (R)-3-hydroxybutyryl-CoA, ultimately resulting in the formation of insoluble granules, the polymer elongation rate is much faster than the initiation rate

Cupriavidus necator

735683

Buckley

Chemistry with an artificial p ...

Caulobacter vibrioides

Biochemistry

54

2117-2125

2015

-

2

-

1

-

1

-

736527

Tai

Discovery of a new polyhydroxy ...

uncultured bacterium, uncultured bacterium SC8

J. Biosci. Bioeng.

121

355-364

2015

-

1

-

6

-

1

-

1

-

735350

Zhang

Mechanistic insight with HBCH2 ...

Cupriavidus necator

ACS Chem. Biol.

9

1773-1779

2014

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

735855

Foong

Whole genome amplification app ...

Marinobacter sp., Marinobacter sp. C1S70

BMC Microbiol.

14

318

2014

-

2

-

1

-

735485

Thomson

Efficient production of active ...

Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1

Appl. Environ. Microbiol.

79

1948-1955

2013

-

1

-

5

-

1

-

1

-

1

-

735535

Ochi

Engineering of class i lactate ...

Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1

Appl. Microbiol. Biotechnol.

97

3441-3447

2013

-

1

-

2

-

5

-

1

-

2

-

736535

Park

Propionyl-CoA dependent biosyn ...

Pseudomonas sp., Pseudomonas sp. MBEL 6–19

J. Biotechnol.

165

93-98

2013

-

1

-

1

-

7

-

1

-

1

-

718922

Cho

Purification of polyhydroxybut ...

Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1

Biochemistry

51

2276-2288

2012

-

1

-

1

-

1

2

-

42

-

1

-

2

-

2

1

-

1

-

1

-

1

2

-

1

-

2

-

2

1

-

1

-

714539

Matsumoto

A new pathway for poly(3-hydro ...

Cupriavidus necator

Biosci. Biotechnol. Biochem.

75

364-366

2011

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

719116

Shozui

A new beneficial mutation in P ...

Pseudomonas sp., Pseudomonas sp. 61-3

Biosci. Biotechnol. Biochem.

74

1710-1712

2010

-

1

-

11

-

4

-

6

-

8

-

1

-

11

-

4

-

8

-

684590

Zheng

Mutation on N-terminus of poly ...

Cupriavidus necator

Appl. Microbiol. Biotechnol.

72

896-905

2006

-

13

-

2

-

1

-

13

-

1

-

685489

Matsumoto

Enhancement of poly(3-hydroxyb ...

Aeromonas caviae

Biomacromolecules

6

2126-2130

2005

-

1

-

6

-

1

-

685729

Normi

Site-directed saturation mutag ...

Cupriavidus necator

Biotechnol. Lett.

27

705-712

2005

-

19

-

2

-

1

-

19

-

1

-

688783

Normi

Characterization and propertie ...

Cupriavidus necator

Macromol. Biosci.

5

197-206

2005

-

1

-

21

-

2

-

1

-

3

-

1

-

1

-

2

-

1

-

21

-

2

-

1

-

1

-

1

-

2

-

685487

Zhang

Mechanism of the polymerizatio ...

Cupriavidus necator

Biomacromolecules

4

504-509

2003

-

1

-

3

-

1

-

1

-

1

-

685312

Rehm

Molecular characterization of ...

Cupriavidus necator

Biochim. Biophys. Acta

1594

178-190

2002

-

1

-

11

-

4

-

1

-

1

-

11

-

1

-

684662

Yuan

Class I and III polyhydroxyalk ...

Cupriavidus necator

Arch. Biochem. Biophys.

394

87-98

2001

-

1

-

3

-

1

-

3

-

1

-

1

-

3

-

685059

Jia

Mechanistic studies on class I ...

Cupriavidus necator

Biochemistry

40

1011-1019

2001

-

4

-

1

-

1

-

4

-

1

-

685486

Song

In vitro polymerization and co ...

Cupriavidus necator

Biomacromolecules

1

433-439

2000

-

1

-

1

-

3

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

687130

Kichise

Biosynthesis of polyhydroxyalk ...

Aeromonas caviae

Int. J. Biol. Macromol.

25

69-77

1999

-

1

-

3

-

1

-

Purification of polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications for the initiation and elongation of polymer formation in vivo

Data extracted from this reference: