BRENDA - Enzyme Database show
show all sequences of 2.3.1.B2

Biosynthesis of polyhydroxyalkanoates (PHA) by recombinant Ralstonia eutropha and effects of PHA synthase activity on in vivo PHA biosynthesis

Kichise, T.; Fukui, T.; Yoshida, Y.; Doi, Y.; Int. J. Biol. Macromol. 25, 69-77 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
construction of a recombinant Ralstonia eutropha PHB-4 harboring Aeromonas caviae biosynthesis genes under the control of a promoter for Ralstonia eutropha phb operon (phbRe promoter), and examination of the polyhydroxyalkanoate producing ability of the recombinants from various alkanoic acids as carbon sources. The polymerization step is not the rate-determining one in PHA biosynthesis by Ralstonia eutropha. The molecular weights of poly(3-hydroxybutyrate) produced by the recombinant strains are also independent of the levels of PHA synthase activity
Aeromonas caviae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aeromonas caviae
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
construction of a recombinant Ralstonia eutropha PHB-4 harboring Aeromonas caviae biosynthesis genes under the control of a promoter for Ralstonia eutropha phb operon (phbRe promoter), and examination of the polyhydroxyalkanoate producing ability of the recombinants from various alkanoic acids as carbon sources. The polymerization step is not the rate-determining one in PHA biosynthesis by Ralstonia eutropha. The molecular weights of poly(3-hydroxybutyrate) produced by the recombinant strains are also independent of the levels of PHA synthase activity
Aeromonas caviae
Other publictions for EC 2.3.1.B2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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718922
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2012
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1
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684590
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2006
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685489
Matsumoto
Enhancement of poly(3-hydroxyb ...
Aeromonas caviae
Biomacromolecules
6
2126-2130
2005
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1
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6
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685729
Normi
Site-directed saturation mutag ...
Cupriavidus necator
Biotechnol. Lett.
27
705-712
2005
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19
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688783
Normi
Characterization and propertie ...
Cupriavidus necator
Macromol. Biosci.
5
197-206
2005
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1
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21
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685487
Zhang
Mechanism of the polymerizatio ...
Cupriavidus necator
Biomacromolecules
4
504-509
2003
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1
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3
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685312
Rehm
Molecular characterization of ...
Cupriavidus necator
Biochim. Biophys. Acta
1594
178-190
2002
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1
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11
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11
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684662
Yuan
Class I and III polyhydroxyalk ...
Cupriavidus necator
Arch. Biochem. Biophys.
394
87-98
2001
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1
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685059
Jia
Mechanistic studies on class I ...
Cupriavidus necator
Biochemistry
40
1011-1019
2001
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4
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685486
Song
In vitro polymerization and co ...
Cupriavidus necator
Biomacromolecules
1
433-439
2000
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1
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1
1
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687130
Kichise
Biosynthesis of polyhydroxyalk ...
Aeromonas caviae
Int. J. Biol. Macromol.
25
69-77
1999
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