Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.B16 extracted from

  • Walker, K.; Fujisaki, S.; Long, R.; Croteau, R.
    Molecular cloning and heterologous expression of the C-13 phenylpropanoid side chain-CoA acyltransferase that functions in Taxol biosynthesis (2002), Proc. Natl. Acad. Sci. USA, 99, 12715-12720.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.B16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Taxus cuspidata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
 baccatin III pH and temperature not specified in the publication Taxus cuspidata
0.0049
-
 3-amino-3-phenylpropanoyl-CoA pH and temperature not specified in the publication Taxus cuspidata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Taxus cuspidata
50546
-
 x * 50546, calculated from sequence Taxus cuspidata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
baccatin III + 3-amino-3-phenylpropanoyl-CoA Taxus cuspidata the enzyme is involved in taxol biosynthesis 3'-N-debenzoyl-2'-deoxytaxol + coenzyme A
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus cuspidata Q8H2B5
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Taxus cuspidata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
baccatin III + 3-amino-3-phenylpropanoyl-CoA the enzyme is involved in taxol biosynthesis Taxus cuspidata 3'-N-debenzoyl-2'-deoxytaxol + coenzyme A
-
 ?
baccatin III + 3-amino-3-phenylpropanoyl-CoA selective for 3-amino-3-phenylpropanoyl-CoA esters with a preference for beta-phenylalanoyl-CoA over 3-phenylisoserinoyl-CoA. alpha-Phenylalanoyl-CoA and N-benzoyl phenylisoserinoyl-CoA are not productive acyl donors Taxus cuspidata 3'-N-debenzoyl-2'-deoxytaxol + coenzyme A this enzymatic product is converted to 2'-deoxytaxol by chemical N-benzoylation, and the identity of this derivative is confirmed by spectrometric analyses ?
baccatin III + 3-phenylisoserinoyl-CoA selective for 3-amino-3-phenylpropanoyl-CoA esters with a preference for beta-phenylalanoyl-CoA over 3-phenylisoserinoyl-CoA. Activity with 3-phenylisoserinoyl-CoA is 40% of the activity with beta-phenylalanoyl-CoA. alpha-Phenylalanoyl-CoA and N-benzoyl phenylisoserinoyl-CoA are not productive acyl donors Taxus cuspidata ? + coenzyme A
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Taxus cuspidata
? x * 50546, calculated from sequence Taxus cuspidata

Synonyms

Synonyms Comment Organism
baccatin III 13-O-(3-amino-3-phenylpropanoyl)transferase
-
 Taxus cuspidata
TAX7
-
 Taxus cuspidata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
31
-
 assay at Taxus cuspidata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 recombinant enzyme Taxus cuspidata