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Literature summary for 2.3.1.98 extracted from
- Properties and activity changes of chlorogenic acid:glucaric acid caffeoyltransferase from tomato (Lycopersicon esculentum) (1990), Plant Physiol., 92, 41-47.
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing causes 20-30% loss of activity | Solanum lycopersicum |
| purification has to be carried out at 4°C | Solanum lycopersicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | p-chloromercuribenzoate is no inhibitor | Solanum lycopersicum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
glucaric acid | - |
Solanum lycopersicum |  |
| 1.7 | - |
galactaric acid | - |
Solanum lycopersicum | |
| 20 | - |
chlorogenic acid | - |
Solanum lycopersicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 150% of activity compared to the activity without addition of divalent ions | Solanum lycopersicum | |
| Mg2+ | 125% of activity compared to the activity without addition of divalent ions | Solanum lycopersicum | |
| additional information | EDTA has no effect | Solanum lycopersicum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
SDS-PAGE, gel filtration | Solanum lycopersicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-O-caffeoylquinic acid + galactaric acid | Solanum lycopersicum | - |
quinate + caffeoylgalactaric acid | - |
? | |
| 5-O-caffeoylquinic acid + glucaric acid | Solanum lycopersicum | - |
quinate + caffeoylglucaric acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | - |
tomato | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2400fold | Solanum lycopersicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Solanum lycopersicum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Solanum lycopersicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several months, no apparent loss of activity with crude extract or purified enzyme | Solanum lycopersicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-O-caffeoylquinic acid + galactaric acid | - |
Solanum lycopersicum | quinate + caffeoylgalactaric acid | - |
? | |
| 5-O-caffeoylquinic acid + glucaric acid | - |
Solanum lycopersicum | quinate + caffeoylglucaric acid | - |
? | |
| 5-O-caffeoylquinic acid + glucaric acid | i.e. chlorogenic acid | Solanum lycopersicum | quinate + caffeoylglucaric acid | - |
? | |
| chlorogenic acid + glucarate | - |
Solanum lycopersicum | quinate + 2-O-caffeoylglucarate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
- |
Solanum lycopersicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | - |
- |
Solanum lycopersicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | 6.2 | 50% of maximal activity at pH 4.2 and pH 6.2 | Solanum lycopersicum |
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