Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide | inhibits growth of Trypanosoma cruzi epimastigotes with EC50 of 6.9 microM, but is about 1000fold less potent than in Trypanosma brucei | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0062 | - |
myristoyl-CoA | pH 7.4, 30°C | Trypanosoma cruzi | |
0.012 | - |
CAP5.5 peptide | source Trypanosoma cruzi, pH 7.4, 30°C | Trypanosoma cruzi | |
0.25 | - |
CAP5.5 peptide | source Trypanosoma brucei, pH 7.4, 30°C | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47400 | - |
gel filtration | Trypanosoma cruzi |
53700 | - |
1 * 53700, calculated, 1 * 53700, mass spectrometry | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myristoyl-CoA + CAP5.5 peptide | - |
Trypanosoma cruzi | N-myristoylglycyl-CAP5.5 peptide + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 53700, calculated, 1 * 53700, mass spectrometry | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
CAP5.5 peptide | source Trypanosoma cruzi, pH 7.4, 30°C | Trypanosoma cruzi | |
0.34 | - |
myristoyl-CoA | pH 7.4, 30°C | Trypanosoma cruzi | |
2.11 | - |
CAP5.5 peptide | source Trypanosoma brucei, pH 7.4, 30°C | Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000228 | - |
2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide | pH 7.4, 30°C | Trypanosoma cruzi |
General Information | Comment | Organism |
---|---|---|
physiological function | expression of NMT gene is essential for survival of Trypanosoma cruzi epimastigotes | Trypanosoma cruzi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.44 | - |
CAP5.5 peptide | source Trypanosoma brucei, pH 7.4, 30°C | Trypanosoma cruzi | |
12.4 | - |
CAP5.5 peptide | source Trypanosoma cruzi, pH 7.4, 30°C | Trypanosoma cruzi | |
54.8 | - |
myristoyl-CoA | pH 7.4, 30°C | Trypanosoma cruzi |