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Literature summary for 2.3.1.97 extracted from
- Effects of HIV-1 Nef on human N-myristoyltransferase 1 (2011), Biochemistry, 50, 3394-3403.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His6-tagged Nef:NMT complex in Escherichia coli Codon Plus (DE-)-RIL cells | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | associated at the cytoplasmic side of the membrane | Homo sapiens | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isozyme NMT-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged Nef:NMT complex by nickel affinity chromatography, two proteins are able to remain associated through immunoprecipitation and | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myristoyl-CoA + Nef protein | HIV-1 accessory protein Nef, usage of C-terminally 6xHis-tagged Nef SF2 | Homo sapiens | CoA + N-myristoyl-Nef protein | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| N-myristoyltransferase 1 | - |
Homo sapiens |
| NMT-1 | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | human NMT-1 specifically myristoylates Nef and the two proteins are able to remain associated through immunoprecipitation and purification steps, Nef:NMT complex structure analysis, overview | Homo sapiens |
| physiological function | the HIV-1 accessory protein Nef is N-terminally myristoylated and this posttranslational modification is essential for Nef function in AIDS progression, Nef has no known catalytic activity but rather binds to a number of host-cell proteins. Transfer of a myristate group from myristoyl coenzyme A to Nef occurs cotranslationally and is catalyzed by human N-myristoyl transferase-1. Myristoylated Nef adopts a different tertiary and quaternary structure than non-myristoylated Nef, but conformational changes in Nef are no result of myristoylation, overview. NMT might chaperone Nef to the membrane and thereby protect the myristic acid group from the cytosol rather than Nef operating through a myristic acid switch mechanism | Homo sapiens |
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