Literature summary for 2.3.1.97 extracted from

Panethymitaki, C.; Bowyer, P.W.; Price, H.P.; Leatherbarrow, R.J.; Brown, K.A.; Smith, D.F.
Characterization and selective inhibition of myristoyl-CoA:protein N-myristoyltransferase from Trypanosoma brucei and Leishmania major (2006), Biochem. J., 396, 277-285.

Search for more literature for 2.3.1.97

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Trypanosoma brucei

Inhibitors

Inhibitors	Comment	Organism	Structure
CP-014553	-	Leishmania major
CP-014553	IC50: 460 nM	Trypanosoma brucei
CP-030890-27	-	Leishmania major
CP-030890-27	IC50: 250 nM	Trypanosoma brucei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00137	-	myristoyl-CoA	-	Leishmania major
0.00178	-	myristoyl-CoA	-	Trypanosoma brucei
0.0113	-	GCGGSKVK	-	Trypanosoma brucei
0.11	-	GQLFTSLN	-	Leishmania major

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50500	-	x * 50500, SDS-PAGE	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Leishmania major	Q9GPZ4	-	-
Trypanosoma brucei	Q388H8	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Trypanosoma brucei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myristoyl-CoA + GCGGSKVK	-	Trypanosoma brucei	N-myristoylglycyl-CGGSKVK + CoA	-	?
myristoyl-CoA + GQLFTSLN	-	Leishmania major	N-myristoylglycyl-GQLFTSLN + CoA	-	?

Subunits

Subunits	Comment	Organism
?	x * 50500, SDS-PAGE	Trypanosoma brucei

Synonyms

Synonyms	Comment	Organism
LmNMT	-	Leishmania major
myristoyl-CoA:protein N-myristoyltransferase	-	Leishmania major
myristoyl-CoA:protein N-myristoyltransferase	-	Trypanosoma brucei
TbNMT	-	Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
18.2	-	GCGGSKVK	-	Trypanosoma brucei
23.6	-	myristoyl-CoA	-	Leishmania major
24	-	GQLFTSLN	-	Leishmania major
76.8	-	myristoyl-CoA	-	Trypanosoma brucei

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00025	-	IC50: 250 nM	Trypanosoma brucei	CP-030890-27
0.00046	-	IC50: 460 nM	Trypanosoma brucei	CP-014553

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Release 2023.1 (January 2023)