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Literature summary for 2.3.1.97 extracted from
- Molecular cloning, genomic organization, and biochemical characterization of myristoyl-CoA:protein N-myristoyltransferase from Arabidopsis thaliana (2000), J. Biol. Chem., 275, 9673-9683.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 18% activation at 1 mM | Arabidopsis thaliana |  |
| Triton X-100 | 25% activation at 0.1% | Arabidopsis thaliana | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression in Escherichia coli as His-tagged protein, DNA sequence analysis | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | transgenic plants expressing the enzyme under control of the cauliflower mosaic virus 35S promotor, down-regulation by anti-sense expression leads to a growth reduced or lethal phenotype | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| factor NIP71 | bovine brain inhibitor protein | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km for several protein-derived peptides | Arabidopsis thaliana | |
| 0.0205 | - |
Arabidopsis thaliana protein CDPK6-derived peptide | recombinant enzyme | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Arabidopsis thaliana | 5829 | - |
| mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
| ribosome | major part | Arabidopsis thaliana | 5840 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid sequence, alignment | Arabidopsis thaliana |
| 50000 | - |
1 * 50000, recombinant enzyme | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myristoyl-CoA + glycylpeptide | Arabidopsis thaliana | essential for growth | N-myristoylglycylpeptide + CoA | - |
? | |
| myristoyl-CoA + protein | Arabidopsis thaliana | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9LTR9 | gene nmt1, 2 gene copies on different chromosomes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
| silique | - |
Arabidopsis thaliana | - |
| stem | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00115 | - |
purified recombinant enzyme | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lauroyl-CoA + glycylpeptide | low activity | Arabidopsis thaliana | N-lauroylglycylpeptide + CoA | - |
? | |
| myristoyl-CoA + Arabidopsis thaliana protein CDPK6-derived peptide | - |
Arabidopsis thaliana | N-myristoylated Arabidopsis thaliana protein CDPK6-derived peptide + CoA | - |
? | |
| myristoyl-CoA + Fen kinase-derived peptide | plant protein peptide | Arabidopsis thaliana | N-myristoylated Fen kinase-derived peptide + CoA | - |
? | |
| myristoyl-CoA + glycylpeptide | substrate specificity | Arabidopsis thaliana | N-myristoylglycylpeptide + CoA | - |
? | |
| myristoyl-CoA + glycylpeptide | altered fatty acid chain length | Arabidopsis thaliana | N-myristoylglycylpeptide + CoA | - |
? | |
| myristoyl-CoA + glycylpeptide | specific for myristoyl-CoA | Arabidopsis thaliana | N-myristoylglycylpeptide + CoA | - |
? | |
| myristoyl-CoA + glycylpeptide | essential for growth | Arabidopsis thaliana | N-myristoylglycylpeptide + CoA | - |
? | |
| myristoyl-CoA + p60src-derived peptide | - |
Arabidopsis thaliana | N-myristoylated p60src-derived peptide + CoA | - |
? | |
| myristoyl-CoA + protein | - |
Arabidopsis thaliana | ? | - |
? | |
| n-decanoyl-CoA + glycylpeptide | low activity | Arabidopsis thaliana | N-decanoylglycylpeptide + CoA | - |
? | |
| palmitoyl-CoA + glycylpeptide | poor substrate | Arabidopsis thaliana | N-palmitoylglycylpeptide + CoA | - |
? | |
| stearoyl-CoA + glycylpeptide | poor substrate | Arabidopsis thaliana | N-stearoylglycylpeptide + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, recombinant enzyme | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NMT | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | - |
Arabidopsis thaliana |
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