Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | 2.5fold activation at 5% v/v | Homo sapiens | |
ethanol | 5fold activation at 10% v/v | Homo sapiens | |
SDS | 3.2fold activation at 1.73 mM | Homo sapiens | |
Tris | activates about 2.5fold at 225 mM compared to 40 mM | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
construction of expression plasmid encoding the enzyme, lacking the first 9 amino acid residues, fused to a enterokinase cleavage site and a polyhistidine tag, overexpression in Escherichia coli DH5alpha | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
factor NIP71 | bovine brain inhibitor protein; strong inhibition in a concentration dependent manner, noncompetitive | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
1 * 49000, tag cleaved off by enterokinase, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Oxidation Stability | Organism |
---|---|
acetonitrile, stable in 5% v/v | Homo sapiens |
ethanol, stable in 10% v/v | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.248 | - |
purified wild-type enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myristoyl-CoA + cAMP-dependent protein kinase-derived peptide | - |
Homo sapiens | N-myristoylated cAMP-dependent protein kinase-derived peptide + CoA | - |
? | |
myristoyl-CoA + Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg | pp60src-derived peptide | Homo sapiens | N-myristoyl-Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg + CoA | - |
? | |
myristoyl-CoA + glycylpeptide | specific for Gly at N-terminus | Homo sapiens | N-myristoylglycylpeptide + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 49000, tag cleaved off by enterokinase, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
NMT | - |
Homo sapiens |