Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
expression of mutant nmt1-181 in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D451K | site-directed mutagenesis, no functional complementation of enzyme deficient nmt1-181 mutant at 36°C | Saccharomyces cerevisiae |
D451N | site-directed mutagenesis, no functional complementation of enzyme deficient nmt1-181 mutant at 36°C | Saccharomyces cerevisiae |
E167K | site-directed mutagenesis, coexpression of wild-type with Asp451, functional complementation of enzyme deficient nmt1-181 mutant at 36°C | Saccharomyces cerevisiae |
E293K | site-directed mutagenesis, coexpression of wild-type with Asp451, functional complementation of enzyme deficient nmt1-181 mutant at 36°C | Saccharomyces cerevisiae |
additional information | temperature sensitive mutant strain nmt1-181, exchange of Gly to Asp, 10fold increased Km for myristoyl-CoA at 36°C | Saccharomyces cerevisiae |
additional information | disruption or deletion of nmt1-gene causes recessive lethality | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myristoyl-CoA + glycylpeptide | Saccharomyces cerevisiae | - |
N-myristoylglycylpeptide + CoA | - |
ir | |
myristoyl-CoA + protein | Saccharomyces cerevisiae | natural protein substrates are: catalytic subunit of cAMP-dependent protein kinase, p60src, phosphatases, e.g. calcineurin B, transmembrane signalling proteins, e.g. alpha-subunits of heterotrimeric G proteins, gag polyprotein precursors of several retroviruses, capsid proteins of some parvoviruses and picornaviruses | ? | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Escherichia coli | - |
- |
- |
Saccharomyces cerevisiae | - |
gene nmt1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myristoyl-CoA + glycylpeptide | - |
Saccharomyces cerevisiae | N-myristoylglycylpeptide + CoA | - |
ir | |
myristoyl-CoA + glycylpeptide | stereochemical requirements | Saccharomyces cerevisiae | N-myristoylglycylpeptide + CoA | - |
ir | |
myristoyl-CoA + glycylpeptide | specific for Gly at N-terminus | Saccharomyces cerevisiae | N-myristoylglycylpeptide + CoA | - |
ir | |
myristoyl-CoA + glycylpeptide | specific for myristoyl-CoA | Saccharomyces cerevisiae | N-myristoylglycylpeptide + CoA | - |
ir | |
myristoyl-CoA + glycylpeptide | p60src-derived peptides | Saccharomyces cerevisiae | N-myristoylglycylpeptide + CoA | - |
ir | |
myristoyl-CoA + protein | natural protein substrates are: catalytic subunit of cAMP-dependent protein kinase, p60src, phosphatases, e.g. calcineurin B, transmembrane signalling proteins, e.g. alpha-subunits of heterotrimeric G proteins, gag polyprotein precursors of several retroviruses, capsid proteins of some parvoviruses and picornaviruses | Saccharomyces cerevisiae | ? | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
NMT | - |
Saccharomyces cerevisiae |