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Literature summary for 2.3.1.91 extracted from

  • Shirley, A.M.; Chapple, C.
    Biochemical characterization of sinapoylglucose:choline sinapoyltransferase, a serine carboxypeptidase-like protein that functions as an acyltransferase in plant secondary metabolism (2003), J. Biol. Chem., 278, 19870-19877.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in a Saccharomyces cerevisiae expression system that lacks the carboxypeptodase Y gene and is defective in vacuolar targeting, resulting in secretion of heterologously expressed SCT Arabidopsis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
1-O-caffeoyl-beta-D-glucose pH 7.0, 30°C Arabidopsis sp.
0.16
-
1-O-sinapoyl-beta-D-glucose pH 7.0, 30°C Arabidopsis sp.
0.41
-
1-O-feruloyl-beta-D-glucose pH 7.0, 30°C Arabidopsis sp.
1.9
-
p-coumaroyl-beta-D-glucose pH 7.0, 30°C Arabidopsis sp.
3.2
-
choline pH 7.0, 30°C Arabidopsis sp.
19
-
N,N-dimethylethanolamine pH 7.0, 30°C Arabidopsis sp.
25
-
neopentyl alcohol pH 7.0, 30°C Arabidopsis sp.
105
-
Tris pH 7.0, 30°C Arabidopsis sp.
245
-
2-Methylaminoethanol pH 7.0, 30°C Arabidopsis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-O-sinapoyl-beta-D-glucose + choline Arabidopsis sp. enzyme functions in sinapate ester synthesis D-glucose + sinapoylcholine
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the 50000 Da mature protein is proteolytically processed in yeast and in planta, most likely resulting in the production of a heterodimer derived from a 30000 Da and a 17000 Da polypeptide Arabidopsis sp.

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis sp.

Storage Stability

Storage Stability Organism
-20°C, stable at -20°C glycerol for several months without loss of activity Arabidopsis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-O-caffeoyl-beta-D-glucose + choline
-
Arabidopsis sp. beta-D-glucose + caffeoylcholine
-
?
1-O-feruloyl-beta-D-glucose + choline
-
Arabidopsis sp. beta-D-glucose + feruloylcholine
-
?
1-O-sinapoyl-beta-D-glucose + 2-methylaminoethanol
-
Arabidopsis sp. beta-D-glucose + sinapoyl-2-methylaminoethanol
-
?
1-O-sinapoyl-beta-D-glucose + choline enzyme functions in sinapate ester synthesis Arabidopsis sp. D-glucose + sinapoylcholine
-
?
1-O-sinapoyl-beta-D-glucose + choline
-
Arabidopsis sp. beta-D-glucose + sinapoylcholine
-
?
1-O-sinapoyl-beta-D-glucose + N,N-dimethylethanolamine
-
Arabidopsis sp. beta-D-glucose + sinapoyl-N,N-dimethylethanolamine
-
?
1-O-sinapoyl-beta-D-glucose + neopentyl alcohol
-
Arabidopsis sp. beta-D-glucose + neopentylsinapate
-
?
1-O-sinapoyl-beta-D-glucose + Tris
-
Arabidopsis sp. ?
-
?
p-coumaroyl-beta-D-glucose + choline
-
Arabidopsis sp. beta-D-glucose + coumaroylcholine
-
?

Synonyms

Synonyms Comment Organism
SCT
-
Arabidopsis sp.
sinapoylglucose:choline sinapoyltransferase
-
Arabidopsis sp.