Cloned (Comment) | Organism |
---|---|
expression in a Saccharomyces cerevisiae expression system that lacks the carboxypeptodase Y gene and is defective in vacuolar targeting, resulting in secretion of heterologously expressed SCT | Arabidopsis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
1-O-caffeoyl-beta-D-glucose | pH 7.0, 30°C | Arabidopsis sp. | |
0.16 | - |
1-O-sinapoyl-beta-D-glucose | pH 7.0, 30°C | Arabidopsis sp. | |
0.41 | - |
1-O-feruloyl-beta-D-glucose | pH 7.0, 30°C | Arabidopsis sp. | |
1.9 | - |
p-coumaroyl-beta-D-glucose | pH 7.0, 30°C | Arabidopsis sp. | |
3.2 | - |
choline | pH 7.0, 30°C | Arabidopsis sp. | |
19 | - |
N,N-dimethylethanolamine | pH 7.0, 30°C | Arabidopsis sp. | |
25 | - |
neopentyl alcohol | pH 7.0, 30°C | Arabidopsis sp. | |
105 | - |
Tris | pH 7.0, 30°C | Arabidopsis sp. | |
245 | - |
2-Methylaminoethanol | pH 7.0, 30°C | Arabidopsis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-sinapoyl-beta-D-glucose + choline | Arabidopsis sp. | enzyme functions in sinapate ester synthesis | D-glucose + sinapoylcholine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis sp. | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the 50000 Da mature protein is proteolytically processed in yeast and in planta, most likely resulting in the production of a heterodimer derived from a 30000 Da and a 17000 Da polypeptide | Arabidopsis sp. |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis sp. |
Storage Stability | Organism |
---|---|
-20°C, stable at -20°C glycerol for several months without loss of activity | Arabidopsis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-caffeoyl-beta-D-glucose + choline | - |
Arabidopsis sp. | beta-D-glucose + caffeoylcholine | - |
? | |
1-O-feruloyl-beta-D-glucose + choline | - |
Arabidopsis sp. | beta-D-glucose + feruloylcholine | - |
? | |
1-O-sinapoyl-beta-D-glucose + 2-methylaminoethanol | - |
Arabidopsis sp. | beta-D-glucose + sinapoyl-2-methylaminoethanol | - |
? | |
1-O-sinapoyl-beta-D-glucose + choline | enzyme functions in sinapate ester synthesis | Arabidopsis sp. | D-glucose + sinapoylcholine | - |
? | |
1-O-sinapoyl-beta-D-glucose + choline | - |
Arabidopsis sp. | beta-D-glucose + sinapoylcholine | - |
? | |
1-O-sinapoyl-beta-D-glucose + N,N-dimethylethanolamine | - |
Arabidopsis sp. | beta-D-glucose + sinapoyl-N,N-dimethylethanolamine | - |
? | |
1-O-sinapoyl-beta-D-glucose + neopentyl alcohol | - |
Arabidopsis sp. | beta-D-glucose + neopentylsinapate | - |
? | |
1-O-sinapoyl-beta-D-glucose + Tris | - |
Arabidopsis sp. | ? | - |
? | |
p-coumaroyl-beta-D-glucose + choline | - |
Arabidopsis sp. | beta-D-glucose + coumaroylcholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCT | - |
Arabidopsis sp. |
sinapoylglucose:choline sinapoyltransferase | - |
Arabidopsis sp. |