BRENDA - Enzyme Database
show all sequences of 2.3.1.90

Enzymatic synthesis of 1,6-digalloylglucose from beta-glucogallin by beta-glucogallin:beta-glucogallin 6-0-galloyltransferase from oak leaves

Schmidt, S.W.; Denzel, K.; Schilling, G.; Gross, G.G.; Z. Naturforsch. C 42, 87-92 (1987)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
dilution destabilizes
Quercus robur
glycerol, 10%, stabilizes
Quercus robur
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
above 50 mM
Quercus robur
dithioerythritol
above 20 mM
Quercus robur
additional information
no inhibition by metal ions
Quercus robur
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
normal Michaelis-Menten kinetics; substrate saturation not reached at 20 mM
Quercus robur
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ion requirement
Quercus robur
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
400000
-
gel filtration
Quercus robur
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose
Quercus robur
first intermediate in the gallotannin biosynthesis in higher plants, serving as acyl donor and acceptor
1,6-di-O-galloyl-beta-D-glucose + D-glucose
-
Quercus robur
?
Organism
Organism
UniProt
Commentary
Textmining
Quercus robur
-
oak
-
Purification (Commentary)
Purification (Commentary)
Organism
partial purification with ammonium sulfate precipitation, DEAE-cellulose chromatography and gel filtration
Quercus robur
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
2-3 months old
Quercus robur
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0743
-
-
Quercus robur
Storage Stability
Storage Stability
Organism
-20C, stable in the presence of 10% glycerol
Quercus robur
2-4C, t1/2: 2 days, dilute preparation
Quercus robur
2-4C, t1/2: 7 days, concentrated preparation
Quercus robur
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose
-
487707
Quercus robur
1,6-di-O-galloyl-beta-D-glucose + D-glucose
-
487707
Quercus robur
?
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose
first intermediate in the gallotannin biosynthesis in higher plants, serving as acyl donor and acceptor
487707
Quercus robur
1,6-di-O-galloyl-beta-D-glucose + D-glucose
-
487707
Quercus robur
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
additional information
-
25% residual activity at 0C
Quercus robur
30
-
-
Quercus robur
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
highest stability at pH 4.5-6.5
Quercus robur
55
60
denaturation
Quercus robur
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
-
Quercus robur
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.5
about half-maximal activity at pH 5.5 and pH 7.5
Quercus robur
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4.5
6.5
highest stability at 30C
Quercus robur
General Stability (protein specific)
General Stability
Organism
dilution destabilizes
Quercus robur
glycerol, 10%, stabilizes
Quercus robur
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
above 50 mM
Quercus robur
dithioerythritol
above 20 mM
Quercus robur
additional information
no inhibition by metal ions
Quercus robur
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
normal Michaelis-Menten kinetics; substrate saturation not reached at 20 mM
Quercus robur
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ion requirement
Quercus robur
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
400000
-
gel filtration
Quercus robur
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose
Quercus robur
first intermediate in the gallotannin biosynthesis in higher plants, serving as acyl donor and acceptor
1,6-di-O-galloyl-beta-D-glucose + D-glucose
-
Quercus robur
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial purification with ammonium sulfate precipitation, DEAE-cellulose chromatography and gel filtration
Quercus robur
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
2-3 months old
Quercus robur
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0743
-
-
Quercus robur
Storage Stability (protein specific)
Storage Stability
Organism
-20C, stable in the presence of 10% glycerol
Quercus robur
2-4C, t1/2: 2 days, dilute preparation
Quercus robur
2-4C, t1/2: 7 days, concentrated preparation
Quercus robur
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose
-
487707
Quercus robur
1,6-di-O-galloyl-beta-D-glucose + D-glucose
-
487707
Quercus robur
?
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose
first intermediate in the gallotannin biosynthesis in higher plants, serving as acyl donor and acceptor
487707
Quercus robur
1,6-di-O-galloyl-beta-D-glucose + D-glucose
-
487707
Quercus robur
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
additional information
-
25% residual activity at 0C
Quercus robur
30
-
-
Quercus robur
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
highest stability at pH 4.5-6.5
Quercus robur
55
60
denaturation
Quercus robur
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
-
Quercus robur
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.5
about half-maximal activity at pH 5.5 and pH 7.5
Quercus robur
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4.5
6.5
highest stability at 30C
Quercus robur
Other publictions for EC 2.3.1.90
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
487705
Gross
-
Enzymic synthesis of di-O-phen ...
Quercus robur
Z. Naturforsch. C
45
37-41
1990
-
-
-
-
-
-
-
5
-
-
-
1
-
1
-
-
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
1
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487707
Schmidt
-
Enzymatic synthesis of 1,6-dig ...
Quercus robur
Z. Naturforsch. C
42
87-92
1987
-
-
-
-
-
2
3
1
-
1
1
1
-
1
-
-
1
-
-
1
1
3
2
-
-
2
-
2
-
1
1
1
-
-
-
-
-
-
-
-
-
-
2
-
3
-
1
-
1
1
1
-
-
-
1
-
1
1
3
2
-
2
-
2
-
1
1
1
-
-
-
-
-
-
-
487706
Gross
-
Synthesis of mono-, di- and tr ...
Quercus robur
Z. Naturforsch. C
38
519-523
1983
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
1
-
-
3
-
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
1
-
-
3
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-