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Literature summary for 2.3.1.87 extracted from
- Identification and characterization of two arylalkylamine N-acetyltransferases in the yellow fever mosquito, Aedes aegypti (2011), Insect Biochem. Mol. Biol., 41, 707-714.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| aaNAT1-4, aaNAT5b, aaNAT7, aaNAT9, and aaNAT11, homology sequence analysis, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression profiling of aaNAT1 aaNAT2 based on real time quantitative reverse transcriptase PCR, individual recombinant expression | Aedes aegypti |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetic analysis | Aedes aegypti | |
| 0.13 | - |
octopamine | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti |  |
| 0.14 | - |
octopamine | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
| 0.16 | - |
tryptamine | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
| 0.16 | - |
norepinephrine | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
| 0.17 | - |
tyramine | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
| 0.17 | - |
tryptamine | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti | |
| 0.17 | - |
norepinephrine | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti | |
| 0.18 | - |
methoxytryptamine | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
| 0.19 | - |
dopamine | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
| 0.23 | - |
serotonin | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
| 0.23 | - |
tyramine | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti | |
| 0.23 | - |
methoxytryptamine | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti | |
| 0.27 | - |
dopamine | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti | |
| 0.42 | - |
serotonin | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + dopamine | Aedes aegypti | - |
CoA + N-acetyldopamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aedes aegypti | - |
aaNAT1-4, aaNAT5b, aaNAT7, aaNAT9, and aaNAT11 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant aaNAT1-4, aaNAT5b, aaNAT7, aaNAT9, and aaNAT11 by chinin affinity and ion exchange chromatography, and gel filtration | Aedes aegypti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + dopamine | - |
Aedes aegypti | CoA + N-acetyldopamine | - |
? | |
| acetyl-CoA + methoxytryptamine | - |
Aedes aegypti | CoA + N-acetylmethoxytryptamine | - |
? | |
| acetyl-CoA + norepinephrine | - |
Aedes aegypti | CoA + N-acetylnorepinephrine | - |
? | |
| acetyl-CoA + octopamine | - |
Aedes aegypti | CoA + N-acetyloctopamine | - |
? | |
| acetyl-CoA + serotonin | i.e. 5-hydroxytryptamine | Aedes aegypti | CoA + N-acetylserotonin | - |
? | |
| acetyl-CoA + tryptamine | - |
Aedes aegypti | CoA + N-acetyltryptamine | - |
? | |
| acetyl-CoA + tyramine | - |
Aedes aegypti | CoA + N-acetyltyramine | - |
? | |
| additional information | substrate specificity, aaNAT1 and aaNAT2 show a broad substrate specificity and their affinity and catalytic efficiency to each of the seven arylalkylamines tested. The affinity between aaNAT1 and aaNAT2 to most of the arylalkylamines is similar except that aaNAT2 has less affinity than aaNAT1 to norepinephrine. Also aaNAT1 is more efficient in catalyzing all the tested substrates than that of aaNAT2 | Aedes aegypti | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 20000-35000, aaNAT1-4, aaNAT5b, aaNAT7, aaNAT9, and aaNAT11, SDS-PAGE | Aedes aegypti |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAT | - |
Aedes aegypti |
| PaaNAT | - |
Aedes aegypti |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Aedes aegypti |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.56 | - |
octopamine | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti | |
| 3.05 | - |
tryptamine | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti | |
| 3.16 | - |
tyramine | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti | |
| 3.17 | - |
norepinephrine | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti | |
| 3.47 | - |
serotonin | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti | |
| 3.65 | - |
dopamine | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti | |
| 3.74 | - |
methoxytryptamine | pH 7.3, 22°C, recombinant aaNAT2 | Aedes aegypti | |
| 6.775 | - |
tyramine | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
| 7.26 | - |
octopamine | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
| 7.3 | - |
dopamine | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
| 7.345 | - |
tryptamine | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
| 7.545 | - |
serotonin | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
| 7.69 | - |
methoxytryptamine | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
| 7.76 | - |
norepinephrine | pH 7.3, 22°C, recombinant aaNAT1 | Aedes aegypti | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
assay at | Aedes aegypti |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Aedes aegypti | |
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