Literature summary for 2.3.1.86 extracted from

Vance, D.E.; Mitsuhashi, O.; Bloch, K.
Purification and properties of the fatty acid synthetase from Mycobacterium phlei (1973), J. Biol. Chem., 248, 2303-2309.

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Activating Compound

Activating Compound	Comment	Organism	Structure
3-O-methylmannose	0.1 mM, lowers Km 4fold, activation by relieving product inhibition by binding long-chain acyl-CoA	Mycolicibacterium phlei
6-O-methylglucose	0.1 mM, lowers Km 4fold, activation by relieving product inhibition by binding long-chain acyl-CoA	Mycolicibacterium phlei

General Stability

General Stability	Organism
low ionic strength, 0.005 M, leads to dissociation into subunits, partially reactivated by increasing the ionic strength to 0.5 M	Mycolicibacterium phlei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0096	-	malonyl-CoA	in presence of polysaccharide	Mycolicibacterium phlei
0.04	-	malonyl-CoA	-	Mycolicibacterium phlei
0.09	-	acetyl-CoA	in presence of polysaccharide	Mycolicibacterium phlei
0.8	-	acetyl-CoA	-	Mycolicibacterium phlei

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1390000	-	sedimentation velocity data	Mycolicibacterium phlei

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium phlei	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
homogeneity	Mycolicibacterium phlei

Renatured (Commentary)

Renatured (Comment)	Organism
low concentration of phosphate buffer causes dissociation into inactive species, reaggregation and reactivation can be partially achieved by dialysis against 0.5 M phosphate buffer	Mycolicibacterium phlei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.375	-	-	Mycolicibacterium phlei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+	primers instead of acetyl-CoA: propionyl-CoA, butyryl-CoA or hexanoyl-CoA	Mycolicibacterium phlei	palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Mycolicibacterium phlei
NADPH	requirement	Mycolicibacterium phlei

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Release 2023.1 (January 2023)