Literature summary for 2.3.1.85 extracted from

Topolska, M.; Martinez-Montanes, F.; Ejsing, C.S.
Topolska, M.; Martixadnez-Montanes, F.; Ejsing, C. A simple and direct assay for monitoring fatty acid synthase activity and product-specificity by high-resolution mass spectrometry (2020), Biomolecules, 10, 118 .

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Application

Application	Comment	Organism
analysis	simple mass spectrometry-based assay that affords monitoring of FASN activity and its product specificity. Purified FASN is incubated with 13C-labeled malonyl-CoA, acetyl-CoA, and NADPH, at defined time points the reaction mixture is spiked with an internal non-esterified palmitic acid standard and extracted, and the extract is analyzed directly, without vacuum evaporation and chemical derivatization, by direct-infusion high-resolution mass spectrometry in negative ion mode. The assay supports essentially noise-free detection and absolute quantification of de novo synthetized 13C-labeled non-esterified fatty acids	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	Q71SP7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+	-	Bos taurus	palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O	-	?

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Release 2023.1 (January 2023)