General Stability | Organism |
---|---|
dithiothreitol stabilizes | Columba sp. |
foaming leads to surface inactivation | Columba sp. |
low ionic strength leads to dissociation of native enzyme and inactivation | Columba sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values for various partial activities of fatty acid synthetase | Columba sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Gallus gallus | - |
- |
soluble | - |
Rattus norvegicus | - |
- |
soluble | - |
Columba sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
450000 | - |
- |
Columba sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ | Gallus gallus | multifunctional enzyme, involved in animal fat synthesis | palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | - |
? | |
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ | Rattus norvegicus | multifunctional enzyme, involved in animal fat synthesis | palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | - |
? | |
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ | Columba sp. | multifunctional enzyme, involved in animal fat synthesis | palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Columba sp. | - |
pigeon | - |
Gallus gallus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Gallus gallus |
homogeneity | Rattus norvegicus |
homogeneity | Columba sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | reaction mechanism | Gallus gallus | |
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | reaction mechanism | Rattus norvegicus | |
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | reaction mechanism | Columba sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Columba sp. | - |
mammary gland | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.078 | 0.1 | palmitate | Columba sp. |
1.12 | 1.4 | NADPH, 30°C | Columba sp. |
Storage Stability | Organism |
---|---|
-20°C, at least a month in the presence of DTT | Columba sp. |
-20°C, under nitrogen at least 1-2 months | Columba sp. |
4°C, 90% loss of activity in 8 days with 40% dissociation of native enzyme, incubation at 38°C with 10 mM DTT restores activity | Columba sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ | - |
Gallus gallus | palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | in the absence of NADPH the product is triacetic acid lactone | ? | |
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ | - |
Rattus norvegicus | palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | in the absence of NADPH the product is triacetic acid lactone | ? | |
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ | specific for malonyl-CoA, acetyl-CoA can be replaced by propionyl-CoA or butyryl-CoA | Columba sp. | palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | in the absence of NADPH the product is triacetic acid lactone | ? | |
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ | multifunctional enzyme, involved in animal fat synthesis | Gallus gallus | palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | - |
? | |
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ | multifunctional enzyme, involved in animal fat synthesis | Rattus norvegicus | palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | - |
? | |
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ | multifunctional enzyme, involved in animal fat synthesis | Columba sp. | palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gallus gallus |
25 | - |
assay at | Rattus norvegicus |
25 | - |
assay at | Columba sp. |
30 | - |
assay at | Gallus gallus |
30 | - |
assay at | Rattus norvegicus |
30 | - |
assay at | Columba sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
50% loss of activity in dilute, mildly alkaline solution within 3.5 h, high ionic strength together with DTT reactivates | Columba sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Gallus gallus |
6.8 | - |
assay at | Rattus norvegicus |
6.8 | - |
assay at | Columba sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
inactivation, decreased by 0.2 M KCl or 0.02 mM NADP(H) | Columba sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4'-phosphopantetheine | - |
Gallus gallus | |
4'-phosphopantetheine | - |
Rattus norvegicus | |
4'-phosphopantetheine | - |
Columba sp. | |
additional information | no requirement for FMN | Gallus gallus | |
additional information | no requirement for FMN | Rattus norvegicus | |
additional information | no requirement for FMN | Columba sp. | |
NADH | requirement, 10% as effective as NADPH | Columba sp. | |
NADPH | - |
Gallus gallus | |
NADPH | - |
Rattus norvegicus | |
NADPH | - |
Columba sp. |