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Literature summary for 2.3.1.85 extracted from
- Physicochemical studies of the rat liver and adipose fatty acid synthetases (1979), J. Biol. Chem., 254, 7418-7426.
General Stability
| General Stability | Organism |
|---|---|
| phosphate buffer, 0.5 M, reassociates enzyme subunits | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Rattus norvegicus |
| additional information | - |
sedimentation coefficients of centrifugation of active enzyme | Rattus norvegicus |
| 244000 | - |
2 * 244000, ultracentrifugation in 6 M guanidinium chloride | Rattus norvegicus |
| 425000 | - |
sedimentation equilibrium method | Rattus norvegicus |
| 434000 | - |
ultracentrifugation in 0.5-0.8 M phosphate buffer | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Sprague-Dawley | - |
| Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | epididymal fat pads | Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity in liver and fat cells | Rattus norvegicus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 244000, ultracentrifugation in 6 M guanidinium chloride | Rattus norvegicus |
| More | alpha2-dimer of multifunctional subunits: each subunit contains up to seven active centres required for palmitate synthesis and an acyl carrier site, arranged in three domains but dimer is the only catalytically active form of fatty acid synthase | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 4'-phosphopantetheine | requirement, 1 mol associated with 1 mol subunit | Rattus norvegicus |  |
| 4'-phosphopantetheine | no covalently bound subunit component | Rattus norvegicus | |
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