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Literature summary for 2.3.1.82 extracted from

  • Kitao, T.; Miyoshi-Akiyama, T.; Kirikae, T.
    AAC(6)-Iaf, a novel aminoglycoside 6-N-acetyltransferase from multidrug-resistant Pseudomonas aeruginosa clinical isolates (2009), Antimicrob. Agents Chemother., 53, 2327-2334.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.82

Cloned(Commentary)

Cloned (Comment) Organism
into the vectors pSTV28 and pQE2, pQE2 is used for expression of the protein in Escherichia coli BL21DE3 pLysS cells Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 determined by SDS-PAGE and Western Blot analysis Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa C4TGI5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant AAC(6')-Iaf is purified using Ni-nitrilotriacetic acid agarose, native AAC(6')-Iaf from Pseudomonas aeruginosa on an anti-AAC(6')-Iaf-IgG coupled and NHS-activated Sepharose column Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + gentamicin
-
 Pseudomonas aeruginosa CoA + 6'-N-acetylgentamicin
-
 ?
acetyl-CoA + neomycin
-
 Pseudomonas aeruginosa CoA + N6'-acetylneomycin
-
 ?
additional information lividomycin A is also a substrate, although with extremely small activity, thus the enzyme has acetylation activity with alternate amino groups in aminoglycosides Pseudomonas aeruginosa ?
-
 ?

Synonyms

Synonyms Comment Organism
AAC(6')-Iaf
-
 Pseudomonas aeruginosa
aminoglycoside 6'-N-acetyltransferase
-
 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Pseudomonas aeruginosa