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Literature summary for 2.3.1.82 extracted from

  • Draker, K.A.; Northrop, D.B.; Wright, G.D.
    Kinetic mechanism of the GCN5-related chromosomal aminoglycoside acetyltransferase AAC(6')-Ii from Enterococcus faecium: evidence of dimer subunit cooperativity (2003), Biochemistry, 42, 6565-6574.
    View publication on PubMed
Search for more literature for 2.3.1.82

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Enterococcus faecium the enzyme is an important microbial resistance determinant ?
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + kanamycin A
-
 Enterococcus faecium CoA + N6'-acetylkanamycin A
-
 ?
acetyl-CoA + neamine
-
 Enterococcus faecium CoA + N6'-acetylneamine
-
 ?
acetyl-CoA + neomycin C
-
 Enterococcus faecium CoA + N6'-acetylneomycin
-
 ?
acetyl-CoA + ribostamycin
-
 Enterococcus faecium CoA + N6'acetylribostamycin
-
 ?
additional information the enzyme is an important microbial resistance determinant Enterococcus faecium ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Enterococcus faecium

Synonyms

Synonyms Comment Organism
AAC(6')-Ii
-
 Enterococcus faecium
aminoglycoside 6'-N-acetyltransferase
-
 Enterococcus faecium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.046
-
 ribostamycin pH 6.0, 37°C, mutant enzyme W164A Enterococcus faecium
0.048
-
 neamine pH 6.0, 37°C, mutant enzyme W164A Enterococcus faecium
0.08
-
 kanamycin A pH 6.0, 37°C, mutant enzyme W164A Enterococcus faecium
0.135
-
 neomycin C pH 6.0, 37°C, mutant enzyme W164A Enterococcus faecium
0.173
-
 acetyl-CoA pH 6.0, 37°C, mutant enzyme W164A Enterococcus faecium
0.205
-
 neomycin C pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
0.338
-
 ribostamycin pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
0.403
-
 acetyl-CoA pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
0.419
-
 neamine pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
0.816
-
 kanamycin A pH 6.0, 37°C, wild-type enzyme Enterococcus faecium