Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes F171L and Y80C | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
F171L | the mutant enzyme of enzyme variant Ib shows lower specific activity than the wild-type enzyme when either kanamycin or its semisynthetic derivative amikacin is used as substrate. Alteration of substrate specificity at 42°C. The acetylating activity for kanamycin is higher at 42°C than at 30°C, the ability to use amikacin as substrate is reduced at 42°C. Escherichia coli cells expressing the mutant enzyme are resistant the amikacin at 37°C but susceptible at 42°C | Klebsiella pneumoniae |
Y80C | the mutant enzyme shows only marginal levels of activity when either amikacin, kanamycin, tobramycin or netilmicin is used as substrate | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Klebsiella pneumoniae | involved in resistance to antibiotics | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | involved in resistance to antibiotics | Klebsiella pneumoniae | ? | - |
? |