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Literature summary for 2.3.1.82 extracted from

  • Lambert, T.; Ploy, M.C.; Courvalin, P.
    A spontaneous point mutation in the aac(6')-Ib‘ gene results in altered substrate specificity of aminoglycoside 6'-N-acetyltransferase of a Pseudomonas fluorescens strain (1994), FEMS Microbiol. Lett., 115, 297-304.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
aac(6')-Ib‘ gene Pseudomonas fluorescens

Protein Variants

Protein Variants Comment Organism
S83L the aac(6')-Ib‘ gene from strain BM2687 and the aac(6')-Ib gene from strain BM2656 show total identity with the exception of a C to T transition that results in a Ser to Leu substitution at position 83 of the deduced polypeptide. The enzyme encoded by aac(6')-Ib‘ shows resistance to gentamicin but not to amikacin. The enzyme encoded by aac(6')-Ib shows resistance to amikacin but not to gentamicin Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas fluorescens involved in resistance to antibiotics ?
-
?
additional information Pseudomonas fluorescens BM2687 involved in resistance to antibiotics ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
gene aac(6')-Ib'
-
Pseudomonas fluorescens BM2687
-
gene aac(6')-Ib'
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2'-N-ethylnetilmicin
-
Pseudomonas fluorescens CoA + N6'-acetyl-2'-N-ethylnetilmicin
-
?
acetyl-CoA + amikacin gentamicin complex: C1 41%, C1a 26% and C2 33%. The aac(6')-Ib‘ gene from strain BM2687 and the aac(6')-Ib gene from strain BM2656 show total identity with the exception of a C to T transition that results in a Ser to Leu substitution at position 83 of the deduced polypeptide. The enzyme encoded by aac(6')-Ib‘ shows resistance to gentamicin but not to amikacin. The enzyme encoded by aac(6')-Ib shows resistance to amikacin but not to gentamicin Pseudomonas fluorescens CoA + N6'-acetylamikacin
-
?
acetyl-CoA + amikacin gentamicin complex: C1 41%, C1a 26% and C2 33%. The aac(6')-Ib‘ gene from strain BM2687 and the aac(6')-Ib gene from strain BM2656 show total identity with the exception of a C to T transition that results in a Ser to Leu substitution at position 83 of the deduced polypeptide. The enzyme encoded by aac(6')-Ib‘ shows resistance to gentamicin but not to amikacin. The enzyme encoded by aac(6')-Ib shows resistance to amikacin but not to gentamicin Pseudomonas fluorescens BM2687 CoA + N6'-acetylamikacin
-
?
acetyl-CoA + gentamicin C1a gentamicin complex: C1 41%, C1a 26% and C2 33%. The aac(6')-Ib‘ gene from strain BM2687 and the aac(6')-Ib gene from strain BM2656 show total identity with the exception of a C to T transition that results in a Ser to Leu substitution at position 83 of the deduced polypeptide. The enzyme encoded by aac(6')-Ib‘ shows resistance to gentamicin but not to amikacin. The enzyme encoded by aac(6')-Ib shows resistance to amikacin but not to gentamicin Pseudomonas fluorescens CoA + N6'-acetylgentamicin C1a
-
?
acetyl-CoA + gentamicin C1a gentamicin complex: C1 41%, C1a 26% and C2 33%. The aac(6')-Ib‘ gene from strain BM2687 and the aac(6')-Ib gene from strain BM2656 show total identity with the exception of a C to T transition that results in a Ser to Leu substitution at position 83 of the deduced polypeptide. The enzyme encoded by aac(6')-Ib‘ shows resistance to gentamicin but not to amikacin. The enzyme encoded by aac(6')-Ib shows resistance to amikacin but not to gentamicin Pseudomonas fluorescens BM2687 CoA + N6'-acetylgentamicin C1a
-
?
acetyl-CoA + netilmicin
-
Pseudomonas fluorescens CoA + N6'-acetylnetilmicin
-
?
acetyl-CoA + netilmicin
-
Pseudomonas fluorescens BM2687 CoA + N6'-acetylnetilmicin
-
?
acetyl-CoA + tobramycin
-
Pseudomonas fluorescens CoA + N6'-acetyltobramycin
-
?
acetyl-CoA + tobramycin
-
Pseudomonas fluorescens BM2687 CoA + N6'-acetyltobramycin
-
?
additional information involved in resistance to antibiotics Pseudomonas fluorescens ?
-
?
additional information involved in resistance to antibiotics Pseudomonas fluorescens BM2687 ?
-
?