Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.82 extracted from

  • Wybenga-Groot, L.E.; Draker, K.a.; Wright, G.D.; Berghuis, A.M.
    Crystal structure of an aminoglycoside 6'-N-acetyltransferase: defining the GCN5-related N-acetyltransferase superfamily fold (1999), Structure, 7, 497-507.
    View publication on PubMed
Search for more literature for 2.3.1.82

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion technique, crystal structure of the 6'-N-acetyltransferase type li in complex with acetyl-CoA determined at 2.7 A resolution Enterococcus faecium

Inhibitors

Inhibitors Comment Organism Structure
poly-L-Lys substrate inhibition Enterococcus faecium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 poly-L-Lys pH 7.5, 37°C Enterococcus faecium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Enterococcus faecium involved in resistance to antibiotics ?
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecium Q47764 6'-N-acetyltransferase type li, transformation of Escherichia coli methionine auxotroph B834(DE3)/pLys S with a pPLaac-1 overexpression plasmid containing the aac(6')-li gene
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + histone a mixture of calf histones enriched in H3 and H4 Enterococcus faecium CoA + ?
-
 ?
acetyl-CoA + myelin basic protein
-
 Enterococcus faecium CoA + ?
-
 ?
acetyl-CoA + poly-L-Lys
-
 Enterococcus faecium CoA + ?
-
 ?
additional information no activity with aminoglycoside kinase APH(3')-IIIa, yeast homoserine dehydrogenase, spermine and serotonin Enterococcus faecium ?
-
 ?
additional information involved in resistance to antibiotics Enterococcus faecium ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0019
-
 poly-L-Lys pH 7.5, 37°C Enterococcus faecium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.78
-
 poly-L-Lys pH 7.5, 37°C Enterococcus faecium