BRENDA - Reference to 2.3.1.82; Id = 636903

isogenic plasmids pSCH4663 containing aac(6')-Ib gene and pSCHB4105 containing aac(6')-IIa gene, transferred to Escherichia coli DH5alpha

Escherichia coli

L119S

AAC(6')-Ib L119S. The AAC(6')-Ib protein is unable to efficiently modify gentamicin C1, 1.7% relative to sisomicin, however it is capable of modifying amikacin, 65.5% relative to sisomycin. The mutation results in a 2.8fold increase in acetylation of gentamicin C1, but causes an 8.7fold reduction in the ability to modify amikacin

Escherichia coli

S119L

AAC(6')-IIa S119L. The AAC(6')-IIa protein modifies gentamicin C1 at 10.1% relative to sisomicin, however it shows low activity towards amikacin, 4.1% relative to sisomycin. The mutation results in a 4.8fold reduction in the acetylation of gentamicin C1, but causes an 2fold increase in the ability to modify amikacin

Escherichia coli

additional information

Escherichia coli

involved in resistance to antibiotics

?

-

?

Escherichia coli

-

DH5alpha, AAC(6')-Ib protein and AAC(6')-IIa protein

-

acetyl-CoA + 2'-N-ethylnetilmicin

-

636903

Escherichia coli

CoA + N6'-acetyl-2'-N-ethylnetilmicin

-

?

acetyl-CoA + amikacin

the AAC(6')-Ib protein is unable to efficiently modify gentamicin C1, 1.7% relative activity to sisomicin, however it is capable of modifying amikacin, 65.5% relative activity to sisomycin. The mutant enzyme AAC(6')-Ib L119S shows a 2.8fold increase in acetylation of gentamicin C1, but a 8.7fold reduction in the ability to modify amikacin. The AAC(6')-IIa protein modifies gentamicin C1 at 10.1% relative activity to sisomicin, however it shows low activity towards amikacin, 4.1% activity relative to sisomycin. The mutation AAC(6')-IIa S119L results in a 4.8fold reduction in the acetylation of gentamicin C1, but causes an 2fold increase in the ability to modify amikacin

636903

Escherichia coli

CoA + N6'-acetylamikacin

-

?

acetyl-CoA + gentamicin C1

the AAC(6')-Ib protein is unable to efficiently modify gentamicin C1, 1.7% relative activity to sisomicin, however it is capable of modifying amikacin, 65.5% relative activity to sisomycin. The mutant enzyme AAC(6')-Ib L119S shows a 2.8fold increase in acetylation of gentamicin C1, but a 8.7fold reduction in the ability to modify amikacin. The AAC(6')-IIa protein modifies gentamicin C1 at 10.1% relative activity to sisomicin, however it shows low activity towards amikacin, 4.1% activity relative to sisomycin. The mutation AAC(6')-IIa S119L results in a 4.8fold reduction in the acetylation of gentamicin C1, but causes an 2fold increase in the ability to modify amikacin

636903

Escherichia coli

CoA + N6'-acetylgentamicin C1

-

?

acetyl-CoA + gentamicin C1a

poor substrate, AAC-(6')-II type

636903

Escherichia coli

CoA + N6'-acetylgentamicin C1a

-

?

acetyl-CoA + sisomicin

-

636903

Escherichia coli

CoA + N6'-acetylsisomicin

-

?

acetyl-CoA + tobramycin

-

636903

Escherichia coli

CoA + N6'-acetyltobramycin

-

?

additional information

a single amino acid, Leu119 in AAC(6')-Ib and S119 in AAC(6')-IIa, is largely responsible for determining the specificity of the AAC(6')-Ib and AAC(6')-IIa proteins. Changing this amino acid in either the AAC(6')-Ib or the AAC(6')-IIa protein results in a dramatic change in substrate specificity

636903

Escherichia coli

?

-

?

additional information

involved in resistance to antibiotics

636903

Escherichia coli

?

-

?

isogenic plasmids pSCH4663 containing aac(6')-Ib gene and pSCHB4105 containing aac(6')-IIa gene, transferred to Escherichia coli DH5alpha

Escherichia coli

L119S

AAC(6')-Ib L119S. The AAC(6')-Ib protein is unable to efficiently modify gentamicin C1, 1.7% relative to sisomicin, however it is capable of modifying amikacin, 65.5% relative to sisomycin. The mutation results in a 2.8fold increase in acetylation of gentamicin C1, but causes an 8.7fold reduction in the ability to modify amikacin

Escherichia coli

S119L

AAC(6')-IIa S119L. The AAC(6')-IIa protein modifies gentamicin C1 at 10.1% relative to sisomicin, however it shows low activity towards amikacin, 4.1% relative to sisomycin. The mutation results in a 4.8fold reduction in the acetylation of gentamicin C1, but causes an 2fold increase in the ability to modify amikacin

Escherichia coli

additional information

Escherichia coli

involved in resistance to antibiotics

?

-

?

acetyl-CoA + 2'-N-ethylnetilmicin

-

636903

Escherichia coli

CoA + N6'-acetyl-2'-N-ethylnetilmicin

-

?

acetyl-CoA + amikacin

the AAC(6')-Ib protein is unable to efficiently modify gentamicin C1, 1.7% relative activity to sisomicin, however it is capable of modifying amikacin, 65.5% relative activity to sisomycin. The mutant enzyme AAC(6')-Ib L119S shows a 2.8fold increase in acetylation of gentamicin C1, but a 8.7fold reduction in the ability to modify amikacin. The AAC(6')-IIa protein modifies gentamicin C1 at 10.1% relative activity to sisomicin, however it shows low activity towards amikacin, 4.1% activity relative to sisomycin. The mutation AAC(6')-IIa S119L results in a 4.8fold reduction in the acetylation of gentamicin C1, but causes an 2fold increase in the ability to modify amikacin

636903

Escherichia coli

CoA + N6'-acetylamikacin

-

?

acetyl-CoA + gentamicin C1

the AAC(6')-Ib protein is unable to efficiently modify gentamicin C1, 1.7% relative activity to sisomicin, however it is capable of modifying amikacin, 65.5% relative activity to sisomycin. The mutant enzyme AAC(6')-Ib L119S shows a 2.8fold increase in acetylation of gentamicin C1, but a 8.7fold reduction in the ability to modify amikacin. The AAC(6')-IIa protein modifies gentamicin C1 at 10.1% relative activity to sisomicin, however it shows low activity towards amikacin, 4.1% activity relative to sisomycin. The mutation AAC(6')-IIa S119L results in a 4.8fold reduction in the acetylation of gentamicin C1, but causes an 2fold increase in the ability to modify amikacin

636903

Escherichia coli

CoA + N6'-acetylgentamicin C1

-

?

acetyl-CoA + gentamicin C1a

poor substrate, AAC-(6')-II type

636903

Escherichia coli

CoA + N6'-acetylgentamicin C1a

-

?

acetyl-CoA + sisomicin

-

636903

Escherichia coli

CoA + N6'-acetylsisomicin

-

?

acetyl-CoA + tobramycin

-

636903

Escherichia coli

CoA + N6'-acetyltobramycin

-

?

additional information

a single amino acid, Leu119 in AAC(6')-Ib and S119 in AAC(6')-IIa, is largely responsible for determining the specificity of the AAC(6')-Ib and AAC(6')-IIa proteins. Changing this amino acid in either the AAC(6')-Ib or the AAC(6')-IIa protein results in a dramatic change in substrate specificity

636903

Escherichia coli

?

-

?

additional information

involved in resistance to antibiotics

636903

Escherichia coli

?

-

?

756795

Kim

Revisiting polymorphic divers ...

bacterium

Front. Microbiol.

9

1831

2018

-

14

-

1

-

1

-

756923

Chiem

Inhibition of aminoglycoside ...

Acinetobacter baumannii, Acinetobacter baumannii A155

Int. J. Antimicrob. Agents

51

271-273

2018

-

2

-

3

-

2

-

2

-

756924

Tran

Identification of a small mol ...

Acinetobacter baumannii, Klebsiella pneumoniae, Klebsiella pneumoniae JHCK1, Acinetobacter baumannii A155

Int. J. Antimicrob. Agents

51

752-761

2018

-

2

-

9

-

4

-

2

-

755691

Stogios

Structural and biochemical ch ...

Acinetobacter baumannii, Acinetobacter haemolyticus

ACS Infect. Dis.

3

132-143

2017

-

1

-

24

-

5

-

24

-

4

-

24

-

1

-

24

-

24

-

24

-

24

755693

Xu

Structural and functional sur ...

uncultured bacterium

ACS Infect. Dis.

3

653-665

2017

-

1

2

1

-

5

-

2

-

1

-

8

-

6

-

5

-

1

-

2

1

-

5

-

1

-

8

-

5

-

1

-

5

757594

Smith

Aminoglycoside resistance pro ...

Escherichia coli

Microb. Cell

4

402-410

2017

-

1

-

1

-

7

-

2

-

1

-

1

-

7

-

1

-

758025

Tabatabaei

A bifunctional aminoglycoside ...

Enterococcus faecalis, Enterococcus faecalis ATCC 700802

Plant Mol. Biol.

93

269-281

2017

-

1

-

3

-

3

-

1

-

757565

Chiem

Identification of an inhibito ...

Escherichia coli

MedChemComm

7

184-189

2016

-

1

-

4

-

2

-

2

-

2

1

-

757606

Tada

A novel 6'-N-aminoglycoside a ...

Serratia marcescens

Microb. Drug Resist.

22

103-108

2016

-

1

-

9

-

1

-

10

-

1

-

9

-

1

-

9

-

10

-

9

-

9

735446

Li

Inhibition of aminoglycoside a ...

Staphylococcus aureus, Pseudomonas aeruginosa

Antimicrob. Agents Chemother.

59

4148-4156

2015

-

4

-

11

-

4

-

4

-

5

-

5

4

-

4

-

735451

Chiem

Inhibition of aminoglycoside 6 ...

Klebsiella pneumoniae

Antimicrob. Agents Chemother.

59

5851-5853

2015

-

2

-

4

-

1

-

1

-

1

-

1

2

-

1

-

736322

Jin

New plasmid-mediated aminoglyc ...

Serratia marcescens, Serratia marcescens NUBL-11663

J. Antimicrob. Chemother.

70

1331-1337

2015

-

7

-

16

-

1

-

16

-

735363

Smith

Structure of the phosphotransf ...

Staphylococcus warneri

Acta Crystallogr. Sect. D

70

1561-1571

2014

-

1

-

1

-

2

-

1

-

1

-

735444

Lin

Inhibition of aminoglycoside 6 ...

Acinetobacter baumannii, Acinetobacter baumannii 155

Antimicrob. Agents Chemother.

58

4238-4241

2014

-

1

-

12

-

2

-

1

-

1

-

1

-

2

-

735445

Tada

Identification of a novel 6-N- ...

Stenotrophomonas maltophilia, Stenotrophomonas maltophilia IOMTU250

Antimicrob. Agents Chemother.

58

6324-6327

2014

-

9

-

2

-

15

-

2

-

9

-

9

-

15

-

9

-

1

-

9

735443

Tada

Novel 6'-N-aminoglycoside acet ...

Pseudomonas aeruginosa, Pseudomonas aeruginosa NCGM1588

Antimicrob. Agents Chemother.

57

96-100

2013

-

2

-

16

-

15

-

1

-

2

-

15

-

1

-

735730

Green

Domain dissection and characte ...

Serratia marcescens

Biochimie

95

1319-1325

2013

-

1

-

1

-

18

-

1

-

8

-

1

-

18

-

1

-

1

-

18

-

8

-

18

-

18

735759

Lin

Inhibitors of the aminoglycosi ...

Escherichia coli

Bioorg. Med. Chem. Lett.

23

5694-5698

2013

-

2

-

1

-

1

-

4

-

4

2

-

1

-

718594

Caldwell

Small-angle X-ray scattering a ...

Staphylococcus aureus

Antimicrob. Agents Chemother.

56

1899-1906

2012

-

1

-

1

-

1

-

1

-

1

-

719164

Kim

Identification of strain harbo ...

Escherichia coli, Klebsiella pneumoniae

BMB Rep.

44

262-266

2011

-

2

-

8

-

2

-

2

-

719603

Chowdhury

Transferable plasmid-mediated ...

Vibrio fluvialis

Int. J. Antimicrob. Agents

38

169-173

2011

-

1

-

16

-

1

-

701732

Kitao

AAC(6)-Iaf, a novel aminoglyco ...

Pseudomonas aeruginosa

Antimicrob. Agents Chemother.

53

2327-2334

2009

-

1

-

1

-

9

-

1

-

3

-

2

-

1

-

1

-

1

-

1

-

3

-

703215

Gao

Synthesis of a phosphonate-lin ...

Enterococcus faecium

Chemistry

15

2064-2070

2009

-

1

-

6

-

4

-

4

-

1

6

-

1

-

1

-

6

-

706524

Zaunbrecher

Overexpression of the chromoso ...

Mycobacterium tuberculosis

Proc. Natl. Acad. Sci. USA

106

20004-20009

2009

-

1

-

2

-

1

-

3

-

1

-

3

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

684411

Chu

Detection of broad-spectrum am ...

Enterococcus faecium

Anal. Biochem.

376

144-150

2008

-

6

-

3

-

5

-

6

-

6

-

5

-

6

-

685250

Magalhaes

Kinetic and structural analysi ...

Salmonella enterica

Biochemistry

47

579-584

2008

-

1

-

9

-

1

-

1

-

1

-

1

-

702249

Vetting

Mechanistic and structural ana ...

Escherichia coli

Biochemistry

47

9825-9835

2008

-

1

-

2

8

-

4

-

1

-

4

-

3

-

8

1

-

1

-

1

-

2

-

8

-

1

-

4

-

8

1

-

702549

Gao

Synthesis and use of sulfonami ...

Enterococcus faecium

Bioorg. Med. Chem. Lett.

18

5518-5522

2008

-

5

-

1

-

1

-

3

-

1

5

-

1

-

5

-

1

-

672253

Kim

Mechanistic characterization o ...

Pseudomonas aeruginosa

Biochemistry

46

5270-5282

2007

-

1

-

4

8

-

4

-

1

-

8

-

1

-

8

-

12

-

1

-

4

12

8

-

1

-

8

-

8

-

672563

Yan

The use of aminoglycoside deri ...

Enterococcus faecium

Bioorg. Med. Chem.

15

2944-2951

2007

-

1

-

1

-

1

-

1

-

672194

Kim

Characterization of the bifunc ...

Serratia marcescens

Biochemistry

45

8368-8377

2006

-

4

5

-

3

-

4

-

1

-

5

-

11

-

4

11

5

-

4

-

5

-

673770

Filipova

Occurrence of aminoglycoside-m ...

Enterococcus faecalis

Folia Microbiol. (Praha)

51

57-61

2006

-

1

-

675305

Gao

Synthesis and structure-activi ...

Enterococcus faecium

J. Med. Chem.

49

5273-5281

2006

-

15

-

1

-

1

-

15

-

15

-

1

-

657563

Pourreza

Mutagenesis analysis of a cons ...

Escherichia coli

Antimicrob. Agents Chemother.

49

2979-2982

2005

-

2

-

1

-

2

-

1

-

2

-

2

-

671064

Burk

Structures of aminoglycoside a ...

Enterococcus faecium

Acta Crystallogr. Sect. D

D61

1273-1279

2005

-

1

-

1

-

2

-

1

-

671333

Gao

Regio- and chemoselective 6-N- ...

Enterococcus faecium

Angew. Chem. Int. Ed. Engl.

44

6859-6862

2005

-

6

-

1

-

6

-

6

-

658066

Draker

Molecular mechanism of the ent ...

Enterococcus faecium

Biochemistry

43

446-454

2004

-

6

-

46

-

4

-

1

-

6

-

1

-

46

-

6

-

46

-

1

-

6

-

46

-

658426

Vetting

A bacterial acetyltransferase ...

Salmonella enterica

Chem. Biol.

11

565-573

2004

-

1

-

1

-

8

-

1

-

3

-

1

-

1

-

1

-

1

-

3

-

659093

Hamano

Overexpression and characteriz ...

Streptomyces albulus, Streptomyces albulus IFO14147

J. Biochem.

136

517-524

2004

-

1

-

11

-

12

-

1

-

16

-

2

-

1

-

11

-

1

-

16

-

657553

Magnet

Aminoglycoside resistance resu ...

Pseudomonas aeruginosa

Antimicrob. Agents Chemother.

47

1577-1583

2003

-

1

-

4

-

2

-

1

-

657554

Dery

The aminoglycoside 6'-N-acetyl ...

Escherichia coli

Antimicrob. Agents Chemother.

47

2897-2902

2003

-

1

-

9

-

2

-

1

-

657555

Casin

Salmonella enterica serovar ty ...

Salmonella enterica

Antimicrob. Agents Chemother.

47

697-703

2003

-

8

-

1

-

658002

Draker

Kinetic mechanism of the GCN5- ...

Enterococcus faecium

Biochemistry

42

6565-6574

2003

-

2

-

1

-

6

-

5

1

2

-

10

-

2

-

1

-

5

1

-

10

-

658998

Li

Role of the acetyltransferase ...

Stenotrophomonas maltophilia

J. Antimicrob. Chemother.

51

803-811

2003

-

1

-

1

-

6

-

1

-

1

-

1

-

1

-

1

-

659193

Boehr

The molecular basis of the exp ...

Enterococcus sp.

J. Biol. Chem.

278

12873-12880

2003

-

4

-

1

20

-

1

-

5

-

2

-

23

1

-

4

-

1

-

20

-

5

-

23

1

-

636912

Hegde

Thermodynamics of aminoglycosi ...

Salmonella enterica

Biochemistry

41

7519-7527

2002

-

1

-

1

-

6

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

2

-

636913

Magnet

Kinetic and mutagenic characte ...

Salmonella enterica

Biochemistry

40

3700-3709

2001

-

1

-

1

-

3

24

-

4

1

-

6

-

14

3

-

1

-

1

-

3

-

24

-

4

1

-

14

3

-

636914

Shmara

Systematic analysis of a conse ...

Klebsiella pneumoniae

Antimicrob. Agents Chemother.

45

3287-3292

2001

-

18

-

19

-

19

-

38

-

18

-

19

-

38

-

636904

Wybenga-Groot

Crystal structure of an aminog ...

Enterococcus faecium

Structure

7

497-507

1999

-

1

-

1

-

1

-

7

-

5

-

1

-

1

-

1

-

1

-

1

-

5

-

1

-

636908

Zhu

Role of aminoglycoside 6'-acet ...

Actinomyces sp., Actinomyces sp. 8

J. Antibiot.

52

889-894

1999

-

1

-

2

-

5

-

11

-

1

-

2

-

11

-

636909

Chavideh

Effects of F171 mutations in t ...

Klebsiella pneumoniae

Antimicrob. Agents Chemother.

43

2811-2812

1999

-

8

-

1

-

1

-

5

-

8

-

1

-

5

-

636907

Casin

Aminoglycoside 6'-N-acetyltran ...

Enterobacter cloacae, Citrobacter freundii, Enterobacter cloacae EC 1562

Antimicrob. Agents Chemother.

42

209-215

1998

-

3

-

14

-

6

-

3

-

6

-

636911

Panaite

Characterization of mutants of ...

Klebsiella pneumoniae

Plasmid

39

123-133

1998

-

1

-

2

-

1

-

14

-

1

-

1

-

2

-

1

-

1

-

636910

Wright

Overexpression and characteriz ...

Enterococcus faecium

Antimicrob. Agents Chemother.

41

956-960

1997

-

1

-

1

-

7

14

4

-

1

-

9

-

1

-

16

-

14

-

6

-

1

-

1

-

7

6

14

4

-

1

-

1

-

16

-

14

-

636905

Culebras

pH Modulation of aminoglycosid ...

Staphylococcus epidermidis, Staphylococcus epidermidis RYC 13036

J. Antimicrob. Chemother.

37

881-889

1996

-

4

11

-

2

-

2

-

11

-

2

-

12

-

4

12

11

-

2

-

11

-

2

-

636918

Bunny K.L.; Hall R.M.; Stokes H.W.

New mobile gene cassettes cont ...

Klebsiella aerogenes

Antimicrob. Agents Chemother.

39

686-693

1995

-

1

-

3

-

1

-

1

-

1

-

636906

Lambert

A spontaneous point mutation i ...

Pseudomonas fluorescens, Pseudomonas fluorescens BM2687

FEMS Microbiol. Lett.

115

297-304

1994

-

1

-

1

-

2

-

11

-

11

-

1

-

1

-

2

-

11

-

636903

Rather

Genetic analysis of bacterial ...

Escherichia coli

J. Bacteriol.

174

3196-3203

1992

-

1

-

2

-

1

-

1

-

8

-

1

-

2

-

1

-

8

-

636916

Tolmasky M.E.

Sequencing and expression of a ...

Klebsiella pneumoniae

Plasmid

24

218-226

1990

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

636915

Nobuta K.; Tolmasky M.E.; Crosa L.M.; Crosa J.H.

Sequencing and expression of t ...

Klebsiella pneumoniae

J. Bacteriol.

170

3769-3773

1988

-

1

-

1

-

1

-

1

-

1

-

636917

Tenover F.C.; Filpula D.; Phillips K.L.; Plorde J.J.

Cloning and sequencing of a ge ...

Citrobacter koseri

J. Bacteriol.

170

471-473

1988

-

1

-

1

-

5

-

1

-

1

-

1

-

1

-

636919

Van Nhieu G.T.; Collatz E.

Primary structure of an aminog ...

Serratia marcescens

J. Bacteriol.

169

5708-5714

1987

-

1

-

1

-

1

-

1

-

1

-

636901

Meyer

Characterization of aminoglyco ...

Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, Streptomyces kanamyceticus, Escherichia coli NR79, Serratia marcescens VU12944/77

J. Antimicrob. Chemother.

15

271-282

1985

-

2

-

4

-

4

6

-

11

-

4

-

33

4

-

5

-

2

-

4

-

4

6

-

4

-

33

4

-

5

-

636902

Martel

Kinetic studies of aminoglycos ...

Staphylococcus aureus

Eur. J. Biochem.

133

515-521

1983

-

1

6

-

1

-

1

-

1

-

1

-

6

-

1

-

4

-

1

-

6

4

-

1

-

1

-

1

-

6

-

1

-

636899

Le Goffic

Resistance to aminosides induc ...

Escherichia coli, Moraxella sp., Escherichia coli CS2R2

Biochimie

56

893-897

1974

-

1

5

-

3

-

3

-

1

-

1

24

-

2

-

1

-

5

-

3

-

1

-

1

24

-

2

-

487272

Benveniste

Aminoglycoside antibiotic-inac ...

Streptomyces kanamyceticus

Proc. Natl. Acad. Sci. USA

70

2276-2280

1973

-

1

-

1

-

2

-

1

-

1

-

2

-

1

-

636898

Benveniste

Enzymatic acetylation of amino ...

Escherichia coli

Biochemistry

10

1787-1796

1971

-

2

6

-

1

-

2

-

1

-

1

-

1

18

-

2

-

2

1

-

2

-

6

-

1

-

2

-

1

-

1

18

-

2

-

2

1

-

Genetic analysis of bacterial acetyltransferases: identification of amino acids determining the specificities of the aminoglycoside 6-N-acetyltransferase Ib and IIa proteins

Data extracted from this reference: