BRENDA - Enzyme Database
show all sequences of 2.3.1.81

Exploring the substrate promiscuity of drug-modifying enzymes for the chemoenzymatic generation of N-acylated aminoglycosides

Green, K.D.; Chen, W.; Houghton, J.L.; Fridman, M.; Garneau-Tsodikova, S.; ChemBioChem 11, 119-126 (2010)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
development of a methodology that utilizes aminoglycoside acetyltransferase and unnatural acyl-CoA analogues for the chemoenzymatic generation of regioselectively N-acylatedaminoglycoside analogues. Aminoglycosides are broad-spectrum antibiotics commonly used for the treatment of serious bacterial infections
Escherichia coli
Cloned(Commentary)
Commentary
Organism
expression of C- or N-terminally His-tagged enzyme from vectors pET22b, and pET28a or Int-pET19b-pps, respectively
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00057
-
sisomicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.00096
-
amikacin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0015
-
sisomicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.002
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
0.0021
-
amikacin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0027
-
tobramycin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0029
-
gentamicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0031
-
neomycin B
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0033
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.0046
-
gentamicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0048
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
0.0054
-
tobramycin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0055
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0091
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.0099
-
kanamycin A
pH 6.6, with acetyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.0111
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0135
-
kanamycin A
pH 6.6, with n-propionyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.0148
-
neomycin B
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0189
-
gentamicin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0716
-
amikacin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-bromo-thiophene-2-carbonyl-S-CoA + amikacin
-
703205
Escherichia coli
?
-
-
-
?
4-bromo-thiophene-2-carbonyl-S-CoA + gentamicin
-
703205
Escherichia coli
?
-
-
-
?
4-bromo-thiophene-2-carbonyl-S-CoA + neomycin B
-
703205
Escherichia coli
?
-
-
-
?
4-bromo-thiophene-2-carbonyl-S-CoA + paromomycin
-
703205
Escherichia coli
?
-
-
-
?
4-bromo-thiophene-2-carbonyl-S-CoA + tobramycin
-
703205
Escherichia coli
?
-
-
-
?
5-bromo-thiophene-2-carbonyl-S-CoA + kanamycin A
-
703205
Escherichia coli
?
-
-
-
?
5-bromo-thiophene-2-carbonyl-S-CoA + neomycin B
-
703205
Escherichia coli
?
-
-
-
?
6-fluoropicolinyl-CoA + neomycin B
-
703205
Escherichia coli
?
-
-
-
?
acetyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-acetyl-amikacin
-
-
-
?
acetyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-acetyl-gentamicin
-
-
-
?
acetyl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-acetyl-kanamycin A
-
-
-
?
acetyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-acetyl-neomycin B
-
-
-
?
acetyl-CoA + paromomycin
-
703205
Escherichia coli
CoA + N3'-acetyl-paromomycin
-
-
-
?
acetyl-CoA + sisomicin
-
703205
Escherichia coli
CoA + N3'-acetyl-sisomicin
-
-
-
?
acetyl-CoA + tobramycin
-
703205
Escherichia coli
CoA + N3'-acetyl-tobramycin
-
-
-
?
butyryl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-butyryl-kanamycin A
-
-
-
?
glutaryl-CoA + kanamycin A
-
703205
Escherichia coli
?
-
-
-
?
glycyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-glycyl-amikacin
-
-
-
?
glycyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-glycyl-gentamicin
-
-
-
?
glycyl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-glycyl-kanamycin A
-
-
-
?
glycyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-glycylneomycin B
-
-
-
?
glycyl-CoA + sisomicin
-
703205
Escherichia coli
CoA + N3'-glycyl-sisomicin
-
-
-
?
malonyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-malonyl-amikacin
-
-
-
?
malonyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-malonyl-gentamicin
-
-
-
?
malonyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-malonylneomycin B
-
-
-
?
methylmalonyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-methylmalonylneomycin B
-
-
-
?
additional information
no activity with benzoyl-CoA, acetoacetyl-CoA, crotonyl-CoA, isovaleryl-CoA, palmitoyl-CoA, and DL-3-hydroxy-butyryl-CoA by AAC(6')-APH(2'')
703205
Escherichia coli
?
-
-
-
-
N-propionyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-propionyl-amikacin
-
-
-
?
N-propionyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-propionyl-gentamicin
-
-
-
?
N-propionyl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-propionyl-kanamycin A
-
-
-
?
N-propionyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-propionylneomycin B
-
-
-
?
N-propionyl-CoA + paromomycin
-
703205
Escherichia coli
CoA + N3'-propionyl-paromomycin
-
-
-
?
N-propionyl-CoA + sisomicin
-
703205
Escherichia coli
CoA + N3'-propionyl-sisomicin
-
-
-
?
N-propionyl-CoA + tobramycin
-
703205
Escherichia coli
CoA + N3'-propionyl-tobramycin
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.012
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
0.017
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
0.041
-
gentamicin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.044
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.076
-
neomycin B
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.089
-
tobramycin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.121
-
sisomicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.123
-
gentamicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.124
-
amikacin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.126
-
amikacin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.134
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.144
-
kanamycin A
pH 6.6, with n-propionyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.146
-
amikacin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.163
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.173
-
sisomicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.179
-
tobramycin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.182
-
gentamicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.188
-
neomycin B
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.3
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.559
-
kanamycin A
pH 6.6, with acetyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
assay at
Escherichia coli
Application (protein specific)
Application
Commentary
Organism
synthesis
development of a methodology that utilizes aminoglycoside acetyltransferase and unnatural acyl-CoA analogues for the chemoenzymatic generation of regioselectively N-acylatedaminoglycoside analogues. Aminoglycosides are broad-spectrum antibiotics commonly used for the treatment of serious bacterial infections
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of C- or N-terminally His-tagged enzyme from vectors pET22b, and pET28a or Int-pET19b-pps, respectively
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00057
-
sisomicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.00096
-
amikacin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0015
-
sisomicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.002
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
0.0021
-
amikacin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0027
-
tobramycin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0029
-
gentamicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0031
-
neomycin B
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0033
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.0046
-
gentamicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0048
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
0.0054
-
tobramycin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0055
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0091
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.0099
-
kanamycin A
pH 6.6, with acetyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.0111
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0135
-
kanamycin A
pH 6.6, with n-propionyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.0148
-
neomycin B
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0189
-
gentamicin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.0716
-
amikacin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-bromo-thiophene-2-carbonyl-S-CoA + amikacin
-
703205
Escherichia coli
?
-
-
-
?
4-bromo-thiophene-2-carbonyl-S-CoA + gentamicin
-
703205
Escherichia coli
?
-
-
-
?
4-bromo-thiophene-2-carbonyl-S-CoA + neomycin B
-
703205
Escherichia coli
?
-
-
-
?
4-bromo-thiophene-2-carbonyl-S-CoA + paromomycin
-
703205
Escherichia coli
?
-
-
-
?
4-bromo-thiophene-2-carbonyl-S-CoA + tobramycin
-
703205
Escherichia coli
?
-
-
-
?
5-bromo-thiophene-2-carbonyl-S-CoA + kanamycin A
-
703205
Escherichia coli
?
-
-
-
?
5-bromo-thiophene-2-carbonyl-S-CoA + neomycin B
-
703205
Escherichia coli
?
-
-
-
?
6-fluoropicolinyl-CoA + neomycin B
-
703205
Escherichia coli
?
-
-
-
?
acetyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-acetyl-amikacin
-
-
-
?
acetyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-acetyl-gentamicin
-
-
-
?
acetyl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-acetyl-kanamycin A
-
-
-
?
acetyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-acetyl-neomycin B
-
-
-
?
acetyl-CoA + paromomycin
-
703205
Escherichia coli
CoA + N3'-acetyl-paromomycin
-
-
-
?
acetyl-CoA + sisomicin
-
703205
Escherichia coli
CoA + N3'-acetyl-sisomicin
-
-
-
?
acetyl-CoA + tobramycin
-
703205
Escherichia coli
CoA + N3'-acetyl-tobramycin
-
-
-
?
butyryl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-butyryl-kanamycin A
-
-
-
?
glutaryl-CoA + kanamycin A
-
703205
Escherichia coli
?
-
-
-
?
glycyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-glycyl-amikacin
-
-
-
?
glycyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-glycyl-gentamicin
-
-
-
?
glycyl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-glycyl-kanamycin A
-
-
-
?
glycyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-glycylneomycin B
-
-
-
?
glycyl-CoA + sisomicin
-
703205
Escherichia coli
CoA + N3'-glycyl-sisomicin
-
-
-
?
malonyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-malonyl-amikacin
-
-
-
?
malonyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-malonyl-gentamicin
-
-
-
?
malonyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-malonylneomycin B
-
-
-
?
methylmalonyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-methylmalonylneomycin B
-
-
-
?
additional information
no activity with benzoyl-CoA, acetoacetyl-CoA, crotonyl-CoA, isovaleryl-CoA, palmitoyl-CoA, and DL-3-hydroxy-butyryl-CoA by AAC(6')-APH(2'')
703205
Escherichia coli
?
-
-
-
-
N-propionyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-propionyl-amikacin
-
-
-
?
N-propionyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-propionyl-gentamicin
-
-
-
?
N-propionyl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-propionyl-kanamycin A
-
-
-
?
N-propionyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-propionylneomycin B
-
-
-
?
N-propionyl-CoA + paromomycin
-
703205
Escherichia coli
CoA + N3'-propionyl-paromomycin
-
-
-
?
N-propionyl-CoA + sisomicin
-
703205
Escherichia coli
CoA + N3'-propionyl-sisomicin
-
-
-
?
N-propionyl-CoA + tobramycin
-
703205
Escherichia coli
CoA + N3'-propionyl-tobramycin
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.012
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
0.017
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
0.041
-
gentamicin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.044
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.076
-
neomycin B
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.089
-
tobramycin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.121
-
sisomicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.123
-
gentamicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.124
-
amikacin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.126
-
amikacin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.134
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.144
-
kanamycin A
pH 6.6, with n-propionyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.146
-
amikacin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.163
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
0.173
-
sisomicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.179
-
tobramycin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.182
-
gentamicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.188
-
neomycin B
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.3
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
0.559
-
kanamycin A
pH 6.6, with acetyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
assay at
Escherichia coli
Other publictions for EC 2.3.1.81
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735448
Galimand
AAC(3)-XI, a new aminoglycosid ...
Corynebacterium striatum, Corynebacterium striatum BM4687
Antimicrob. Agents Chemother.
59
5647-5653
2015
-
-
1
-
-
-
1
4
-
-
-
6
-
10
-
-
1
-
-
1
-
-
18
-
1
-
-
3
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
7
-
-
-
6
-
-
-
1
-
1
-
-
18
-
1
-
-
6
1
-
-
-
-
2
2
-
3
6
736260
Atasoy
Modifying enzymes related amin ...
Acinetobacter baumannii
Int. J. Clin. Exp. Med.
8
2874-2880
2015
-
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
735678
Norris
Protein dynamics are influence ...
Pseudomonas aeruginosa
Biochemistry
53
30-38
2014
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
7
-
-
-
-
-
-
-
-
4
4
703205
Green
Exploring the substrate promis ...
Escherichia coli
ChemBioChem
11
119-126
2010
-
1
1
-
-
-
-
20
-
-
-
-
-
1
-
-
-
-
-
-
-
-
34
-
-
-
-
20
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
34
-
-
-
-
20
1
-
-
-
-
-
-
-
-
-
718870
Norris
Thermodynamics and kinetics of ...
Pseudomonas aeruginosa
Biochemistry
49
4027-4035
2010
-
-
1
-
-
-
5
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
-
-
-
8
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
5
2
8
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
8
-
-
-
-
-
-
-
-
8
8
718871
Norris
Interactions of coenzyme A wit ...
Pseudomonas aeruginosa
Biochemistry
49
4036-4042
2010
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
703467
Cabrera
Characterization of the enzyme ...
Salmonella enterica
Enferm. Infecc. Microbiol. Clin.
27
453-456
2009
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671371
Elbourne
Gene cassette encoding a 3-N-a ...
Saccharophagus degradans
Antimicrob. Agents Chemother.
50
2270-2271
2006
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657558
Levings
New integron-associated gene c ...
Salmonella enterica subsp. enterica serovar Kentucky
Antimicrob. Agents Chemother.
49
1238-1241
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672007
Magalhaes
The kinetic mechanism of AAC3- ...
Escherichia coli, Escherichia coli BL21(DE3) pLysS
Biochemistry
44
16275-16283
2005
-
-
1
-
-
-
3
15
-
-
4
-
-
3
-
-
1
-
-
-
-
-
20
1
-
-
-
15
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
3
-
15
-
-
4
-
-
-
-
1
-
-
-
-
20
1
-
-
-
15
1
-
-
1
-
-
-
-
-
-
672534
Welch
Discovery of non-carbohydrate ...
Homo sapiens
Bioorg. Med. Chem.
13
6252-6263
2005
-
-
1
-
-
-
3
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675934
Subba
The ribostamycin biosynthetic ...
Streptomyces ribosidificus
Mol. Cell
20
90-96
2005
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487553
Riccio
Novel 3-N-aminoglycoside acety ...
Pseudomonas aeruginosa
Antimicrob. Agents Chemother.
47
1746-1748
2003
-
-
1
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487552
Tsuchizaki
-
Rapid characterization by colo ...
Streptomyces griseus
Actinomycetologica
15
23-29
2001
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487551
Ishikawa
Identification and characteriz ...
Streptomyces griseus
Antimicrob. Agents Chemother.
44
437-440
2000
-
1
1
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487550
Wolf
Crystal structure of a GCN5-re ...
Serratia marcescens
Cell
94
439-449
1998
-
-
1
1
-
-
-
-
1
-
1
-
-
3
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637305
Gomez-Lus
Stability of dactimicin to ami ...
Escherichia coli
Drugs Exp. Clin. Res.
15
129-132
1989
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637304
Bongaerts
Effect of aminoglycoside conce ...
Escherichia coli, Escherichia coli JR88
Antimicrob. Agents Chemother.
32
740-746
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637300
Angelatou
Purification and properties of ...
Pseudomonas aeruginosa
J. Antibiot.
35
235-244
1982
-
-
1
-
-
2
3
1
-
3
1
-
-
3
-
-
1
-
-
-
1
2
11
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
2
-
3
-
1
-
3
1
-
-
-
-
1
-
-
1
2
11
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
661049
Coombe
Purification and properties of ...
Pseudomonas aeruginosa
Biochemistry
21
871-875
1982
-
-
-
-
-
1
2
1
-
-
1
-
-
2
-
-
1
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
1
-
-
1
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487549
Davies
Enzymatic modification of amin ...
Arizona sp., Escherichia coli, Klebsiella pneumoniae
Antimicrob. Agents Chemother.
14
69-72
1978
-
3
2
-
-
-
-
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
3
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637301
Williams
Kinetic mechanisms of gentamic ...
Escherichia coli, Escherichia coli C600
J. Biol. Chem.
253
5902-5907
1978
-
-
-
-
-
-
9
5
-
-
-
-
-
7
-
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
5
-
-
-
-
-
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
637299
Biddlecome
Enzymatic modification of amin ...
Escherichia coli, Escherichia coli R135/C600, Pseudomonas aeruginosa, Pseudomonas aeruginosa Travers PST
Antimicrob. Agents Chemother.
9
951-955
1976
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
22
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
22
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-