BRENDA - Enzyme Database
show all sequences of 2.3.1.81

The kinetic mechanism of AAC3-IV aminoglycoside acetyltransferase from Escherichia coli

Magalhaes, M.L.; Blanchard, J.S.; Biochemistry 44, 16275-16283 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in the pET-23a(+) expression vector
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
desulfo-CoA
-
Escherichia coli
netilmicin
-
Escherichia coli
ribostamycin
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00015
-
paromomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.00033
-
sisomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.00036
-
neomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.00067
-
apramycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.00097
-
gentamycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.00142
-
tobramycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.0031
-
ribostamycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.042
-
netilmicin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.086
-
acetyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.237
-
butirosin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.238
-
kanamycin A
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.477
-
malonyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.499
-
propionyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
1.16
-
butyryl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
11.07
-
amikacin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, enzyme in solution
Escherichia coli
54000
-
dynamic light scattering
Escherichia coli
55750
-
calculated from amino acid sequence
Escherichia coli
60000
-
Sephacryl-S200 gel filtration
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
Q306W4
-
-
Escherichia coli BL21(DE3) pLysS
Q306W4
-
-
Purification (Commentary)
Commentary
Organism
Fast Flow Q-Sepharose anion exchange column chromatography and Phenyl Sepharose column chromatography
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + amikacin
-
672007
Escherichia coli
CoA + N3'-acetylamikacin
-
-
-
?
acetyl-CoA + amikacin
-
672007
Escherichia coli BL21(DE3) pLysS
CoA + N3'-acetylamikacin
-
-
-
?
acetyl-CoA + apramycin
-
672007
Escherichia coli
CoA + N3'-acetylapramycin
-
-
-
?
acetyl-CoA + apramycin
-
672007
Escherichia coli BL21(DE3) pLysS
CoA + N3'-acetylapramycin
-
-
-
?
acetyl-CoA + butirosin
-
672007
Escherichia coli
CoA + N3'-acetylbutirosin
-
-
-
?
acetyl-CoA + gentamicin
-
672007
Escherichia coli
CoA + N3'-acetylgentamicin
-
-
-
?
acetyl-CoA + gentamicin
-
672007
Escherichia coli BL21(DE3) pLysS
CoA + N3'-acetylgentamicin
-
-
-
?
acetyl-CoA + kanamycin
-
672007
Escherichia coli
CoA + N3'-acetylkanamycin
-
-
-
?
acetyl-CoA + kanamycin
-
672007
Escherichia coli BL21(DE3) pLysS
CoA + N3'-acetylkanamycin
-
-
-
?
acetyl-CoA + neomycin
-
672007
Escherichia coli
CoA + ?
-
-
-
?
acetyl-CoA + netilmicin
-
672007
Escherichia coli
CoA + N3'-acetylnetilmicin
-
-
-
?
acetyl-CoA + paromomycin
-
672007
Escherichia coli
CoA + N3'-acetylparomomycin
-
-
-
?
acetyl-CoA + ribostamycin
-
672007
Escherichia coli
CoA + ?
-
-
-
?
acetyl-CoA + sisomycin
-
672007
Escherichia coli
CoA + N3'-acetylsisomycin
-
-
-
?
acetyl-CoA + tobramycin
-
672007
Escherichia coli
CoA + N3'-acetyltobramycin
-
-
-
?
acetyl-CoA + tobramycin
-
672007
Escherichia coli BL21(DE3) pLysS
CoA + N3'-acetyltobramycin
-
-
-
?
butyryl-CoA + ribostamycin
-
672007
Escherichia coli
CoA + ?
-
-
-
?
desulfo-CoA + ribostamycin
-
672007
Escherichia coli
?
-
-
-
?
malonyl-CoA + ribostamycin
-
672007
Escherichia coli
CoA + ?
-
-
-
?
propionyl-CoA + ribostamycin
-
672007
Escherichia coli
CoA + ?
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 28000, enzyme in solution
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.3
-
butyryl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.5
-
amikacin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
3.5
-
butirosin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
9
-
malonyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
15
-
paromomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
32
-
netilmicin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
33
-
propionyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
39
-
acetyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
40
-
gentamycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
51
-
sisomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
60
-
neomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
61
-
apramycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
80
-
kanamycin A
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
146
-
ribostamycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
162
-
tobramycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
corresponds to the plateau region of the pH profile, where the velocity is maximal and the variations in velocity due to changes in pH are smallest
Escherichia coli
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Escherichia coli
calculated from amino acid sequence
-
5.7
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in the pET-23a(+) expression vector
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
desulfo-CoA
-
Escherichia coli
netilmicin
-
Escherichia coli
ribostamycin
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00015
-
paromomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.00033
-
sisomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.00036
-
neomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.00067
-
apramycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.00097
-
gentamycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.00142
-
tobramycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.0031
-
ribostamycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.042
-
netilmicin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.086
-
acetyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.237
-
butirosin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.238
-
kanamycin A
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.477
-
malonyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.499
-
propionyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
1.16
-
butyryl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
11.07
-
amikacin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, enzyme in solution
Escherichia coli
54000
-
dynamic light scattering
Escherichia coli
55750
-
calculated from amino acid sequence
Escherichia coli
60000
-
Sephacryl-S200 gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
Fast Flow Q-Sepharose anion exchange column chromatography and Phenyl Sepharose column chromatography
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + amikacin
-
672007
Escherichia coli
CoA + N3'-acetylamikacin
-
-
-
?
acetyl-CoA + amikacin
-
672007
Escherichia coli BL21(DE3) pLysS
CoA + N3'-acetylamikacin
-
-
-
?
acetyl-CoA + apramycin
-
672007
Escherichia coli
CoA + N3'-acetylapramycin
-
-
-
?
acetyl-CoA + apramycin
-
672007
Escherichia coli BL21(DE3) pLysS
CoA + N3'-acetylapramycin
-
-
-
?
acetyl-CoA + butirosin
-
672007
Escherichia coli
CoA + N3'-acetylbutirosin
-
-
-
?
acetyl-CoA + gentamicin
-
672007
Escherichia coli
CoA + N3'-acetylgentamicin
-
-
-
?
acetyl-CoA + gentamicin
-
672007
Escherichia coli BL21(DE3) pLysS
CoA + N3'-acetylgentamicin
-
-
-
?
acetyl-CoA + kanamycin
-
672007
Escherichia coli
CoA + N3'-acetylkanamycin
-
-
-
?
acetyl-CoA + kanamycin
-
672007
Escherichia coli BL21(DE3) pLysS
CoA + N3'-acetylkanamycin
-
-
-
?
acetyl-CoA + neomycin
-
672007
Escherichia coli
CoA + ?
-
-
-
?
acetyl-CoA + netilmicin
-
672007
Escherichia coli
CoA + N3'-acetylnetilmicin
-
-
-
?
acetyl-CoA + paromomycin
-
672007
Escherichia coli
CoA + N3'-acetylparomomycin
-
-
-
?
acetyl-CoA + ribostamycin
-
672007
Escherichia coli
CoA + ?
-
-
-
?
acetyl-CoA + sisomycin
-
672007
Escherichia coli
CoA + N3'-acetylsisomycin
-
-
-
?
acetyl-CoA + tobramycin
-
672007
Escherichia coli
CoA + N3'-acetyltobramycin
-
-
-
?
acetyl-CoA + tobramycin
-
672007
Escherichia coli BL21(DE3) pLysS
CoA + N3'-acetyltobramycin
-
-
-
?
butyryl-CoA + ribostamycin
-
672007
Escherichia coli
CoA + ?
-
-
-
?
desulfo-CoA + ribostamycin
-
672007
Escherichia coli
?
-
-
-
?
malonyl-CoA + ribostamycin
-
672007
Escherichia coli
CoA + ?
-
-
-
?
propionyl-CoA + ribostamycin
-
672007
Escherichia coli
CoA + ?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 28000, enzyme in solution
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.3
-
butyryl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
0.5
-
amikacin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
3.5
-
butirosin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
9
-
malonyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
15
-
paromomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
32
-
netilmicin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
33
-
propionyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
39
-
acetyl-CoA
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
40
-
gentamycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
51
-
sisomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
60
-
neomycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
61
-
apramycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
80
-
kanamycin A
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
146
-
ribostamycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
162
-
tobramycin
in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
corresponds to the plateau region of the pH profile, where the velocity is maximal and the variations in velocity due to changes in pH are smallest
Escherichia coli
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Escherichia coli
calculated from amino acid sequence
-
5.7
Other publictions for EC 2.3.1.81
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735448
Galimand
AAC(3)-XI, a new aminoglycosid ...
Corynebacterium striatum, Corynebacterium striatum BM4687
Antimicrob. Agents Chemother.
59
5647-5653
2015
-
-
1
-
-
-
1
4
-
-
-
6
-
10
-
-
1
-
-
1
-
-
18
-
1
-
-
3
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
7
-
-
-
6
-
-
-
1
-
1
-
-
18
-
1
-
-
6
1
-
-
-
-
2
2
-
3
6
736260
Atasoy
Modifying enzymes related amin ...
Acinetobacter baumannii
Int. J. Clin. Exp. Med.
8
2874-2880
2015
-
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
735678
Norris
Protein dynamics are influence ...
Pseudomonas aeruginosa
Biochemistry
53
30-38
2014
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
7
-
-
-
-
-
-
-
-
4
4
703205
Green
Exploring the substrate promis ...
Escherichia coli
ChemBioChem
11
119-126
2010
-
1
1
-
-
-
-
20
-
-
-
-
-
1
-
-
-
-
-
-
-
-
34
-
-
-
-
20
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
34
-
-
-
-
20
1
-
-
-
-
-
-
-
-
-
718870
Norris
Thermodynamics and kinetics of ...
Pseudomonas aeruginosa
Biochemistry
49
4027-4035
2010
-
-
1
-
-
-
5
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
-
-
-
8
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
5
2
8
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
8
-
-
-
-
-
-
-
-
8
8
718871
Norris
Interactions of coenzyme A wit ...
Pseudomonas aeruginosa
Biochemistry
49
4036-4042
2010
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
703467
Cabrera
Characterization of the enzyme ...
Salmonella enterica
Enferm. Infecc. Microbiol. Clin.
27
453-456
2009
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671371
Elbourne
Gene cassette encoding a 3-N-a ...
Saccharophagus degradans
Antimicrob. Agents Chemother.
50
2270-2271
2006
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657558
Levings
New integron-associated gene c ...
Salmonella enterica subsp. enterica serovar Kentucky
Antimicrob. Agents Chemother.
49
1238-1241
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672007
Magalhaes
The kinetic mechanism of AAC3- ...
Escherichia coli, Escherichia coli BL21(DE3) pLysS
Biochemistry
44
16275-16283
2005
-
-
1
-
-
-
3
15
-
-
4
-
-
3
-
-
1
-
-
-
-
-
20
1
-
-
-
15
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
3
-
15
-
-
4
-
-
-
-
1
-
-
-
-
20
1
-
-
-
15
1
-
-
1
-
-
-
-
-
-
672534
Welch
Discovery of non-carbohydrate ...
Homo sapiens
Bioorg. Med. Chem.
13
6252-6263
2005
-
-
1
-
-
-
3
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675934
Subba
The ribostamycin biosynthetic ...
Streptomyces ribosidificus
Mol. Cell
20
90-96
2005
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487553
Riccio
Novel 3-N-aminoglycoside acety ...
Pseudomonas aeruginosa
Antimicrob. Agents Chemother.
47
1746-1748
2003
-
-
1
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487552
Tsuchizaki
-
Rapid characterization by colo ...
Streptomyces griseus
Actinomycetologica
15
23-29
2001
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487551
Ishikawa
Identification and characteriz ...
Streptomyces griseus
Antimicrob. Agents Chemother.
44
437-440
2000
-
1
1
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487550
Wolf
Crystal structure of a GCN5-re ...
Serratia marcescens
Cell
94
439-449
1998
-
-
1
1
-
-
-
-
1
-
1
-
-
3
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637305
Gomez-Lus
Stability of dactimicin to ami ...
Escherichia coli
Drugs Exp. Clin. Res.
15
129-132
1989
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637304
Bongaerts
Effect of aminoglycoside conce ...
Escherichia coli, Escherichia coli JR88
Antimicrob. Agents Chemother.
32
740-746
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637300
Angelatou
Purification and properties of ...
Pseudomonas aeruginosa
J. Antibiot.
35
235-244
1982
-
-
1
-
-
2
3
1
-
3
1
-
-
3
-
-
1
-
-
-
1
2
11
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
2
-
3
-
1
-
3
1
-
-
-
-
1
-
-
1
2
11
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
661049
Coombe
Purification and properties of ...
Pseudomonas aeruginosa
Biochemistry
21
871-875
1982
-
-
-
-
-
1
2
1
-
-
1
-
-
2
-
-
1
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
1
-
-
1
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487549
Davies
Enzymatic modification of amin ...
Arizona sp., Escherichia coli, Klebsiella pneumoniae
Antimicrob. Agents Chemother.
14
69-72
1978
-
3
2
-
-
-
-
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
3
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637301
Williams
Kinetic mechanisms of gentamic ...
Escherichia coli, Escherichia coli C600
J. Biol. Chem.
253
5902-5907
1978
-
-
-
-
-
-
9
5
-
-
-
-
-
7
-
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
5
-
-
-
-
-
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
637299
Biddlecome
Enzymatic modification of amin ...
Escherichia coli, Escherichia coli R135/C600, Pseudomonas aeruginosa, Pseudomonas aeruginosa Travers PST
Antimicrob. Agents Chemother.
9
951-955
1976
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
22
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
22
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-