BRENDA - Enzyme Database
show all sequences of 2.3.1.81

Purification and properties of two gentamicin-modifying enzymes, coded by a single plasmid pPK237 originating from Pseudomonas aeruginosa

Angelatou, F.; Litsas, S.B.; Kontomichalou, P.; J. Antibiot. 35, 235-244 (1982)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
Pseudomonas aeruginosa plasmid pPK237 transferred to and expressed in Escherichia coli K12
Pseudomonas aeruginosa
General Stability
General Stability
Organism
dialysis against EDTA with subsequent Mg2+-addition to dialyzed enzyme has no effect on activity
Pseudomonas aeruginosa
gel filtration leads to complete inactivation
Pseudomonas aeruginosa
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
above 0.5 mM
Pseudomonas aeruginosa
additional information
no inhibition by NH4+, K+, Na+, DTT, N-methylmaleimide
Pseudomonas aeruginosa
Zn2+
-
Pseudomonas aeruginosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.066
-
gentamicin C1
-
Pseudomonas aeruginosa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
1.0 mM, about 100% activity enhancement
Pseudomonas aeruginosa
Mg2+
1.5 mM, about 100% activity enhancement
Pseudomonas aeruginosa
NH4+
slight activation
Pseudomonas aeruginosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
amino acid analysis
Pseudomonas aeruginosa
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas aeruginosa
-
plasmid pPK237 transferred to and expressed in Escherichia coli K12
-
Purification (Commentary)
Purification (Commentary)
Organism
plasmid pPK237 transferred to and expressed in E. coli K12, affinity chromatography
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
34
-
-
Pseudomonas aeruginosa
Storage Stability
Storage Stability
Organism
-20C, partially purified, several months
Pseudomonas aeruginosa
-20C, purified, several days
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetyl-CoA + dibekacin
acetylation at 8% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3''-acetyldibekacin
-
-
-
?
acetyl-CoA + gentamicin A
acetylation at 6% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetylgentamicin A
-
-
-
?
acetyl-CoA + gentamicin C
acetylation at 86% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetylgentamicin C
-
-
-
?
acetyl-CoA + gentamicin C1
best substrate
637300
Pseudomonas aeruginosa
CoA + N3'-acetylgentamicin C1
-
-
-
?
acetyl-CoA + gentamicin C1a
acetylation at 60% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetylgentamicin C1a
-
637300
Pseudomonas aeruginosa
?
acetyl-CoA + gentamicin C2
acetylation at 84% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetylgentamicin C2
-
-
-
?
acetyl-CoA + kanamycin A
acetylation at 7% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetylkanamycin A
-
-
-
?
acetyl-CoA + sisomicin
acetylation at 51% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetylsisomicin
-
-
-
?
acetyl-CoA + tobramycin
acetylation at 6% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetyltobramycin
-
-
-
?
additional information
no substrates are amikacin
637300
Pseudomonas aeruginosa
?
-
-
-
-
additional information
lividomycin A, butirosin A and B
637300
Pseudomonas aeruginosa
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Pseudomonas aeruginosa
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
above, inactivation within 2 min
Pseudomonas aeruginosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.6
-
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
Pseudomonas aeruginosa plasmid pPK237 transferred to and expressed in Escherichia coli K12
Pseudomonas aeruginosa
General Stability (protein specific)
General Stability
Organism
dialysis against EDTA with subsequent Mg2+-addition to dialyzed enzyme has no effect on activity
Pseudomonas aeruginosa
gel filtration leads to complete inactivation
Pseudomonas aeruginosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
above 0.5 mM
Pseudomonas aeruginosa
additional information
no inhibition by NH4+, K+, Na+, DTT, N-methylmaleimide
Pseudomonas aeruginosa
Zn2+
-
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.066
-
gentamicin C1
-
Pseudomonas aeruginosa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
1.0 mM, about 100% activity enhancement
Pseudomonas aeruginosa
Mg2+
1.5 mM, about 100% activity enhancement
Pseudomonas aeruginosa
NH4+
slight activation
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
amino acid analysis
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
Commentary
Organism
plasmid pPK237 transferred to and expressed in E. coli K12, affinity chromatography
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
34
-
-
Pseudomonas aeruginosa
Storage Stability (protein specific)
Storage Stability
Organism
-20C, partially purified, several months
Pseudomonas aeruginosa
-20C, purified, several days
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetyl-CoA + dibekacin
acetylation at 8% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3''-acetyldibekacin
-
-
-
?
acetyl-CoA + gentamicin A
acetylation at 6% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetylgentamicin A
-
-
-
?
acetyl-CoA + gentamicin C
acetylation at 86% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetylgentamicin C
-
-
-
?
acetyl-CoA + gentamicin C1
best substrate
637300
Pseudomonas aeruginosa
CoA + N3'-acetylgentamicin C1
-
-
-
?
acetyl-CoA + gentamicin C1a
acetylation at 60% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetylgentamicin C1a
-
637300
Pseudomonas aeruginosa
?
acetyl-CoA + gentamicin C2
acetylation at 84% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetylgentamicin C2
-
-
-
?
acetyl-CoA + kanamycin A
acetylation at 7% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetylkanamycin A
-
-
-
?
acetyl-CoA + sisomicin
acetylation at 51% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetylsisomicin
-
-
-
?
acetyl-CoA + tobramycin
acetylation at 6% the rate of gentamicin C1
637300
Pseudomonas aeruginosa
CoA + N3'-acetyltobramycin
-
-
-
?
additional information
no substrates are amikacin
637300
Pseudomonas aeruginosa
?
-
-
-
-
additional information
lividomycin A, butirosin A and B
637300
Pseudomonas aeruginosa
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Pseudomonas aeruginosa
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
above, inactivation within 2 min
Pseudomonas aeruginosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.6
-
Pseudomonas aeruginosa
Other publictions for EC 2.3.1.81
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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-
18
-
3
1
-
-
3
1
-
-
1
-
-
-
-
-
1
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-
-
-
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1
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7
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-
-
6
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-
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1
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18
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1
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1
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1
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1
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1
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1
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735678
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-
-
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8
-
1
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7
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4
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8
-
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7
-
-
-
-
-
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-
-
4
4
703205
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-
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-
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34
-
4
-
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20
1
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1
1
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-
20
-
-
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-
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-
34
-
-
-
-
20
1
-
-
-
-
-
-
-
-
-
718870
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-
-
-
5
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
1
-
-
-
8
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
5
2
8
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
8
-
-
-
-
-
-
-
-
8
8
718871
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Pseudomonas aeruginosa
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-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
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5
-
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4
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4
-
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-
-
-
-
5
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
703467
Cabrera
Characterization of the enzyme ...
Salmonella enterica
Enferm. Infecc. Microbiol. Clin.
27
453-456
2009
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
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-
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1
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2
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1
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671371
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Gene cassette encoding a 3-N-a ...
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50
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-
-
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-
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1
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2
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657558
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49
1238-1241
2005
-
-
-
-
-
-
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3
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-
672007
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-
-
1
-
-
-
3
15
-
-
4
-
-
3
-
-
1
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20
1
3
-
-
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15
1
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1
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1
-
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3
-
15
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4
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1
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20
1
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15
1
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-
1
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672534
Welch
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1
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3
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1
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675934
Subba
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Streptomyces ribosidificus
Mol. Cell
20
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2005
-
-
1
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-
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-
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1
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3
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1
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1
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1
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487553
Riccio
Novel 3-N-aminoglycoside acety ...
Pseudomonas aeruginosa
Antimicrob. Agents Chemother.
47
1746-1748
2003
-
-
1
-
-
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-
1
-
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3
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10
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1
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1
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10
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487552
Tsuchizaki
-
Rapid characterization by colo ...
Streptomyces griseus
Actinomycetologica
15
23-29
2001
-
-
-
-
-
-
-
-
1
-
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-
1
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1
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487551
Ishikawa
Identification and characteriz ...
Streptomyces griseus
Antimicrob. Agents Chemother.
44
437-440
2000
-
1
1
-
-
-
-
-
2
-
-
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2
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1
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1
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1
1
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2
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1
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487550
Wolf
Crystal structure of a GCN5-re ...
Serratia marcescens
Cell
94
439-449
1998
-
-
1
1
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1
-
1
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3
-
1
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1
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1
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1
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1
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1
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1
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1
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1
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637305
Gomez-Lus
Stability of dactimicin to ami ...
Escherichia coli
Drugs Exp. Clin. Res.
15
129-132
1989
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Bongaerts
Effect of aminoglycoside conce ...
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1988
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1
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10
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-
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-
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-
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-
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-
-
-
-
-
-
-
-
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1
-
-
-
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10
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Angelatou
Purification and properties of ...
Pseudomonas aeruginosa
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1982
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1
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1
2
11
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1
-
1
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1
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-
-
-
-
-
-
-
1
-
-
-
2
-
3
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1
-
3
1
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-
-
1
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1
2
11
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1
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1
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1
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661049
Coombe
Purification and properties of ...
Pseudomonas aeruginosa
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1982
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1
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1
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1
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2
-
1
-
2
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-
-
-
-
-
-
-
-
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-
-
-
-
-
-
1
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2
-
1
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-
1
-
-
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-
1
-
-
2
-
1
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-
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487549
Davies
Enzymatic modification of amin ...
Arizona sp., Escherichia coli, Klebsiella pneumoniae
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69-72
1978
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3
2
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3
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3
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2
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3
2
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-
3
-
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3
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Williams
Kinetic mechanisms of gentamic ...
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6
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1
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9
-
5
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6
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1
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637299
Biddlecome
Enzymatic modification of amin ...
Escherichia coli, Escherichia coli R135/C600, Pseudomonas aeruginosa, Pseudomonas aeruginosa Travers PST
Antimicrob. Agents Chemother.
9
951-955
1976
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4
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22
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2
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22
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2
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