Enzymatic modification of aminoglycoside antibiotics: a new 3-N-acetylating enzyme from a Pseudomonas aeruginosa isolate

Biddlecome, S.; Haas, M.; Davies, J.; Rane, D.F.; Daniels, P.J.L.; Antimicrob. Agents Chemother. 9, 951-955 (1976)

Data extracted from this reference:

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

acetyl-CoA + gentamicin C1

-

637299

Escherichia coli

CoA + N3'-acetylgentamicin C1

-

-

-

?

acetyl-CoA + gentamicin C1

-

637299

Pseudomonas aeruginosa

CoA + N3'-acetylgentamicin C1

-

-

-

?

acetyl-CoA + gentamicin C1

-

637299

Pseudomonas aeruginosa Travers PST

CoA + N3'-acetylgentamicin C1

-

-

-

?

acetyl-CoA + gentamicin C1

-

637299

Escherichia coli R135/C600

CoA + N3'-acetylgentamicin C1

-

-

-

?

acetyl-CoA + kanamycin A

-

637299

Escherichia coli

CoA + N3'-acetylkanamycin A

-

-

-

?

acetyl-CoA + kanamycin A

-

637299

Pseudomonas aeruginosa

CoA + N3'-acetylkanamycin A

-

-

-

?

acetyl-CoA + kanamycin A

-

637299

Pseudomonas aeruginosa Travers PST

CoA + N3'-acetylkanamycin A

-

-

-

?

acetyl-CoA + kanamycin A

-

637299

Escherichia coli R135/C600

CoA + N3'-acetylkanamycin A

-

-

-

?

acetyl-CoA + neomycin B

-

637299

Escherichia coli

CoA + ?

-

-

-

?

acetyl-CoA + paromomycin

not E. coli

637299

Escherichia coli

CoA + N3'-acetylparomomycin B

-

-

-

?

acetyl-CoA + sisomicin

best substrate

637299

Escherichia coli

CoA + N3'-acetylsisomicin

-

637299

Escherichia coli

?

acetyl-CoA + sisomicin

best substrate

637299

Pseudomonas aeruginosa

CoA + N3'-acetylsisomicin

-

637299

Pseudomonas aeruginosa

?

acetyl-CoA + sisomicin

best substrate

637299

Pseudomonas aeruginosa Travers PST

CoA + N3'-acetylsisomicin

-

637299

Pseudomonas aeruginosa Travers PST

?

acetyl-CoA + sisomicin

best substrate

637299

Escherichia coli R135/C600

CoA + N3'-acetylsisomicin

-

637299

Escherichia coli R135/C600

?

acetyl-CoA + tobramycin

-

637299

Escherichia coli

CoA + N3'-acetyltobramycin

-

-

-

?

acetyl-CoA + tobramycin

-

637299

Pseudomonas aeruginosa

CoA + N3'-acetyltobramycin

-

-

-

?

acetyl-CoA + tobramycin

-

637299

Pseudomonas aeruginosa Travers PST

CoA + N3'-acetyltobramycin

-

-

-

?

acetyl-CoA + tobramycin

-

637299

Escherichia coli R135/C600

CoA + N3'-acetyltobramycin

-

-

-

?

additional information

no substrates are amikacin

637299

Escherichia coli

?

-

-

-

?

additional information

no substrates are amikacin

637299

Pseudomonas aeruginosa

?

-

-

-

?

additional information

no substrates are amikacin

637299

Pseudomonas aeruginosa Travers PST

?

-

-

-

?

additional information

no substrates are amikacin

637299

Escherichia coli R135/C600

?

-

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

acetyl-CoA + gentamicin C1

-

637299

Escherichia coli

CoA + N3'-acetylgentamicin C1

-

-

-

?

acetyl-CoA + gentamicin C1

-

637299

Pseudomonas aeruginosa

CoA + N3'-acetylgentamicin C1

-

-

-

?

acetyl-CoA + gentamicin C1

-

637299

Pseudomonas aeruginosa Travers PST

CoA + N3'-acetylgentamicin C1

-

-

-

?

acetyl-CoA + gentamicin C1

-

637299

Escherichia coli R135/C600

CoA + N3'-acetylgentamicin C1

-

-

-

?

acetyl-CoA + kanamycin A

-

637299

Escherichia coli

CoA + N3'-acetylkanamycin A

-

-

-

?

acetyl-CoA + kanamycin A

-

637299

Pseudomonas aeruginosa

CoA + N3'-acetylkanamycin A

-

-

-

?

acetyl-CoA + kanamycin A

-

637299

Pseudomonas aeruginosa Travers PST

CoA + N3'-acetylkanamycin A

-

-

-

?

acetyl-CoA + kanamycin A

-

637299

Escherichia coli R135/C600

CoA + N3'-acetylkanamycin A

-

-

-

?

acetyl-CoA + neomycin B

-

637299

Escherichia coli

CoA + ?

-

-

-

?

acetyl-CoA + paromomycin

not E. coli

637299

Escherichia coli

CoA + N3'-acetylparomomycin B

-

-

-

?

acetyl-CoA + sisomicin

best substrate

637299

Escherichia coli

CoA + N3'-acetylsisomicin

-

637299

Escherichia coli

?

acetyl-CoA + sisomicin

best substrate

637299

Pseudomonas aeruginosa

CoA + N3'-acetylsisomicin

-

637299

Pseudomonas aeruginosa

?

acetyl-CoA + sisomicin

best substrate

637299

Pseudomonas aeruginosa Travers PST

CoA + N3'-acetylsisomicin

-

637299

Pseudomonas aeruginosa Travers PST

?

acetyl-CoA + sisomicin

best substrate

637299

Escherichia coli R135/C600

CoA + N3'-acetylsisomicin

-

637299

Escherichia coli R135/C600

?

acetyl-CoA + tobramycin

-

637299

Escherichia coli

CoA + N3'-acetyltobramycin

-

-

-

?

acetyl-CoA + tobramycin

-

637299

Pseudomonas aeruginosa

CoA + N3'-acetyltobramycin

-

-

-

?

acetyl-CoA + tobramycin

-

637299

Pseudomonas aeruginosa Travers PST

CoA + N3'-acetyltobramycin

-

-

-

?

acetyl-CoA + tobramycin

-

637299

Escherichia coli R135/C600

CoA + N3'-acetyltobramycin

-

-

-

?

additional information

no substrates are amikacin

637299

Escherichia coli

?

-

-

-

?

additional information

no substrates are amikacin

637299

Pseudomonas aeruginosa

?

-

-

-

?

additional information

no substrates are amikacin

637299

Pseudomonas aeruginosa Travers PST

?

-

-

-

?

additional information

no substrates are amikacin

637299

Escherichia coli R135/C600

?

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

756710

Ung

Crystal structure of the amin ...

Mycobacteroides abscessus, Mycobacteroides abscessus DSM 44196

FEBS J.

286

4342-4355

2019

-

-

1

1

-

-

-

1

-

-

-

-

-

2

-

-

-

-

-

-

-

-

2

-

2

-

-

-

1

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

757396

Kumar

Encoding of promiscuity in an ...

Pseudomonas aeruginosa

J. Med. Chem.

61

10218-10227

2018

-

-

-

1

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

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3

-

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-

1

-

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-

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-

-

-

-

-

-

-

-

758305

Kumar

Thermodynamics of an aminogly ...

Enterobacter cloacae

Proteins

85

1258-1265

2017

-

-

1

-

-

-

-

5

-

-

-

-

-

1

-

-

-

-

-

-

-

-

6

1

2

-

-

-

5

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

5

-

-

-

-

-

-

-

-

-

-

-

-

6

1

-

-

-

5

-

-

-

-

-

-

-

-

5

5

757385

Garzan

Sulfonamide-based inhibitors ...

Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv

J. Med. Chem.

59

10619-10628

2016

-

-

-

1

1

-

2

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

1

-

-

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-

-

-

-

-

-

-

2

-

-

-

-

1

1

-

2

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

735448

Galimand

AAC(3)-XI, a new aminoglycosid ...

Corynebacterium striatum, Corynebacterium striatum BM4687

Antimicrob. Agents Chemother.

59

5647-5653

2015

-

-

1

-

-

-

1

7

-

-

-

6

-

17

-

-

1

-

-

2

-

-

18

-

3

1

-

-

6

1

-

-

1

-

-

-

-

-

1

1

-

-

-

-

1

-

7

-

-

-

6

-

-

-

1

-

2

-

-

18

-

1

-

-

6

1

-

-

-

-

2

2

-

6

6

736260

Atasoy

Modifying enzymes related amin ...

Acinetobacter baumannii

Int. J. Clin. Exp. Med.

8

2874-2880

2015

-

-

1

-

-

-

1

-

-

-

-

1

-

1

-

-

-

-

-

1

-

-

1

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

1

-

-

-

-

-

1

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

735678

Norris

Protein dynamics are influence ...

Pseudomonas aeruginosa

Biochemistry

53

30-38

2014

-

-

-

-

-

-

-

4

-

-

-

-

-

1

-

-

-

-

-

-

-

-

8

-

1

-

-

-

7

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

8

-

-

-

-

7

-

-

-

-

-

-

-

-

4

4

703205

Green

Exploring the substrate promis ...

Escherichia coli

ChemBioChem

11

119-126

2010

-

1

1

-

-

-

-

20

-

-

-

-

-

1

-

-

-

-

-

-

-

-

34

-

4

-

-

-

20

1

-

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

20

-

-

-

-

-

-

-

-

-

-

-

-

34

-

-

-

-

20

1

-

-

-

-

-

-

-

-

-

718870

Norris

Thermodynamics and kinetics of ...

Pseudomonas aeruginosa

Biochemistry

49

4027-4035

2010

-

-

1

-

-

-

5

8

-

-

-

-

-

1

-

-

-

-

-

-

-

-

9

-

1

-

-

-

8

-

-

-

-

2

-

-

-

-

1

-

-

-

-

-

5

2

8

-

-

-

-

-

-

-

-

-

-

-

-

9

-

-

-

-

8

-

-

-

-

-

-

-

-

8

8

718871

Norris

Interactions of coenzyme A wit ...

Pseudomonas aeruginosa

Biochemistry

49

4036-4042

2010

-

-

-

-

-

-

-

4

-

-

-

-

-

1

-

-

-

-

-

-

-

-

5

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

5

-

-

-

-

4

-

-

-

-

-

-

-

-

4

4

703467

Cabrera

Characterization of the enzyme ...

Salmonella enterica

Enferm. Infecc. Microbiol. Clin.

27

453-456

2009

-

-

1

-

-

-

-

-

-

-

-

1

-

7

-

-

-

-

-

-

-

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1

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2

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-

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-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

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-

-

-

-

-

-

-

-

-

-

-

-

671371

Elbourne

Gene cassette encoding a 3-N-a ...

Saccharophagus degradans

Antimicrob. Agents Chemother.

50

2270-2271

2006

-

-

-

-

-

-

-

-

1

-

-

-

-

3

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

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-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

657558

Levings

New integron-associated gene c ...

Salmonella enterica subsp. enterica serovar Kentucky

Antimicrob. Agents Chemother.

49

1238-1241

2005

-

-

-

-

-

-

-

-

-

-

-

-

-

3

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

672007

Magalhaes

The kinetic mechanism of AAC3- ...

Escherichia coli, Escherichia coli BL21(DE3) pLysS

Biochemistry

44

16275-16283

2005

-

-

1

-

-

-

3

15

-

-

4

-

-

7

-

-

1

-

-

-

-

-

20

1

3

-

-

-

15

1

-

-

-

-

1

-

-

-

1

-

-

-

-

-

3

-

15

-

-

4

-

-

-

-

1

-

-

-

-

20

1

-

-

-

15

1

-

-

1

-

-

-

-

-

-

672534

Welch

Discovery of non-carbohydrate ...

Homo sapiens

Bioorg. Med. Chem.

13

6252-6263

2005

-

-

1

-

-

-

3

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

3

-

-

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-

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1

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-

-

-

-

-

-

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675934

Subba

The ribostamycin biosynthetic ...

Streptomyces ribosidificus

Mol. Cell

20

90-96

2005

-

-

1

-

-

-

-

-

-

-

1

-

-

4

-

-

1

-

-

-

-

-

1

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

1

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-

-

-

1

-

-

-

-

1

-

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-

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-

-

-

-

-

-

-

-

-

487553

Riccio

Novel 3-N-aminoglycoside acety ...

Pseudomonas aeruginosa

Antimicrob. Agents Chemother.

47

1746-1748

2003

-

-

1

-

-

-

-

-

1

-

-

-

-

7

-

-

-

-

-

-

-

-

10

-

1

-

-

-

-

-

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-

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-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

10

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

487552

Tsuchizaki

-

Rapid characterization by colo ...

Streptomyces griseus

Actinomycetologica

15

23-29

2001

-

-

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

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-

-

-

-

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-

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-

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-

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1

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-

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-

-

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-

-

-

-

-

487551

Ishikawa

Identification and characteriz ...

Streptomyces griseus

Antimicrob. Agents Chemother.

44

437-440

2000

-

1

1

-

-

-

-

-

2

-

-

-

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4

-

-

-

-

-

-

-

-

1

-

1

-

-

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-

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-

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-

1

1

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-

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-

-

2

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-

-

1

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-

-

-

-

-

-

-

-

487550

Wolf

Crystal structure of a GCN5-re ...

Serratia marcescens

Cell

94

439-449

1998

-

-

1

1

-

-

-

-

1

-

1

-

-

8

-

1

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

1

-

1

-

-

-

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

637305

Gomez-Lus

Stability of dactimicin to ami ...

Escherichia coli

Drugs Exp. Clin. Res.

15

129-132

1989

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

637304

Bongaerts

Effect of aminoglycoside conce ...

Escherichia coli, Escherichia coli JR88

Antimicrob. Agents Chemother.

32

740-746

1988

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

1

-

-

-

-

-

10

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

10

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

637300

Angelatou

Purification and properties of ...

Pseudomonas aeruginosa

J. Antibiot.

35

235-244

1982

-

-

1

-

-

2

3

1

-

3

1

-

-

3

-

-

1

-

-

-

1

2

11

-

-

1

-

1

-

1

-

-

-

-

-

-

-

-

1

-

-

-

2

-

3

-

1

-

3

1

-

-

-

-

1

-

-

1

2

11

-

1

-

1

-

1

-

-

-

-

-

-

-

-

-

661049

Coombe

Purification and properties of ...

Pseudomonas aeruginosa

Biochemistry

21

871-875

1982

-

-

-

-

-

1

2

1

-

-

1

-

-

4

-

-

1

-

-

-

2

-

1

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

2

-

1

-

-

1

-

-

-

-

1

-

-

2

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

487549

Davies

Enzymatic modification of amin ...

Arizona sp., Escherichia coli, Klebsiella pneumoniae

Antimicrob. Agents Chemother.

14

69-72

1978

-

3

2

-

-

-

-

-

3

-

-

-

-

3

-

-

-

-

-

-

-

-

3

-

2

-

-

-

-

-

-

-

-

-

-

-

-

3

2

-

-

-

-

-

-

-

-

3

-

-

-

-

-

-

-

-

-

-

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

637301

Williams

Kinetic mechanisms of gentamic ...

Escherichia coli, Escherichia coli C600

J. Biol. Chem.

253

5902-5907

1978

-

-

-

-

-

-

9

5

-

-

-

-

-

7

-

-

-

-

-

-

-

-

6

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

9

-

5

-

-

-

-

-

-

-

-

-

-

-

-

6

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

637299

Biddlecome

Enzymatic modification of amin ...

Escherichia coli, Pseudomonas aeruginosa, Pseudomonas aeruginosa Travers PST, Escherichia coli R135/C600

Antimicrob. Agents Chemother.

9

951-955

1976

-

-

-

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

22

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

22

-

2

-

-

-

-

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-

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