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Literature summary for 2.3.1.8 extracted from
- Purification and characterization of two extremely thermostable enzymes, phosphate acetyltransferase and acetate kinase, from the hyperthermophilic eubacterium Thermotoga maritima (1999), J. Bacteriol., 181, 1861-1867.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0231 | - |
acetyl-CoA | - |
Thermotoga maritima |  |
| 0.024 | - |
acetyl phosphate | - |
Thermotoga maritima | |
| 0.03 | - |
CoA | - |
Thermotoga maritima | |
| 0.111 | - |
phosphate | - |
Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
alpha4, homotetramer, 4 * 34000, SDS-PAGE | Thermotoga maritima |
| 170000 | - |
gel filtration | Thermotoga maritima |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + phosphate | Thermotoga maritima | - |
CoA + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
hyperthermophilic eubacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| apparent homogeneity | Thermotoga maritima |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 225 | - |
- |
Thermotoga maritima |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, no loss of activity for several weeks | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + phosphate | - |
Thermotoga maritima | CoA + acetyl phosphate | - |
r | |
| butyryl-CoA + phosphate | - |
Thermotoga maritima | CoA + butyryl phosphate | - |
? | |
| propionyl-CoA + phosphate | - |
Thermotoga maritima | CoA + propionyl phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha4, homotetramer, 4 * 34000, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
stable for 120 min | Thermotoga maritima |
| 90 | - |
60% loss of activity after 120 min | Thermotoga maritima |
| 100 | - |
60% loss of activity after 120 min | Thermotoga maritima |
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