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Literature summary for 2.3.1.8 extracted from

  • Kreuzberg, K.; Umlauf, H.; Blaschkowski, H.P.
    Purification and properties of phosphotransacetylase from the eucaryotic green alga Chlorogonium elongatum (1985), Biochim. Biophys. Acta, 842, 22-29.
No PubMed abstract available
Search for more literature for 2.3.1.8

General Stability

General Stability Organism
stabilized by addition of 200 mM ammonium sulfate and 2-5 mM mercaptans to the extraction buffer Chlorogonium elongatum

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA competitive Chlorogonium elongatum
ADP no effect Chlorogonium elongatum
ATP no effect Chlorogonium elongatum
iodoacetamide 70% inactivation after 1 min at 5 mM Chlorogonium elongatum
iodoacetic acid 76% inactivation after 4 min at 5 mM Chlorogonium elongatum
phosphate competitive Chlorogonium elongatum
S-dimethylarsino-CoA
-
 Chlorogonium elongatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
 acetyl-CoA
-
 Chlorogonium elongatum
0.09
-
 CoA arsenolysis Chlorogonium elongatum
0.093
-
 CoA
-
 Chlorogonium elongatum
0.311
-
 acetyl phosphate
-
 Chlorogonium elongatum
0.66
-
 acetyl phosphate
-
 Chlorogonium elongatum
5.44
-
 phosphate
-
 Chlorogonium elongatum

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
 Chlorogonium elongatum
NH4+
-
 Chlorogonium elongatum
Tris maximum activity with Tris buffer, with HEPES and MES catalysis is lowered by ca. 15% Chlorogonium elongatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 6 * 44000, SDS-PAGE Chlorogonium elongatum
280000
-
 gel filtration, SDS-PAGE Chlorogonium elongatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + phosphate Chlorogonium elongatum
-
 CoA + acetyl phosphate
-
 r
additional information Chlorogonium elongatum physiological function in anaerobic metabolism of eukaryotic green algae rather than in aerobic acetate activation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Chlorogonium elongatum
-
 green alga
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chlorogonium elongatum

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + phosphate = CoA + acetyl phosphate rapid equilibrium random bi-bi reaction mechanism Chlorogonium elongatum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 assay methods Chlorogonium elongatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + phosphate
-
 Chlorogonium elongatum CoA + acetyl phosphate
-
 r
additional information physiological function in anaerobic metabolism of eukaryotic green algae rather than in aerobic acetate activation Chlorogonium elongatum ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 44000, SDS-PAGE Chlorogonium elongatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 both directions Chlorogonium elongatum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 t1/2 18 min Chlorogonium elongatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 maximum activity with Tris buffer, with HEPES and MES catalysis is lowered by ca. 15% Chlorogonium elongatum
7.6
-
 both directions Chlorogonium elongatum