Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methyl methanethiolsulfonate | activates the membrane-bound enzyme 1.5fold | Rattus norvegicus | |
taurodeoxycholate | 2 to 3fold increase of activity | Rattus norvegicus | |
Triton X-100 | 2 to 3fold increase of activity | Rattus norvegicus |
General Stability | Organism |
---|---|
solubilized enzyme, not stable | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | membrane-bound enzyme is insensitive, solubilized enzyme is inactivated | Rattus norvegicus | |
alpha-D-glucosamine 6-phosphate | weak | Rattus norvegicus | |
alpha-methyl-D-glucose | weak | Rattus norvegicus | |
cellobiose | weak | Rattus norvegicus | |
chitin | - |
Rattus norvegicus | |
chitosan | - |
Rattus norvegicus | |
coenzyme A | noncompetitive | Rattus norvegicus | |
dithiothreitol | solubilized protein | Rattus norvegicus | |
maltose | weak | Rattus norvegicus | |
additional information | iodoacetamide and N-ethylmaleimide are not inhibitory | Rattus norvegicus | |
N-acetyl-alpha-D-glucosamine | - |
Rattus norvegicus | |
N-acetyl-beta-D-glucosamine | noncompetitive against acetyl-CoA | Rattus norvegicus | |
N-laurylsarcosine | 1% | Rattus norvegicus | |
p-chloromercuribenzoate | - |
Rattus norvegicus | |
sucrose | weak | Rattus norvegicus | |
Zwittergent | 1% | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | 0.3 | alpha-D-glucosamine | - |
Rattus norvegicus | |
0.55 | - |
acetyl-CoA | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + heparan sulfate alpha-D-glucosaminide | Rattus norvegicus | - |
CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + heparan sulfate alpha-D-glucosaminide = CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide | di-iso ping-pong mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-70°C, membrane-bound enzyme, 12 months stable | Rattus norvegicus |
4°C, solubilized enzyme, 25% loss of activity after 7 days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + alpha-D-glucosamine | - |
Rattus norvegicus | CoA + N-acetyl-alpha-D-glucosamine | - |
? | |
acetyl-CoA + heparan sulfate alpha-D-glucosaminide | - |
Rattus norvegicus | CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide | - |
? | |
acetyl-CoA + heparan sulfate alpha-D-glucosaminide | brings about the acetylation of glucosamine groups of heparan sulfate and heparin from which the sulfate has been removed | Rattus norvegicus | CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
membrane-bound form | Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | - |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
5 min, solubilized enzyme, 50% loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
above, broad maximum | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 7 | coenzyme A | - |
Rattus norvegicus | |
15 | 17 | N-acetyl-alpha-D-glucosamine | - |
Rattus norvegicus |