Cloned (Comment) | Organism |
---|---|
FLAG-tagged enzyme expression in COS-7 cells, the cells expressing DGAT1 show ARAT and WAX synthase, EC 2.3.1.75, activities | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | cells and tissues from DGAT1-deficient mice exhibit reduced ARAT activity, and the mice have increased levels of unesterified retinol in their livers on a high-retinol diet | Mus musculus |
additional information | DGAT1 deficiency also perturbs retinol metabolism in the livers of Dgat-/- mice, hepatic levels of unesterified retinol are increased in Dgat-/- mice challenged with high-retinol diets | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mus musculus | 16020 | - |
membrane | the enzyme is is very hydrophobic and has multiple predicted membrane-spanning domains | Mus musculus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans retinol + palmitoyl-CoA | Mus musculus | - |
all-trans-retinyl palmitate + CoA | - |
? | |
additional information | Mus musculus | ARAT plays a role in retinol metabolism in tissues with low levels of cytosolic retinol binding protein or when cellular retinol concentrations are relatively high, thereby protecting cells from toxicity resulting from excess unesterified retinol | ? | - |
? | |
additional information | Mus musculus | cells and tissues from DGAT1-deficient mice exhibit reduced ARAT activity, and the mice have increased levels of unesterified retinol in their livers on a high-retinol diet | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
additional information | cells and tissues from DGAT1-deficient mice exhibit reduced ARAT activity | Mus musculus | - |
additional information | DGAT1 exhibits ARAT activity in in vitro assays and accounts for a large portion of ARAT activity in the liver, testis, and kidney of mice | Mus musculus | - |
testis | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
measurements of retinol and retinyl esters in liver | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans retinol + palmitoyl-CoA | - |
Mus musculus | all-trans-retinyl palmitate + CoA | - |
? | |
additional information | ARAT plays a role in retinol metabolism in tissues with low levels of cytosolic retinol binding protein or when cellular retinol concentrations are relatively high, thereby protecting cells from toxicity resulting from excess unesterified retinol | Mus musculus | ? | - |
? | |
additional information | cells and tissues from DGAT1-deficient mice exhibit reduced ARAT activity, and the mice have increased levels of unesterified retinol in their livers on a high-retinol diet | Mus musculus | ? | - |
? | |
additional information | DGAT1 is catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the diacylglycerol transferase, EC 2.3.1.20, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview | Mus musculus | ? | - |
? | |
additional information | the triacylglycerol synthesis enzyme DGAT1, EC 2.3.1.20, also catalyzes the synthesis of diacylglycerols, waxes, and retinyl esters, overview | Mus musculus | ? | - |
? | |
palmitoyl-CoA + retinol | - |
Mus musculus | CoA + retinyl palmitate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acyl CoA:retinol acyltransferase | - |
Mus musculus |
acyl CoA:retinol O-acyltransferase | - |
Mus musculus |
ArAT | - |
Mus musculus |
More | cf. EC 2.3.1.20 | Mus musculus |