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Literature summary for 2.3.1.76 extracted from

  • Yen, C.L.; Monetti, M.; Burri, B.J.; Farese, R.V.
    The triacylglycerol synthesis enzyme DGAT1 also catalyzes the synthesis of diacylglycerols, waxes, and retinyl esters (2005), J. Lipid Res., 46, 1502-1511.
    View publication on PubMed
Search for more literature for 2.3.1.76

Cloned(Commentary)

Cloned (Comment) Organism
FLAG-tagged enzyme expression in COS-7 cells, the cells expressing DGAT1 show ARAT and WAX synthase, EC 2.3.1.75, activities Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information cells and tissues from DGAT1-deficient mice exhibit reduced ARAT activity, and the mice have increased levels of unesterified retinol in their livers on a high-retinol diet Mus musculus
additional information DGAT1 deficiency also perturbs retinol metabolism in the livers of Dgat-/- mice, hepatic levels of unesterified retinol are increased in Dgat-/- mice challenged with high-retinol diets Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mus musculus 16020
-
membrane the enzyme is is very hydrophobic and has multiple predicted membrane-spanning domains Mus musculus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
all-trans retinol + palmitoyl-CoA Mus musculus
-
 all-trans-retinyl palmitate + CoA
-
 ?
additional information Mus musculus ARAT plays a role in retinol metabolism in tissues with low levels of cytosolic retinol binding protein or when cellular retinol concentrations are relatively high, thereby protecting cells from toxicity resulting from excess unesterified retinol ?
-
 ?
additional information Mus musculus cells and tissues from DGAT1-deficient mice exhibit reduced ARAT activity, and the mice have increased levels of unesterified retinol in their livers on a high-retinol diet ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Mus musculus
-
liver
-
 Mus musculus
-
additional information cells and tissues from DGAT1-deficient mice exhibit reduced ARAT activity Mus musculus
-
additional information DGAT1 exhibits ARAT activity in in vitro assays and accounts for a large portion of ARAT activity in the liver, testis, and kidney of mice Mus musculus
-
testis
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 measurements of retinol and retinyl esters in liver Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans retinol + palmitoyl-CoA
-
 Mus musculus all-trans-retinyl palmitate + CoA
-
 ?
additional information ARAT plays a role in retinol metabolism in tissues with low levels of cytosolic retinol binding protein or when cellular retinol concentrations are relatively high, thereby protecting cells from toxicity resulting from excess unesterified retinol Mus musculus ?
-
 ?
additional information cells and tissues from DGAT1-deficient mice exhibit reduced ARAT activity, and the mice have increased levels of unesterified retinol in their livers on a high-retinol diet Mus musculus ?
-
 ?
additional information DGAT1 is catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the diacylglycerol transferase, EC 2.3.1.20, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview Mus musculus ?
-
 ?
additional information the triacylglycerol synthesis enzyme DGAT1, EC 2.3.1.20, also catalyzes the synthesis of diacylglycerols, waxes, and retinyl esters, overview Mus musculus ?
-
 ?
palmitoyl-CoA + retinol
-
 Mus musculus CoA + retinyl palmitate
-
 ?

Synonyms

Synonyms Comment Organism
acyl CoA:retinol acyltransferase
-
 Mus musculus
acyl CoA:retinol O-acyltransferase
-
 Mus musculus
ArAT
-
 Mus musculus
More cf. EC 2.3.1.20 Mus musculus