BRENDA - Enzyme Database show
show all sequences of 2.3.1.76

Assay of lecithin-retinol acyltransferase

MacDonald, P.N.; Ong, D.E.; Methods Enzymol. 189, 450-459 (1990)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
retinyl esterification: storage of vitamin A
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
cellular retinol-binding protein
specific inhibition of retinol fatty-acyltransferase by capturing substrate
Rattus norvegicus
additional information
not inhibited by PMSF
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
membrane fraction
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acyl-CoA + retinol
Rattus norvegicus
-
CoA + retinyl ester
-
Rattus norvegicus
?
acyl-CoA + retinol
Rattus norvegicus Sprague-Dawley
-
CoA + retinyl ester
-
Rattus norvegicus Sprague-Dawley
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
Sprague-Dawley
-
Purification (Commentary)
Commentary
Organism
partial purification by differential centrifugation
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
small intestine
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
activity determined from microsomes
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-70ºC, phosphate buffer, pH 7.2, DTT, 12 months
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acyl-CoA + retinol
-
487493
Rattus norvegicus
CoA + retinyl ester
-
487493
Rattus norvegicus
?
acyl-CoA + retinol
-
487493
Rattus norvegicus Sprague-Dawley
CoA + retinyl ester
-
487493
Rattus norvegicus Sprague-Dawley
?
palmitoyl-CoA + retinol
-
487493
Rattus norvegicus
CoA + retinylpalmitate
-
487493
Rattus norvegicus
?
palmitoyl-CoA + retinol
-
487493
Rattus norvegicus Sprague-Dawley
CoA + retinylpalmitate
-
487493
Rattus norvegicus Sprague-Dawley
?
Application (protein specific)
Application
Commentary
Organism
medicine
retinyl esterification: storage of vitamin A
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cellular retinol-binding protein
specific inhibition of retinol fatty-acyltransferase by capturing substrate
Rattus norvegicus
additional information
not inhibited by PMSF
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
membrane fraction
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acyl-CoA + retinol
Rattus norvegicus
-
CoA + retinyl ester
-
Rattus norvegicus
?
acyl-CoA + retinol
Rattus norvegicus Sprague-Dawley
-
CoA + retinyl ester
-
Rattus norvegicus Sprague-Dawley
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial purification by differential centrifugation
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
small intestine
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
activity determined from microsomes
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-70ºC, phosphate buffer, pH 7.2, DTT, 12 months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acyl-CoA + retinol
-
487493
Rattus norvegicus
CoA + retinyl ester
-
487493
Rattus norvegicus
?
acyl-CoA + retinol
-
487493
Rattus norvegicus Sprague-Dawley
CoA + retinyl ester
-
487493
Rattus norvegicus Sprague-Dawley
?
palmitoyl-CoA + retinol
-
487493
Rattus norvegicus
CoA + retinylpalmitate
-
487493
Rattus norvegicus
?
palmitoyl-CoA + retinol
-
487493
Rattus norvegicus Sprague-Dawley
CoA + retinylpalmitate
-
487493
Rattus norvegicus Sprague-Dawley
?
Other publictions for EC 2.3.1.76
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
704611
Shih
Retinol esterification by DGAT ...
Mus musculus
J. Biol. Chem.
284
4292-4299
2009
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1
-
-
-
-
-
-
-
-
-
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3
-
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1
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1
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1
-
-
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-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
672075
Muniz
11-cis-Acyl-CoA:retinol O-acyl ...
Gallus gallus
Biochemistry
45
12265-12273
2006
1
-
-
-
-
-
1
1
1
-
-
2
-
2
-
-
1
-
-
3
2
-
4
-
1
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-
-
1
-
-
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-
-
-
1
-
-
-
-
-
-
-
1
-
1
1
-
-
2
-
-
-
1
-
3
2
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
658662
Kaschula
Acyl CoA:retinol acyltransfera ...
Bos taurus, Homo sapiens
Exp. Eye Res.
82
111-121
2005
-
-
2
-
-
-
3
7
3
-
-
4
-
4
-
-
-
1
-
5
-
-
7
-
2
-
-
-
2
-
-
-
2
-
1
-
-
2
-
-
-
-
1
3
2
7
3
-
-
4
-
-
-
-
-
5
-
-
7
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
659670
Yen
A human skin multifunctional O ...
Homo sapiens
J. Lipid Res.
46
2388-2397
2005
-
-
1
-
-
-
-
-
1
1
-
2
-
2
-
-
-
-
-
6
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
6
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
672340
Orland
Acyl coenzyme A dependent reti ...
Homo sapiens
Biochim. Biophys. Acta
1737
76-82
2005
-
-
1
-
-
-
1
2
1
-
-
1
-
1
-
-
-
-
-
1
2
-
3
-
1
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
1
1
1
2
1
-
-
1
-
-
-
-
-
1
2
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
675260
Yen
The triacylglycerol synthesis ...
Mus musculus
J. Lipid Res.
46
1502-1511
2005
-
-
1
-
1
-
-
-
1
-
-
3
-
2
-
-
-
-
-
4
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
4
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487496
Jurukovski
Reduced lecithin:retinol acyl ...
Homo sapiens
Biochim. Biophys. Acta
1436
479-490
1999
1
1
-
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
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-
1
-
-
1
-
-
-
1
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487497
Ndayibagira
-
Esterification and hydrolysis ...
Salvelinus fontinalis
Comp. Biochem. Physiol. C
122C
317-325
1999
-
1
-
-
-
-
1
1
1
-
-
1
-
1
-
-
1
-
-
1
1
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2
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1
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1
1
2
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1
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1
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1
1
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1
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1
-
1
1
-
2
-
1
-
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-
1
1
2
-
-
-
-
-
-
-
487494
Nilsson
2,3,7,8-Tetrachlorodibenzo-p-d ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Environ. Toxicol. Pharmacol.
2
17-23
1996
-
-
-
-
-
-
1
-
1
-
-
2
-
162
-
-
1
-
-
2
1
-
4
-
-
-
-
-
-
-
1
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-
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-
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-
-
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1
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1
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2
-
-
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1
-
2
1
-
4
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
487495
Ross
Radiation inactivation analysi ...
Rattus norvegicus
J. Lipid Res.
34
1201-1207
1993
-
1
-
-
-
-
-
-
1
-
-
1
-
2
-
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1
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2
1
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2
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1
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1
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1
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1
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1
-
2
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
487489
Ball
Acyl coenzyme A-dependent reti ...
Rattus norvegicus
Methods Enzymol.
189
446-449
1990
-
-
-
-
-
-
-
-
1
-
-
1
-
1
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1
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-
1
1
1
2
-
1
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1
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1
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1
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1
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1
1
1
2
-
1
-
-
-
-
-
1
-
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-
-
-
-
487490
Ross
Measurement of acyl coenzyme A ...
Rattus norvegicus
Methods Enzymol.
189
442-445
1990
-
-
-
-
-
-
1
-
1
-
-
1
-
1
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1
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1
1
1
2
-
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1
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1
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1
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1
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1
-
1
1
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
487491
Saari
Acyl-CoA:retinol acyltransfera ...
Bos taurus
Methods Enzymol.
190
156-163
1990
-
1
-
-
-
-
-
-
1
-
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1
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2
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1
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2
1
1
3
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1
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1
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1
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1
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1
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2
1
1
3
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
487493
MacDonald
Assay of lecithin-retinol acyl ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Methods Enzymol.
189
450-459
1990
-
1
-
-
-
-
2
-
1
-
-
2
-
162
-
-
1
-
-
1
1
1
4
-
-
-
-
-
-
-
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-
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1
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-
-
-
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2
-
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1
-
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2
-
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-
1
-
1
1
1
4
-
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-
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-
-
-
-
-
-
-
-
-
-
487492
Chaudhary
Some properties and subcellula ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Biochim. Biophys. Acta
917
24-32
1987
2
1
-
-
-
-
5
-
1
-
-
2
-
162
-
-
1
-
-
1
1
-
8
-
-
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
5
-
-
1
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2
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-
-
1
-
1
1
-
8
-
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-
1
-
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-
1
-
-
-
-
-
-
-
487488
Ross
Retinol esterification by rat ...
Felis silvestris, Rattus norvegicus, Rattus norvegicus Sprague-Dawley
J. Biol. Chem.
257
2453-2459
1982
2
-
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-
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5
-
2
-
-
2
-
165
-
-
2
-
-
2
2
1
13
-
1
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1
1
2
-
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-
2
-
-
-
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-
-
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5
-
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2
-
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2
-
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-
2
-
2
2
1
13
-
1
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1
1
2
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