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Literature summary for 2.3.1.75 extracted from

  • Shi, S.; Valle-Rodriguez, J.O.; Siewers, V.; Nielsen, J.
    Engineering of chromosomal wax ester synthase integrated Saccharomyces cerevisiae mutants for improved biosynthesis of fatty acid ethyl esters (2014), Biotechnol. Bioeng., 111, 1740-1747.
    View publication on PubMed

Application

Application Comment Organism
synthesis synthesis of fatty acid ethyl esters in Saccharomyces cerevisiae by gene integration into chromosomal delta sequences using repetitive transformation, which results in 1-6 copies of the integration construct per genome. The corresponding fatty acid ethyl ester production increases up to 34 mg/L, an about sixfold increase compared to the equivalent plasmid-based producer. The cassette in the yeast genome is stably maintained in nonselective medium after deletion of RAD52. Additional overexprerssion of genes encoding endogenous acyl-CoA binding protein and a bacterial NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase (gapN) leads to another 40% increase in fatty acid ethyl ester production Marinobacter nauticus

Cloned(Commentary)

Cloned (Comment) Organism
delta integration in Saccharomyces cerevisiae, resulting in 1–6 copies of the integration construct per genome Marinobacter nauticus

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Marinobacter nauticus A3RE51
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