Application | Comment | Organism |
---|---|---|
synthesis | synthesis of fatty acid ethyl esters in Saccharomyces cerevisiae by gene integration into chromosomal delta sequences using repetitive transformation, which results in 1-6 copies of the integration construct per genome. The corresponding fatty acid ethyl ester production increases up to 34 mg/L, an about sixfold increase compared to the equivalent plasmid-based producer. The cassette in the yeast genome is stably maintained in nonselective medium after deletion of RAD52. Additional overexprerssion of genes encoding endogenous acyl-CoA binding protein and a bacterial NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase (gapN) leads to another 40% increase in fatty acid ethyl ester production | Marinobacter nauticus |
Cloned (Comment) | Organism |
---|---|
delta integration in Saccharomyces cerevisiae, resulting in 16 copies of the integration construct per genome | Marinobacter nauticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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Organism | UniProt | Comment | Textmining |
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Marinobacter nauticus | A3RE51 | - |
- |