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Literature summary for 2.3.1.75 extracted from

  • Barney, B.; Mann, R.; Ohlert, J.
    Identification of a residue affecting fatty alcohol selectivity in wax ester synthase (2013), Appl. Environ. Microbiol., 79, 396-399.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Acinetobacter baylyi
-
Marinobacter nauticus

Protein Variants

Protein Variants Comment Organism
A360F mutation slightly improves the rate of reaction found for smaller fatty alcohols Marinobacter nauticus
A360I mutation improves the rate of reaction found for smaller fatty alcohols Marinobacter nauticus
A360V mutation improves the rate of reaction found for smaller fatty alcohols Marinobacter nauticus
G355I mutation improves the rate of reaction Acinetobacter baylyi

Organism

Organism UniProt Comment Textmining
Acinetobacter baylyi Q8GGG1
-
-
Marinobacter nauticus A1TX06
-
-
Marinobacter nauticus DSM 11845 A1TX06
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
substrate hexadecan-1-ol, mutant A360I, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
1.64
-
substrate hexadecan-1-ol, mutant A360V, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
1.85
-
substrate hexadecan-1-ol, mutant A360F, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
2.7
-
substrate hexadecan-1-ol, wild-type, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
3.6
-
substrate nonan-1-ol, wild-type, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
7.8
-
substrate nonan-1-ol, mutant A360F, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
16.6
-
substrate nonan-1-ol, mutant A360V, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
19.6
-
substrate nonan-1-ol, mutant A360I, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
34.3
-
substrate dodecan-1-ol, mutant A360I, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
34.6
-
substrate dodecan-1-ol, mutant A360F, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
35.9
-
substrate dodecan-1-ol, mutant A360V, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
46.5
-
substrate dodecan-1-ol, wild-type, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
70
-
substrate hexadecan-1-ol, wild-type, pH not specified in the publication, temperature not specified in the publication Acinetobacter baylyi
80
-
substrate hexadecan-1-ol, mutant G355I, pH not specified in the publication, temperature not specified in the publication Acinetobacter baylyi
140
-
substrate nonan-1-ol, wild-type, pH not specified in the publication, temperature not specified in the publication Acinetobacter baylyi
360
-
substrate nonan-1-ol, mutant G355I, pH not specified in the publication, temperature not specified in the publication Acinetobacter baylyi
960
-
substrate dodecan-1-ol, wild-type, pH not specified in the publication, temperature not specified in the publication Acinetobacter baylyi
1700
-
substrate dodecan-1-ol, mutant G355I, pH not specified in the publication, temperature not specified in the publication Acinetobacter baylyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dodecan-1-ol + palmitoyl-CoA
-
Acinetobacter baylyi CoA + dodecanyl palmitate
-
?
dodecan-1-ol + palmitoyl-CoA
-
Marinobacter nauticus CoA + dodecanyl palmitate
-
?
dodecan-1-ol + palmitoyl-CoA
-
Marinobacter nauticus DSM 11845 CoA + dodecanyl palmitate
-
?
hexadecan-1-ol + palmitoyl-CoA
-
Acinetobacter baylyi CoA + hexadecyl palmitate
-
?
hexadecan-1-ol + palmitoyl-CoA
-
Marinobacter nauticus CoA + hexadecyl palmitate
-
?
hexadecan-1-ol + palmitoyl-CoA
-
Marinobacter nauticus DSM 11845 CoA + hexadecyl palmitate
-
?
nonan-1-ol + palmitoyl-CoA
-
Acinetobacter baylyi CoA + nonyl palmitate
-
?
nonan-1-ol + palmitoyl-CoA
-
Marinobacter nauticus CoA + nonyl palmitate
-
?
nonan-1-ol + palmitoyl-CoA
-
Marinobacter nauticus DSM 11845 CoA + nonyl palmitate
-
?