Cloned (Comment) | Organism |
---|---|
expression as soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Acinetobacter sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the knockout mutant Acinetobacter sp. strain ADP1acr1omegaKm is unable to produce fatty alcohols | Acinetobacter sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Acinetobacter baylyi ADP1 | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Acinetobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hexadecanethiol + palmitoyl-CoA | Acinetobacter sp. | mutant strain ADP1acr1omegaKm | palmitic acid hexadecyl thio ester + CoA | - |
? | |
1-hexadecanol + palmitoyl-CoA | Acinetobacter sp. | mutant strain ADP1acr1omegaKm | palmitic acid hexadecyl ester + CoA | - |
? | |
1-S-monopalmitoyloctanedithiol + palmitoyl-CoA | Acinetobacter sp. | mutant strain ADP1acr1omegaKm | 1,8-S-dipalmitoyloctanedithiol + CoA | - |
? | |
2 1,8-octanedithiol + 3 palmitoyl-CoA | Acinetobacter sp. | mutant strain ADP1acr1omegaKm | 1-S-monopalmitoyloctanedithiol + 1,8-S-dipalmitoyloctanedithiol + 3 CoA | 1-S-monopalmitoyloctanedithiol is the main product | ? | |
additional information | Acinetobacter sp. | the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols | ? | - |
? | |
additional information | Acinetobacter sp. ADP1 | the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baylyi ADP1 | Q8GGG1 | bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, EC 2.3.1.75 and EC 2.3.1.20 resp., expression in Escherichia coli with His-tag | - |
Acinetobacter sp. | - |
formerly Acinetobacter calcoaceticus ADP1 | - |
Acinetobacter sp. ADP1 | - |
formerly Acinetobacter calcoaceticus ADP1 | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble N-terminally His-tagged enzyme 19.2fold from Escherichia coli strain BL21(DE3) by cation exchange and nickel affinity chromatography | Acinetobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.165 | - |
purified recombinant His-tagged enzyme | Acinetobacter sp. |
0.1652 | - |
35°C, pH 7.4 | Acinetobacter baylyi ADP1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,8-octanedithiol + palmitoyl-CoA | 59.3% the rate of hexadecanol, plus minor amounts of 1,8-dipalmitoyloctanedithiol | Acinetobacter baylyi ADP1 | 1-S-monopalmitoyloctanedithiol + CoA | - |
? | |
1-hexadecanethiol + palmitoyl-CoA | 10.4% the rate of hexanol | Acinetobacter baylyi ADP1 | palmitic acid hexadecane thio ester + CoA | - |
? | |
1-hexadecanethiol + palmitoyl-CoA | - |
Acinetobacter sp. | palmitic acid hexadecyl thio ester + CoA | - |
? | |
1-hexadecanethiol + palmitoyl-CoA | mutant strain ADP1acr1omegaKm | Acinetobacter sp. | palmitic acid hexadecyl thio ester + CoA | - |
? | |
1-hexadecanol + palmitoyl-CoA | best substrate | Acinetobacter sp. | palmitic acid hexadecyl ester + CoA | - |
? | |
1-hexadecanol + palmitoyl-CoA | mutant strain ADP1acr1omegaKm | Acinetobacter sp. | palmitic acid hexadecyl ester + CoA | - |
? | |
1-hexanol + palmitoyl-CoA | - |
Acinetobacter baylyi ADP1 | hexyl palmitate + CoA | - |
? | |
1-S-monopalmitoyloctanedithiol + palmitoyl-CoA | 13.4% the rate of hexanol | Acinetobacter baylyi ADP1 | 1,8-dipalmitoyloctanedithiol + CoA | - |
? | |
1-S-monopalmitoyloctanedithiol + palmitoyl-CoA | - |
Acinetobacter sp. | 1,8-S-dipalmitoyloctanedithiol + CoA | - |
? | |
1-S-monopalmitoyloctanedithiol + palmitoyl-CoA | mutant strain ADP1acr1omegaKm | Acinetobacter sp. | 1,8-S-dipalmitoyloctanedithiol + CoA | - |
? | |
2 1,8-octanedithiol + 3 palmitoyl-CoA | - |
Acinetobacter sp. | 1-S-monopalmitoyloctanedithiol + 1,8-S-dipalmitoyloctanedithiol + 3 CoA | 1-S-monopalmitoyloctanedithiol is the main product | ? | |
2 1,8-octanedithiol + 3 palmitoyl-CoA | mutant strain ADP1acr1omegaKm | Acinetobacter sp. | 1-S-monopalmitoyloctanedithiol + 1,8-S-dipalmitoyloctanedithiol + 3 CoA | 1-S-monopalmitoyloctanedithiol is the main product | ? | |
2 1,8-octanedithiol + 3 palmitoyl-CoA | - |
Acinetobacter sp. ADP1 | 1-S-monopalmitoyloctanedithiol + 1,8-S-dipalmitoyloctanedithiol + 3 CoA | 1-S-monopalmitoyloctanedithiol is the main product | ? | |
additional information | the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols | Acinetobacter sp. | ? | - |
? | |
additional information | the bifunctional enzyme catalyzes the reactions of the diacylglycerol transferase, EC 2.3.1.20, and of the wax synthase, EC 2.3.1.75, substrate specificity of the wax ester synthase activity with alkanethiols as alternative acyl acceptors | Acinetobacter sp. | ? | - |
? | |
additional information | the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols | Acinetobacter sp. ADP1 | ? | - |
? | |
additional information | the bifunctional enzyme catalyzes the reactions of the diacylglycerol transferase, EC 2.3.1.20, and of the wax synthase, EC 2.3.1.75, substrate specificity of the wax ester synthase activity with alkanethiols as alternative acyl acceptors | Acinetobacter sp. ADP1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 2.3.1.20 | Acinetobacter sp. |
unspecific bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase | - |
Acinetobacter sp. |
WS/DGAT | - |
Acinetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Acinetobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Acinetobacter sp. |