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Literature summary for 2.3.1.74 extracted from

  • Waki, T.; Mameda, R.; Nakano, T.; Yamada, S.; Terashita, M.; Ito, K.; Tenma, N.; Li, Y.; Fujino, N.; Uno, K.; Yamashita, S.; Aoki, Y.; Denessiouk, K.; Kawai, Y.; Sugawara, S.; Saito, K.; Yonekura-Sakakibara, K.; Morita, Y.; Hoshino, A.; Takahashi, S.; Nakayama, T.
    A conserved strategy of chalcone isomerase-like protein to rectify promiscuous chalcone synthase specificity (2020), Nat. Commun., 11, 870 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information type IV chalcone isomerase-like protein (CHIL) binds to the enzyme to serve as a rectifier - rather than an activator - of promiscuous enzyme; it enhances enzyme-catalyzed THC production and diminishes CTAL formation without enhancing total polyketide production, thereby facilitating the efficient influx of substrates from the general phenylpropanoid pathway to the flavonoid pathway Glycine max
additional information type IV chalcone isomerase-like protein (CHIL) binds to the enzyme to serve as a rectifier - rather than an activator - of promiscuous enzyme; it enhances enzyme-catalyzed THC production and diminishes CTAL formation without enhancing total polyketide production, thereby facilitating the efficient influx of substrates from the general phenylpropanoid pathway to the flavonoid pathway Antirrhinum majus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Glycine max
expressed in Escherichia coli BL21 cells Antirrhinum majus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00473
-
4-coumaroyl-CoA in the presence of 0.001 mM chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus
0.0497
-
4-coumaroyl-CoA in the absence of chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 malonyl-CoA + 4-coumaroyl-CoA Glycine max
-
4 CoA + naringenin chalcone + 3 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA Antirrhinum majus
-
4 CoA + naringenin chalcone + 3 CO2
-
?

Organism

Organism UniProt Comment Textmining
Antirrhinum majus
-
-
-
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Glycine max
-
Antirrhinum majus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 malonyl-CoA + 4-coumaroyl-CoA
-
Glycine max 4 CoA + naringenin chalcone + 3 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA
-
Antirrhinum majus 4 CoA + naringenin chalcone + 3 CO2
-
?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Glycine max
? x * 45000, SDS-PAGE Antirrhinum majus

Synonyms

Synonyms Comment Organism
CHS
-
Antirrhinum majus
chs-1 isoform Glycine max
CHS-7 isoform Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.087
-
4-coumaroyl-CoA in the presence of 0.001 mM chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus
0.14
-
malonyl-CoA in the presence of 0.001 mM chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus
0.59
-
malonyl-CoA in the absence of chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus
1.27
-
4-coumaroyl-CoA in the absence of chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
18.5
-
4-coumaroyl-CoA in the presence of 0.001 mM chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus
25.7
-
4-coumaroyl-CoA in the absence of chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus