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Literature summary for 2.3.1.74 extracted from

  • Hatayama, M.; Unno, H.; Kusunoki, M.; Takahashi, S.; Nishino, T.; Nakayama, T.
    Production of tetraketide lactones by mutated Antirrhinum majus chalcone synthases (AmCHS1) (2010), J. Biosci. Bioeng., 110, 158-164.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
T197A mutation caused no substantial enhancement of the p-coumaroyltriacetic acid lactone-producing activity Antirrhinum majus
V196M mutant produces non-chalcone product p-coumaroyltriacetic acid lactone from p-coumaroyl-CoA and malonyl-CoA. Mutation results in a loss of tetrahydroxychalcone-producing activity, as well as a 12.6fold enhancement of p-coumaroyltriacetic acid lactone-producing activity at pH 7.5. Wild-type enzyme shows low p-coumaroyltriacetic acid lactone-producing activity at pH 7.5, but an appreciable level at pH 10. Substitution V196M causes a shift toward neutrality of the optimum pH for p-coumaroyltriacetic acid lactone-producing activity Antirrhinum majus
V196M/T197A mutant produces significant amounts of non-chalcone product p-coumaroyltriacetic acid lactone from p-coumaroyl-CoA and malonyl-CoA, along with a small amount of 2',4,4',6'-tetrahydroxychalcone. Wild-type enzyme shows low p-coumaroyltriacetic acid lactone-producing activity at pH 7.5, but an appreciable level at pH 10. Substitution V196M/T197A causes a shift toward neutrality of the optimum pH for p-coumaroyltriacetic acid lactone-producing activity Antirrhinum majus

Organism

Organism UniProt Comment Textmining
Antirrhinum majus
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + 3 malonyl-CoA
-
 Antirrhinum majus 4-coumaroyltriacetic acid lactone + 4 CoA + 3 CO2 wild-type enzyme shows low 4-coumaroyltriacetic acid lactone-producing activity at pH 7.5, but an appreciable level at pH 10. Substitutions V196M, T197A, and V196M/T197A causes a shift toward neutrality of the optimum pH for 4-coumaroyltriacetic acid lactone-producing activity. Enhancement of the tetraketide producing activity upon V196M and V196M/T197A substitutions is most markedly observed when 4-coumaroyl-CoA is used as the starter substrate, and only slightly with benzoyl-, caffeoyl- and hexanoyl-CoA ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Antirrhinum majus