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Literature summary for 2.3.1.74 extracted from
- Enzymatic properties and mutational studies of chalcone synthase from Physcomitrella patens (2012), Int. J. Mol. Sci., 13, 9673-9691.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Physcomitrium patens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| molecular modeling of structure | Physcomitrium patens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C170R | more than 50% decrease in activity with hexanoyl-CoA, increase in thermal stability | Physcomitrium patens |
| C170S | more than 80% decrease in activity with hexanoyl-CoA, increase in thermal stability | Physcomitrium patens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
2 * 62000, SDS-PAGE | Physcomitrium patens |
| 120000 | - |
gel filtration | Physcomitrium patens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Physcomitrium patens | Q2VAZ3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3 malonyl-CoA + 4-coumaroyl-CoA | - |
Physcomitrium patens | 4 CoA + naringenin chalcone + 3 CO2 | - |
? |  |
| 3 malonyl-CoA + hexanoyl-CoA | 37% of the activity with 4-coumaroyl-CoA | Physcomitrium patens | 4 CoA + ? + 3 CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 62000, SDS-PAGE | Physcomitrium patens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CHS | - |
Physcomitrium patens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Physcomitrium patens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
stable up to | Physcomitrium patens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
substrate 4-coumaroyl-CoA | Physcomitrium patens |
| 8 | - |
substrate hexanoyl-CoA | Physcomitrium patens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Physcomitrium patens | calculated | - |
7.1 |
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Release 2023.1 (January 2023)
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