General Stability | Organism |
---|---|
5% activity after freezing for 1 h without presence of glycerol | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
4-coumaroyl-CoA | isozyme AI | Spinacia oleracea | |
0.001 | - |
4-coumaroyl-CoA | isozyme AII | Spinacia oleracea | |
0.0019 | - |
malonyl-CoA | isozyme AII | Spinacia oleracea | |
0.002 | - |
malonyl-CoA | isozyme AI | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | no activity in chloroplasts | Spinacia oleracea | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
isozyme AI, gel filtration, differences in MW may be due to different spatial conformations of AI and AII | Spinacia oleracea |
62000 | - |
isozyme AII, gel filtration, differences in MW may be due to different spatial conformations of AI and AII | Spinacia oleracea |
78000 | - |
isozyme AII, non-denaturing gel electrophoresis, differences in MW may be due to different spatial conformations of AI and AII | Spinacia oleracea |
88000 | - |
isozyme AI, non-denaturing gel electrophoresis, differences in MW may be due to different spatial conformations of AI and AII | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
spinach | - |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Storage Stability | Organism |
---|---|
-20°C, 0.4 M phosphate buffer, pH 6.5, 20% v/v glycerol, 4 weeks | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + 4-coumaroyl-CoA | - |
Spinacia oleracea | naringenin chalcone + CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 78000 + 1 * 88000, non-denaturating PAGE, 1 * 48000 + 1 * 62000, gel filtration | Spinacia oleracea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
half-life 4-5 d, partially purified enzyme | Spinacia oleracea |