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Literature summary for 2.3.1.7 extracted from

  • Cordente, A.G.; Lopez-Vinas, E.; Vazquez, M.I.; Swiegers, J.H.; Pretorius, I.S.; Gomez-Puertas, P.; Hegardt, F.G.; Asins, G.; Serra, D.
    Redesign of carnitine acetyltransferase specificity by protein engineering (2004), J. Biol. Chem., 279, 33899-33908.
    View publication on PubMed
Search for more literature for 2.3.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae and Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
E347A complete loss of activity Rattus norvegicus
H343A complete loss of activity Rattus norvegicus
M564A lowered activity with acetyl-CoA, increased activity with longer chain acyl-CoAs Rattus norvegicus
M564G lowered activity with acetyl-CoA, increased activity with longer chain acyl-CoAs, 1250-fold increase in activity with myristoyl-CoA Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 octanoyl-CoA M564G mutant enzyme from yeast, pH 7.8, 30°C Rattus norvegicus
0.015
-
 butyryl-CoA M564G mutant enzyme from yeast, pH 7.8, 30°C Rattus norvegicus
0.022
-
 hexanoyl-CoA M564G mutant enzyme from yeast, pH 7.8, 30°C Rattus norvegicus
0.023
-
 acetyl-CoA recombinant enzyme from yeast, pH 7.8, 30°C Rattus norvegicus
0.025
-
 octanoyl-CoA recombinant enzyme from yeast, pH 7.8, 30°C Rattus norvegicus
0.03
-
 butyryl-CoA recombinant enzyme from yeast, pH 7.8, 30°C Rattus norvegicus
0.032
-
 acetyl-CoA M564G mutant enzyme from yeast, pH 7.8, 30°C Rattus norvegicus
0.033
-
 hexanoyl-CoA recombinant enzyme from yeast, pH 7.8, 30°C Rattus norvegicus
0.086
-
 carnitine M564G mutant enzyme from yeast with octanoyl-CoA as second substrate, pH 7.8, 30°C Rattus norvegicus
0.164
-
 carnitine M564G mutant enzyme from yeast with hexanoyl-CoA as second substrate, pH 7.8, 30°C Rattus norvegicus
0.17
-
 carnitine M564G mutant enzyme from yeast with butyryl-CoA as second substrate, pH 7.8, 30°C Rattus norvegicus
0.203
-
 carnitine recombinant enzyme from yeast with acetyl-CoA as second substrate, pH 7.8, 30°C Rattus norvegicus
0.22
-
 carnitine recombinant enzyme from yeast with butyryl-CoA as second substrate, pH 7.8, 30°C Rattus norvegicus
0.339
-
 carnitine M564G mutant enzyme from yeast with acetyl-CoA as second substrate, pH 7.8, 30°C Rattus norvegicus
0.567
-
 carnitine recombinant enzyme from yeast with hexanoyl-CoA as second substrate, pH 7.8, 30°C Rattus norvegicus
0.964
-
 carnitine recombinant enzyme from yeast with octanoyl-CoA as second substrate, pH 7.8, 30°C Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70800
-
 x * 70800, deduced from amino acid sequence Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + carnitine Rattus norvegicus modulates acetyl-CoA and CoA availability for a wide range of acyl-transfer reactions, involved in fatty acid metabolism CoA + O-acetylcarnitine
-
 r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q704S8
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of GST-fusion proteins from Escherichia coli Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + carnitine
-
 Rattus norvegicus CoA + O-acetylcarnitine
-
 r
acetyl-CoA + carnitine modulates acetyl-CoA and CoA availability for a wide range of acyl-transfer reactions, involved in fatty acid metabolism Rattus norvegicus CoA + O-acetylcarnitine
-
 r
butyryl-CoA + carnitine
-
 Rattus norvegicus CoA + O-butyrylcarnitine
-
 r
hexanoyl-CoA + carnitine very low activity Rattus norvegicus CoA + O-hexanoylcarnitine
-
 r
additional information wild type enzyme is almost inactive towards longer chain fatty acids, some mutations increases activity towards longer chain fatty acids Rattus norvegicus ?
-
 ?
octanoyl-CoA + carnitine very low activity Rattus norvegicus CoA + O-octanoylcarnitine
-
 r

Subunits

Subunits Comment Organism
? x * 70800, deduced from amino acid sequence Rattus norvegicus