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Literature summary for 2.3.1.7 extracted from

  • Miyazawa, S.; Ozasa, H.; Furuta, S.; Osumi, T.; Hashimoto, T.
    Purification and properties of carnitine acetyltransferase from rat liver (1983), J. Biochem., 93, 439-451.
    View publication on PubMed
Search for more literature for 2.3.1.7

General Stability

General Stability Organism
enzyme is stable throughout purification procedure Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxynonenal
-
 Rattus norvegicus
iodoacetic acid
-
 Rattus norvegicus
p-chloromercuriphenyl sulfonic acid
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 CoASH substrate hexanoylcarnitine, enzyme from liver homogenate Rattus norvegicus
0.024
-
 CoASH substrate decanoylcarnitine, enzyme from liver homogenate Rattus norvegicus
0.027
-
 CoASH substrate decanoylcarnitine, enzyme from mitochondria Rattus norvegicus
0.027
-
 CoASH substrate butyrylcarnitine and octanoylcarnitine, enzyme from liver homogenate Rattus norvegicus
0.028
-
 CoASH substrate acetylcarnitine, enzyme from liver homogenate Rattus norvegicus
0.032
-
 CoASH substrate acetylcarnitine, enzyme from mitochondria Rattus norvegicus
0.038
-
 CoASH substrate butyrylcarnitine, enzyme from mitochondria Rattus norvegicus
0.044
-
 CoASH substrate hexanoylcarnitine, enzyme from mitochondria Rattus norvegicus
0.054
-
 CoASH substrate octanoylcarnitine, enzyme from mitochondria Rattus norvegicus
0.39
-
 acetylcarnitine enzyme from liver homogenate Rattus norvegicus
0.48
-
 butyrylcarnitine enzyme from liver homogenate Rattus norvegicus
0.6
-
 acetylcarnitine enzyme from mitochondria Rattus norvegicus
0.64
-
 decanoylcarnitine enzyme from mitochondria Rattus norvegicus
0.66
-
 decanoylcarnitine enzyme from liver homogenate Rattus norvegicus
0.66
-
 butyrylcarnitine enzyme from mitochondria Rattus norvegicus
1.27
-
 octanoylcarnitine enzyme from mitochondria Rattus norvegicus
1.62
-
 octanoylcarnitine enzyme from liver homogenate Rattus norvegicus
2.28
-
 hexanoylcarnitine enzyme from liver homogenate Rattus norvegicus
2.51
-
 hexanoylcarnitine enzyme from mitochondria Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-
peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus
27000
-
 1 * 36500 + 1 * 27000, purified from mitochondrial fraction, monomeric form converted into dimeric by proteolytic modification after disruption of mitochondria, SDS-PAGE Rattus norvegicus
34000
-
 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus
36500
-
 1 * 36500 + 1 * 27000, purified from mitochondrial fraction, monomeric form converted into dimeric by proteolytic modification after disruption of mitochondria, SDS-PAGE Rattus norvegicus
56000
-
 purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate Rattus norvegicus
61900
-
 purified from mitochondrial fraction, gel filtration Rattus norvegicus
66100
-
 purified from liver homogenate, gel filtration Rattus norvegicus
67500
-
 purified from liver homogenate, SDS-PAGE Rattus norvegicus
69000
-
 translation product of the enzyme by the reticulocyte lysate protein-synthesizing system Rattus norvegicus
69000
-
 post-mitochondrial fraction, 2 polypeptides 67500 Da + 69000 Da cannot be separated from each other Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + carnitine Rattus norvegicus
-
 O-acetylcarnitine + CoASH
-
 r
acetyl-CoA + carnitine Columba sp.
-
 O-acetylcarnitine + CoASH
-
 r
acyl-CoA + carnitine Rattus norvegicus
-
 acylcarnitine + CoASH
-
 r
acyl-CoA + carnitine Columba sp.
-
 acylcarnitine + CoASH
-
 r
acylcarnitine + CoASH Rattus norvegicus
-
 acyl-CoA + carnitine
-
 r
acylcarnitine + CoASH Columba sp.
-
 acyl-CoA + carnitine
-
 r
O-acetylcarnitine + CoASH Rattus norvegicus
-
 acetyl-CoA + carnitine
-
 r
O-acetylcarnitine + CoASH Columba sp.
-
 acetyl-CoA + carnitine
-
 r

Organism

Organism UniProt Comment Textmining
Columba sp.
-
 pigeon
-
Rattus norvegicus
-
 rat
-

Purification (Commentary)

Purification (Comment) Organism
purified from mitochondrial fraction and from homogenate of frozen liver Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
liver
-
 Columba sp.
-
muscle
-
 Rattus norvegicus
-
muscle
-
 Columba sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
120
-
 purified from postmitochondrial fraction Rattus norvegicus
220
-
 purified from liver homogenate Rattus norvegicus
240
-
 purified from mitochondrial fraction Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 50 mM potassium phosphate buffer, pH 7.5, purified enzyme can be stored for several months without loss of activity Rattus norvegicus
-20°C, frozen mitochondrial fraction can be stored for several months without loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + carnitine
-
 Rattus norvegicus O-acetylcarnitine + CoASH
-
 r
acetyl-CoA + carnitine
-
 Columba sp. O-acetylcarnitine + CoASH
-
 r
acetyl-CoA + L-(-)carnitine
-
 Rattus norvegicus O-acetylcarnitine + CoASH
-
 r
acetyl-CoA + L-(-)carnitine
-
 Columba sp. O-acetylcarnitine + CoASH
-
 r
acyl-CoA + carnitine
-
 Rattus norvegicus acylcarnitine + CoASH
-
 r
acyl-CoA + carnitine
-
 Columba sp. acylcarnitine + CoASH
-
 r
acylcarnitine + CoASH
-
 Rattus norvegicus acyl-CoA + carnitine
-
 r
acylcarnitine + CoASH
-
 Columba sp. acyl-CoA + carnitine
-
 r
decanoyl-CoA + carnitine
-
 Rattus norvegicus decanoylcarnitine + CoASH
-
 r
decanoyl-CoA + carnitine
-
 Columba sp. decanoylcarnitine + CoASH
-
 r
decanoylcarnitine + CoASH
-
 Rattus norvegicus decanoyl-CoA + carnitine
-
 r
decanoylcarnitine + CoASH
-
 Columba sp. decanoyl-CoA + carnitine
-
 r
hexanoyl-CoA + carnitine
-
 Rattus norvegicus hexanoylcarnitine + CoASH
-
 r
hexanoyl-CoA + carnitine
-
 Columba sp. hexanoylcarnitine + CoASH
-
 r
hexanoylcarnitine + CoASH
-
 Rattus norvegicus hexanoyl-CoA + carnitine
-
 r
hexanoylcarnitine + CoASH
-
 Columba sp. hexanoyl-CoA + carnitine
-
 r
n-butyryl-CoA + carnitine
-
 Rattus norvegicus n-butyrylcarnitine + CoASH
-
 r
n-butyrylcarnitine + CoASH
-
 Rattus norvegicus n-butyryl-CoA + carnitine
-
 r
O-acetylcarnitine + CoASH
-
 Rattus norvegicus acetyl-CoA + L-(-)carnitine
-
 r
O-acetylcarnitine + CoASH
-
 Columba sp. acetyl-CoA + L-(-)carnitine
-
 r
O-acetylcarnitine + CoASH
-
 Rattus norvegicus acetyl-CoA + carnitine
-
 r
O-acetylcarnitine + CoASH
-
 Columba sp. acetyl-CoA + carnitine
-
 r
octanoyl-CoA + carnitine
-
 Rattus norvegicus octanoylcarnitine + CoASH
-
 r
octanoylcarnitine + CoASH
-
 Rattus norvegicus octanoyl-CoA + carnitine
-
 r

Subunits

Subunits Comment Organism
dimer 1 * 36500 + 1 * 27000, purified from mitochondrial fraction, monomeric form converted into dimeric by proteolytic modification after disruption of mitochondria, SDS-PAGE Rattus norvegicus
dimer 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 25 stored in a refrigerator for 2 days or incubated at 25°C for 5 h, monomeric form remains unchanged Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Rattus norvegicus