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Literature summary for 2.3.1.7 extracted from
- Peroxisomal and microsomal carnitine acetyltransferases (1981), Methods Enzymol., 71, 351-358.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Clofibrate | mitochondrial enzyme increases in specific activity | Rattus norvegicus |  |
| streptozotocin | increases enzyme in liver | Rattus norvegicus | |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Columba sp. |
General Stability
| General Stability | Organism |
|---|---|
| enzyme is abolished by detergents, hypotonic media or freeze-thawing | Columba sp. |
| very unstable | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
CoASH | - |
Columba sp. | |
| 0.069 | - |
CoASH | - |
Rattus norvegicus | |
| 0.12 | - |
L-(-)-carnitine | - |
Columba sp. | |
| 0.15 | - |
L-(-)-carnitine | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | smooth and rough | Columba sp. | 5783 | - |
| microsome | - |
Columba sp. | - |
- |
| microsome | membrane bound | Rattus norvegicus | - |
- |
| mitochondrion | - |
Cavia porcellus | 5739 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
| peroxisome | - |
Rattus norvegicus | 5777 | - |
| soluble | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE | Rattus norvegicus |
| 34000 | - |
1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE | Rattus norvegicus |
| 51000 | - |
- |
Columba sp. |
| 59000 | - |
microsomal and peroxisomal enzyme | Rattus norvegicus |
| 62000 | - |
- |
Rattus norvegicus |
| 62000 | - |
- |
Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + carnitine | Cavia porcellus | - |
O-acetylcarnitine + CoASH | - |
r | |
| acetyl-CoA + carnitine | Rattus norvegicus | - |
O-acetylcarnitine + CoASH | - |
r | |
| acetyl-CoA + carnitine | Sus scrofa | - |
O-acetylcarnitine + CoASH | - |
r | |
| acetyl-CoA + carnitine | Bos taurus | - |
O-acetylcarnitine + CoASH | - |
r | |
| acetyl-CoA + carnitine | Columba sp. | - |
O-acetylcarnitine + CoASH | - |
r | |
| acyl-CoA + carnitine | Cavia porcellus | - |
acylcarnitine + CoASH | - |
r | |
| acyl-CoA + carnitine | Rattus norvegicus | - |
acylcarnitine + CoASH | - |
r | |
| acyl-CoA + carnitine | Sus scrofa | - |
acylcarnitine + CoASH | - |
r | |
| acyl-CoA + carnitine | Bos taurus | - |
acylcarnitine + CoASH | - |
r | |
| acyl-CoA + carnitine | Columba sp. | - |
acylcarnitine + CoASH | - |
r | |
| acylcarnitine + CoASH | Cavia porcellus | - |
acyl-CoA + carnitine | - |
r | |
| acylcarnitine + CoASH | Rattus norvegicus | - |
acyl-CoA + carnitine | - |
r | |
| acylcarnitine + CoASH | Sus scrofa | - |
acyl-CoA + carnitine | - |
r | |
| acylcarnitine + CoASH | Bos taurus | - |
acyl-CoA + carnitine | - |
r | |
| acylcarnitine + CoASH | Columba sp. | - |
acyl-CoA + carnitine | - |
r | |
| O-acetylcarnitine + CoASH | Cavia porcellus | - |
acetyl-CoA + carnitine | - |
r | |
| O-acetylcarnitine + CoASH | Rattus norvegicus | - |
acetyl-CoA + carnitine | - |
r | |
| O-acetylcarnitine + CoASH | Sus scrofa | - |
acetyl-CoA + carnitine | - |
r | |
| O-acetylcarnitine + CoASH | Bos taurus | - |
acetyl-CoA + carnitine | - |
r | |
| O-acetylcarnitine + CoASH | Columba sp. | - |
acetyl-CoA + carnitine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
beef | - |
| Cavia porcellus | - |
guinea pig | - |
| Columba sp. | - |
pigeon | - |
| Rattus norvegicus | - |
rat | - |
| Sus scrofa | - |
pig | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
- |
Columba sp. |
| partial | Rattus norvegicus |
| partially | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| breast muscle | - |
Columba sp. | - |
| heart | - |
Rattus norvegicus | - |
| heart | - |
Sus scrofa | - |
| heart | - |
Bos taurus | - |
| heart | - |
Columba sp. | - |
| kidney | - |
Rattus norvegicus | - |
| kidney | - |
Sus scrofa | - |
| liver | - |
Cavia porcellus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Columba sp. | - |
| muscle | - |
Rattus norvegicus | - |
| muscle | - |
Columba sp. | - |
| skeletal muscle | - |
Rattus norvegicus | - |
| small intestine | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.005 | - |
liver | Rattus norvegicus |
| 0.25 | - |
skeletal muscle | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, peroxisomal and microsomal fractions from isopycnic sucrose density gradients, activity is stable during storage for at least 3 months | Rattus norvegicus |
| -20°C, peroxisomes and microsomes stored in 0.4 M KCl, 0.02% sodium azide, 150 mM Tris-HCl, pH, retains at least 95% of their activity when stored for 3 months | Rattus norvegicus |
| 4°C, enzyme is abolished by prolonged storage | Columba sp. |
| 4°C, peroxisomal and microsomal fractions from isopycnic sucrose density gradients, activity is stable during storage for 10 days | Rattus norvegicus |
| 4°C, peroxisomes and microsomes stored in 0.4 M KCl, 0.02% sodium azide, 150 mM Tris-HCl, pH, retains at least 95% of their activity when stored for 18 days | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetoacetyl-CoA + L-(-)carnitine | - |
Rattus norvegicus | O-acetoacetyl(-)carnitine + CoASH | - |
r | |
| acetyl-CoA + carnitine | - |
Cavia porcellus | O-acetylcarnitine + CoASH | - |
r | |
| acetyl-CoA + carnitine | - |
Rattus norvegicus | O-acetylcarnitine + CoASH | - |
r | |
| acetyl-CoA + carnitine | - |
Sus scrofa | O-acetylcarnitine + CoASH | - |
r | |
| acetyl-CoA + carnitine | - |
Bos taurus | O-acetylcarnitine + CoASH | - |
r | |
| acetyl-CoA + carnitine | - |
Columba sp. | O-acetylcarnitine + CoASH | - |
r | |
| acetyl-CoA + L-(-)carnitine | - |
Cavia porcellus | O-acetylcarnitine + CoASH | - |
r | |
| acetyl-CoA + L-(-)carnitine | - |
Rattus norvegicus | O-acetylcarnitine + CoASH | - |
r | |
| acetyl-CoA + L-(-)carnitine | - |
Sus scrofa | O-acetylcarnitine + CoASH | - |
r | |
| acetyl-CoA + L-(-)carnitine | - |
Bos taurus | O-acetylcarnitine + CoASH | - |
r | |
| acetyl-CoA + L-(-)carnitine | - |
Columba sp. | O-acetylcarnitine + CoASH | - |
r | |
| acyl-CoA + carnitine | - |
Cavia porcellus | acylcarnitine + CoASH | - |
r | |
| acyl-CoA + carnitine | - |
Rattus norvegicus | acylcarnitine + CoASH | - |
r | |
| acyl-CoA + carnitine | - |
Sus scrofa | acylcarnitine + CoASH | - |
r | |
| acyl-CoA + carnitine | - |
Bos taurus | acylcarnitine + CoASH | - |
r | |
| acyl-CoA + carnitine | - |
Columba sp. | acylcarnitine + CoASH | - |
r | |
| acylcarnitine + CoASH | - |
Cavia porcellus | acyl-CoA + carnitine | - |
r | |
| acylcarnitine + CoASH | - |
Rattus norvegicus | acyl-CoA + carnitine | - |
r | |
| acylcarnitine + CoASH | - |
Sus scrofa | acyl-CoA + carnitine | - |
r | |
| acylcarnitine + CoASH | - |
Bos taurus | acyl-CoA + carnitine | - |
r | |
| acylcarnitine + CoASH | - |
Columba sp. | acyl-CoA + carnitine | - |
r | |
| additional information | - |
Sus scrofa | ? | - |
? | |
| additional information | - |
Columba sp. | ? | - |
? | |
| additional information | O-acetyl-(+)-carnitine and reduced glutathione will not serve as substrates, succinyl-CoA or beta-hydroxy-betamethylglutaryl-CoA are no substrates | Rattus norvegicus | ? | - |
? | |
| O-acetoacetyl(-)carnitine + CoASH | - |
Rattus norvegicus | acetoacetyl-CoA + L-(-)carnitine | - |
r | |
| O-acetylcarnitine + CoASH | - |
Cavia porcellus | acetyl-CoA + L-(-)carnitine | - |
r | |
| O-acetylcarnitine + CoASH | - |
Rattus norvegicus | acetyl-CoA + L-(-)carnitine | - |
r | |
| O-acetylcarnitine + CoASH | - |
Sus scrofa | acetyl-CoA + L-(-)carnitine | - |
r | |
| O-acetylcarnitine + CoASH | - |
Bos taurus | acetyl-CoA + L-(-)carnitine | - |
r | |
| O-acetylcarnitine + CoASH | - |
Columba sp. | acetyl-CoA + L-(-)carnitine | - |
r | |
| O-acetylcarnitine + CoASH | - |
Cavia porcellus | acetyl-CoA + carnitine | - |
r | |
| O-acetylcarnitine + CoASH | - |
Rattus norvegicus | acetyl-CoA + carnitine | - |
r | |
| O-acetylcarnitine + CoASH | - |
Sus scrofa | acetyl-CoA + carnitine | - |
r | |
| O-acetylcarnitine + CoASH | - |
Bos taurus | acetyl-CoA + carnitine | - |
r | |
| O-acetylcarnitine + CoASH | - |
Columba sp. | acetyl-CoA + carnitine | - |
r | |
| propionyl-CoA + L-(-)carnitine | - |
Rattus norvegicus | propionylcarnitine + CoASH | - |
r | |
| propionyl-CoA + L-(-)carnitine | - |
Bos taurus | propionylcarnitine + CoASH | - |
r | |
| propionylcarnitine + CoASH | - |
Rattus norvegicus | propionyl-CoA + L-(-)carnitine | - |
r | |
| propionylcarnitine + CoASH | - |
Bos taurus | propionyl-CoA + L-(-)carnitine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | microsomal transferase is more stable at pH 7.5 than at pH 6.0 | Rattus norvegicus |
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